W. James Gooderham

TL;DR: The identification and characterization of a novel P. aeruginosa two-component regulator affecting polymyxin-adaptive resistance, ParR-ParS (PA1799-PA1798), which was required for activation of the arnBCADTEF LPS modification operon in the presence of subinhibitory concentrations of polymyXin, colistin, or the bovine peptide indolicidin, leading to increased resistance to various polycationic

TL;DR: In this paper, the authors summarize the current body of knowledge of these and other two-component systems that provides insight into the complex regulation of virulence and resistance in Pseudomonas aeruginosa.

TL;DR: This report describes the construction of a mini-Tn5-luxCDABE mutant library and a high-throughput inverse PCR method to amplify DNA flanking the site of insertion for sequencing and insertion site mapping and demonstrates the utility of chromosomal lux fusions.

TL;DR: It was demonstrated that, following exposure to inducing antimicrobial peptides, cprRS mutants did not become adaptively resistant to polymyxins as was observed for wild-type cells, and these findings provide greater insight into the complex regulation of LPS modification in Pseudomonas aeruginosa.

TL;DR: This study demonstrated that mutation of phoQ caused reduced twitching motility, biofilm formation and rapid attachment to surfaces, 2.2-fold reduced cytotoxicity to human lung epithelial cells, substantially reduced lettuce leaf virulence, and a major, 10 000-fold reduction in competitiveness in chronic rat lung infections.