Wenjuan Zha

TL;DR: The use of various metabolic engineering strategies to redirect the carbon flux inside E. coli to pathways responsible for the generation of malonyl-CoA led to the creation of an E. Escherichia coli strain with 15-fold elevated cellular malony lCoA level, which should be highly useful for improved production of important natural products where the cellular maloneyl- CoA level is rate-limiting.

TL;DR: Comparison of different promoters for the expression of g2ps1 in S. cerevisiae indicates that the alcohol dehydrogenase II promoter (PADH2) affords the highest expression levels of 2‐PS, and the genes encoding the various TAL‐synthesizing enzyme activities are expressed in S.

TL;DR: The recombination efficiency of the StEP method is comparable to that of the most widely used in vitro DNA recombination method, DNA shuffling, but does not require DNA fragmentation and can be carried out in a single tube.

TL;DR: In this paper, a homology structural model of PhlD showed the presence of a buried tunnel extending out from the active site to assist the binding of long chain acyl-CoAs, which suggested novel strategies for combinatorial biosynthesis of unnatural pharmaceutically important polyketides.

TL;DR: The rational design of a fatty acid biosynthetic pathway, Brevibacterium ammoniagenes fatty acid synthase B (FAS-B), that allows the microbial synthesis of triacetic acid lactone (TAL) from an inexpensive feedstock, d-glucose, represents a novel strategy for the benzene-free synthesis of aromatic chemicals.