Showing papers by "Warren L. Butler published in 1985"
TL;DR: In this article, the authors measured the fluorescence lifetimes of spinach chloroplasts with a modelocked dye laser and time-correlated single photon counting, revealing information about energy transport and functional organization of the chlorplasts.
Abstract: — Fluorescence lifetimes of spinach chloroplasts were measured with a modelocked dye laser and time-correlated single photon counting. Information about energy transport and functional organization of the chloroplasts is revealed by such time-resolved fluorescence studies. Quenching experiments using treatment with UV light or the chemical agent dibromothymoquinone are consistent with the notion that there is heterogeneity associated with PS II units and that such heterogeneity is reflected over the entire time range of fluorescence decay, not just in a single component. Phosphorylation experiments were also carried out which permit us to relate these kinetic studies to previous steady state observations.
38 citations
TL;DR: In this paper, three models are defined explicitly, solved rigorously, and compared in detail with experimental data, and only the heterogeneous bipartite model has solutions which agree with commonly agreed upon features of the data.
Abstract: — Time-resolved fluorescence decay profiles following picosecond excitation of plant chloro-plasts or algae are now being measured with high precision and reproducibility. A number of suggestions have been put forward to explain the complex decay kinetics which are observed. In this paper three models are defined explicitly, solved rigorously, and compared in detail with experimental data. The complete description of energy transport in chloroplasts is no doubt very complicated, and only such quantitative comparisons will make it possible to decide which models are consistent with experiments. Of the three models examined, only the heterogeneous bipartite model has solutions which agree with commonly agreed upon features of the data. It offers, therefore, one starting point for more elaborate models and a guide to the design of further experiments.
23 citations