W
Wenbiao Chen
Researcher at Vanderbilt University
Publications - 71
Citations - 7920
Wenbiao Chen is an academic researcher from Vanderbilt University. The author has contributed to research in topics: Zebrafish & Gene. The author has an hindex of 32, co-authored 57 publications receiving 7026 citations. Previous affiliations of Wenbiao Chen include Oregon Health & Science University & Vanderbilt University Medical Center.
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Journal ArticleDOI
Efficient multiplex biallelic zebrafish genome editing using a CRISPR nuclease system
TL;DR: An improved CRISPR/Cas system in zebra fish with custom guide RNAs and a zebrafish codon-optimized Cas9 protein that efficiently targeted a reporter transgene Tg(-5.1mnx1:egfp) and four endogenous loci and five genomic loci, resulting in multiple loss-of-function phenotypes in the same injected fish.
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Agouti protein is an antagonist of the melanocyte-stimulating-hormone receptor.
Dongsi Lu,Derril H. Willard,I R Patel,Sue H. Kadwell,Laurie K. Overton,T. A. Kost,Michael A. Luther,Wenbiao Chen,Richard P. Woychik,William O. Wilkison,Roger D. Cone +10 more
TL;DR: Agouti protein is used to demonstrate that agouti is a high-affinity antagonist of the MSH receptor and blocks α-MSH stimulation of adenylyl cyclase, the effector through which α- MSH induces eumelanin synthesis.
Journal ArticleDOI
Insertional mutagenesis in zebrafish rapidly identifies genes essential for early vertebrate development.
Gregory Golling,Adam Amsterdam,Zhaoxia Sun,Marcelo Antonelli,Ernesto Maldonado,Wenbiao Chen,Shawn M. Burgess,Maryann Haldi,Karen Artzt,Sarah Farrington,Shuh-Yow Lin,Robert M. Nissen,Nancy Hopkins +12 more
TL;DR: A large-scale, insertional mutagenesis screen in zebrafish is carried out, using mouse retroviral vectors as the mutagen, providing an unbiased view of the genetic construction kit for a vertebrate embryo and revealing the diversity of genes required for vertebrate development.
Journal Article
The melanocortin receptors: agonists, antagonists, and the hormonal control of pigmentation
Roger D. Cone,Dongsi Lu,Sandhya V. Koppula,Dag Inge Våge,Helge Klungland,Bruce A. Boston,Wenbiao Chen,David N. Orth,Colin W. Pouton,Robert A. Kesterson +9 more
TL;DR: The first two melanocortin receptors were identified in the mouse and the first two were the melanocyte-stimulating hormone receptor (MSH-R) and adrenocorticotropin receptor (ACTH-R), respectively.
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A large-scale insertional mutagenesis screen in zebrafish.
Adam Amsterdam,Shawn M. Burgess,Gregory Golling,Wenbiao Chen,Zhaoxia Sun,Karen Townsend,Sarah Farrington,Maryann Haldi,Nancy Hopkins +8 more
TL;DR: A large-scale insertional mutagenesis screen in the zebrafish with the goal of isolating approximately 1000 embryonic mutations and cloning a significant fraction of the mutated genes, as these are the genes important for normal embryogenesis of a vertebrate.