Showing papers by "William B. Miller published in 1994"
TL;DR: In this article, the specific sugars commonly found in cotton fiber are determined to be caused by honeydew deposits from phloem-feeding insects, including whiteflies and aphids.
Abstract: Stickiness of cotton fiber is usually caused by honeydew deposits from phloem- feeding insects, including whiteflies and aphids. This work was conducted to determine if the specific sugars commonly...
26 citations
TL;DR: Three invertase forms (EC 3.2.1.26), identified in soluble extracts from developing flower buds of Lilium longiflorum Thunb, showed no sucrose:sucrose fructosyltransferase activity and Cu2-showed differential partial inhibition.
Abstract: Three invertase forms (EC 3.2.1.26) were identified in soluble extracts from developing flower buds of Lilium longiflorum Thunb. cv. Nellie White. The enzymes were separable on a diethylaminoethyl (DEAE)-Sephacel column and designated invertase I. II or III according to the order of elution from Sephacel. To determine tissue specificity of these floral invertases, anthers were separated from tepal. pistil and filament tissue, and analyzed for invertase activity. Invertase I was localized primarily in anthers, with invertases II and III being present in much smaller amounts (less than 5% of the invertase I activity). Much higher levels of invertases II and III were found in the nonanther organs of the flower, where essentially no invertase 1 was detectable. Further purification of each form (using gel filtration. Con-A-Sepharose affinity chromatog-raphy and hydrophobic interaction chromatography on phenyl-agarose) resulted in 135- 189- and 202-fold purification of pooled fractions from DEAE-Sephacel. respectively, and established that each invertase form is a glycoprotein. Each was an acid invertase. with pH optima between 4.0 and 5.0 and an apparent molecular mass of 77 500 Da (as determined by Sephadex gel filtration). The invertases had sucrose Km values of 1.0. 6.4 and 6.6 mM. and temperature optima of 40. 50 and 45°C. respectively. A temperature stability study revealed that invertase III was the most thermostable, followed by II and I. Invertases II and III had lower affinity to raffinose and stachyose than invertase I. All three enzymes were completely inhibited by Hg2+ or Ag+ ions at 1.7 mM. At this concentration. Cu2-showed differential partial inhibition. Although fructan was shown to be present in both anther and nonanther tissues of Lilium flower buds, these invertases showed no sucrose:sucrose fructosyltransferase (EC 2.4.1.99) activity.
25 citations
TL;DR: Although CO2 enrichment increased the carbohydrate pools of the plants, the results do not support the hypothesis that increased carbohydrate supply plays a role in reduction of postharvest leaf chlorosis of potted miniature roses.
3 citations