Xihua Wu

TL;DR: The citric acid yield and the conversion rate of glucose of chsC-3 were both improved by 3.6%, when compared with that of the original strain in the stirred tank bioreactor, and the high-yield strain chsD-3 exhibited excellentcitric acid production potential in the scale-up process.

TL;DR: When PLA 1 was bio-imprinted with 5% soybean lecithin at pH 5.0, its phospholipase (PLA) activity in leCithin–hexane solution increased by 30.9-fold and its substrate specificity was substantially enhanced, and a further increase in PLA activity of the bio-IMprinted PLA 1 (bi-PLA 1 ) was obtained.

TL;DR: In this article, the authors reported an increase in PLA activity in lecithin-hexane solution by 30.9-fold and its substrate specificity was substantially enhanced when 105mg diatomite/mg bi-PLA1 was incorporated as an immobilization carrier.

TL;DR: Prenylated quinones, especially menaquinones, have significant physiological activities, but are arduous to synthesize efficiently, due to the relaxed aromatic substrate specificity and prenylation regiospecificity at the ortho- site of the phenolic hydroxyl group, the aromatic preNyltransferase NovQ from Streptomyces may be useful in menaquinone synthesis from menadione.

TL;DR: In this paper, a method for extracting a microbial wet cell intracellular vitamin K2 crude product, which comprises the following steps: (1) concentrating a fermentation liquid through a membrane, and centrifugating to obtain wet cells; (2) adding a dehydrating organic solvent into the wet cells, stirring uniformly, carrying out pretreatment, centrifugation, and absorbing the supernate to obtain a primary extract; (3) adding an organic extractant into the dry cells subjected to primary extraction, standing, centrifuge, and absorption to obtain the secondary extract;