Werner Ens

TL;DR: A "MS BLAST" homology searching protocol was developed to overcome specific limitations imposed by mass spectrometric data, such as the limited accuracy of de novo sequence predictions.

TL;DR: A combination of the nanoelectrospray ion source, isotopic end labeling of peptides and a quadrupole/ time-of-flight instrument allows facile read-out of the sequences of tryptic peptides.

TL;DR: A matrix-assisted laser desorption/ionization source has been coupled to a tandem quadrupole/time-of-flight (QqTOF) mass spectrometer by means of a collisional damping interface and Ion production at pressures near 1 Torr is found to give reduced metastable fragmentation, particularly for higher mass molecular ions.

TL;DR: The proposed model is based on the measurement of the retention times of 346 tryptic peptides in the 560- to 4,000-Da mass range, derived from a mixture of 17 protein digests, and can be used for accurate prediction of retention times fortryptic peptide on reversed-phase columns of different sizes with a linear water-ACN gradient and with TFA as the ion-pairing modifier.

TL;DR: The high mass resolution and the high mass accuracy of this instrument in both MS and MS/MS modes allow identification of a protein either by peptide mass fingerprinting of the protein digest or from tandem mass spectra acquired by collision-induced dissociation of individual peptide precursors.