W
William J. Dower
Researcher at Bio-Rad Laboratories
Publications - 5
Citations - 3215
William J. Dower is an academic researcher from Bio-Rad Laboratories. The author has contributed to research in topics: Electroporation & Receptor. The author has an hindex of 4, co-authored 5 publications receiving 3142 citations.
Papers
More filters
Journal ArticleDOI
High efficiency transformation of E.coli by high voltage electroporation
TL;DR: E. coli can be transformed to extremely high efficiencies by subjecting a mixture of cells and DNA to brief but intense electrical fields of exponential decay waveform (electroporation), and most of the surviving cells are competent with up to 80% transformed at high DNA concentration.
Journal ArticleDOI
High-voltage electroporation of bacteria: genetic transformation of Campylobacter jejuni with plasmid DNA
TL;DR: It is demonstrated that the application of high-voltage discharges to bacterial cells permits genetic transformation, and evidence is presented that indicates that C. jejuni possesses DNA restriction and modification systems.
Patent
Electroporation of prokaryotic cells
TL;DR: In this article, the authors applied high-intensity electric fields of short duration to reversibly permeabilize the cell walls and achieved very high transformation efficiencies in the range from 10 9 to 10 10 cells/μg and transformation frequencies approaching 80% and above.
Journal ArticleDOI
Synthetic peptide ligands of the antigen binding receptor induce programmed cell death in a human B-cell lymphoma
TL;DR: The ligands, when conjugated to form dimers or tetramers, induced cell death by apoptosis in vitro with an IC50 between 40 and 200 nM and was associated with specific stimulation of intracellular protein tyrosine phosphorylation.