Showing papers by "William B. Miller published in 1998"
TL;DR: Although all three enzymes capable of catalyzing sucrose cleavage were present in every flower organ of L. longiflorum, soluble invertsase was the predominant enzyme in all flower organs except stigma where cell wall invertase dominated.
Abstract: The activities of soluble invertase (EC 3.2.1.26), cell wall invertase (EC 3.2.1.26) and sucrose synthase (EC 2.4.1.13) were determined in Easter lily (Lilium longiflorum Thunb. cv. Nellie White) floral organs during flower development. These enzyme activities were correlated with dry weight gains and carbohydrate pools to investigate the importance of their expression in maintaining sink strength of floral organs. In the early stages of flower bud development, anthers exhibited the highest rates of dry weight gain and activity of sucrolytic enzymes. Once anther growth was completed, the dry weight gain of tepal, filament, stigma and style increased with a concomitant increase in hexose concentrations and invertase activity. Although all three enzymes capable of catalyzing sucrose cleavage were present in every flower organ of L. longiflorum, soluble invertase was the predominant enzyme in all flower organs except stigma where cell wall invertase dominated. Soluble invertase activity was highly correlated with dry weight gain in most of the flower organs.
90 citations
56 citations
TL;DR: Symptoms of Fe toxicity in marigold developed only in the high-Fe treatment and relative cultivar susceptibility to Fe toxicity was Orange Jubilee > First Lady > Orange Lady > Yellow Galore > Gold Lady > Marvel Gold > Discovery Orange.
Abstract: The susceptibility of seven African marigold (Tagetes erecta L.) cultivars to iron toxicity was assessed. Plants were grown in a greenhouse in a soilless medium and Fe-DTPA was incorporated into the nutrient solution at either 0.018 mmol.L -1 (low) or 0.36 mmol.L -1 (high). Symptoms of Fe toxicity (bronze speckle disorder in marigold characterized by chlorotic and necrotic speckling and downward leaf cupping and curling) developed only in the high-Fe treatment. The concentration of Fe in leaves in the high-Fe treatment was 5.6 and 1.7 times as great as in the low-Fe treatment for 'Orange Jubilee' and 'Discovery Orange', respectively. Based upon the percentage of plants affected and leaf symptom severity, relative cultivar susceptibility to Fe toxicity was Orange Jubilee > First Lady > Orange Lady > Yellow Galore > Gold Lady > Marvel Gold > Discovery Orange. Chemical names used: ferric diethylenetriaminepentaacetic acid, disodium salt dihydrate (Fe-DTPA).
16 citations
TL;DR: Three soluble invertase (EC 3.2.1.26) isoforms from Easter lily flower buds were purified to apparent homogeneity and antibodies against wheat coleoptile acid invertedase recognized only 56- and 54-kDa bands of invertases II and III.
Abstract: Three soluble invertase (EC 3.2.1.26) isoforms from Easter lily (Lilium longiflorum Thunb. cv. Nellie White) flower buds were purified to apparent homogeneity. Non-denaturing PAGE showed one band for all three invertases that corresponded to the invertase activity. SDS-PAGE of purified invertase I gave a single band at 78 kDa, whereas invertases II and III gave three bands at 54, 52 and 24 kDa. Antibodies against tomato fruit acid invertase and Urtica dioica leaf acid invertase recognized all three invertase isoforms, whereas antibodies against wheat coleoptile acid invertase recognized only 56- and 54-kDa bands of invertases II and III. Antibodies against wheat coleoptile invertase recognized the 54- and 52-kDa proteins from crude extracts of all flower organs, and a 72-kDa protein in both leaf and bulb scale extracts. All three invertases bound to Con-A peroxidase. Deglycosylation of invertase I with glycopeptidase F was complete and resulted in a peptide of 75 kDa. Invertases II and III were deglycosylated partially by glycopeptidase F and resulted in proteins of 53, 51, 50 and 22 kDa. Invertase I was localized only in anther and filament, whereas the other two isoforms were present in all flower organs.
6 citations
2 citations