Open AccessJournal Article
Enhancing the performance of a phospholipase A1 for oil degumming by bio-imprinting and immobilization B Enzymatic
Li Zhemin,Hui Liu,Zhao Genhai,Peng Wang,Wang Li,Hefang Wu,Fang Xue,Sun Xiaowen,Xihua Wu,Zheng Zhiming +9 more
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TLDR
In this article, the authors reported an increase in PLA activity in lecithin-hexane solution by 30.9-fold and its substrate specificity was substantially enhanced when 105mg diatomite/mg bi-PLA1 was incorporated as an immobilization carrier.About:
This article is published in Journal of Molecular Catalysis.The article was published on 2016-01-01 and is currently open access. It has received 13 citations till now. The article focuses on the topics: Soybean oil.read more
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Enzyme-Coated Micro-Crystals: An Almost Forgotten but Very Simple and Elegant Immobilization Strategy
Rodolpho R. C. Monteiro,José C. S. dos Santos,Andrés R. Alcántara,Roberto Fernandez-Lafuente +3 more
TL;DR: This review tried to outline the possibilities of this useful immobilization strategy of enzymes using protein coated micro-crystals, which is underutilized even when the publications using it systematically presented a better performance of them in organic solvents than that of many other immobilized biocatalysts.
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Latest advances in degumming feedstock oils for large-scale biodiesel production
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Ultrasound assisted enzymatic degumming of crude soybean oil.
Nishant S. More,Parag R. Gogate +1 more
TL;DR: The approach of UAED was established to show much higher efficacy for soybean oil processing as compared to only ultrasound or only enzymatic treatment, and suitable reduction in acid value and oxidative stability is effectively obtained.
Journal ArticleDOI
Preparation and Characterization of Cellulose Triacetate as Support for Lecitase Ultra Immobilization
Francielle Batista da Silva,Wilson Galvão de Morais Júnior,Cleuzilene Vieira da Silva,Andressa Tironi Vieira,Antonio Carlos Ferreira Batista,Anizio M. Faria,Rosana Maria Nascimento de Assunção +6 more
TL;DR: The results showed that CTA-LU is a promising system for enzymatic reactions, being superior of the free enzyme and increase of the catalytic activity in the whole range of pH values.
Journal ArticleDOI
Lipase Immobilization through the Combination of Bioimprinting and Cross-Linked Protein-Coated Microcrystal Technology for Biodiesel Production
TL;DR: Results indicated that IM-CLPCMC exhibited improved thermal stability and long-term stability in organic solvents.
References
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Industrial enzyme applications.
TL;DR: The development of enzymes with improved properties for established technical applications and in the production of new enzymes tailor-made for entirely new areas of application where enzymes have not previously been used are studied.
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Why are enzymes less active in organic solvents than in water
TL;DR: In order to exploit fully the biotechnological opportunities afforded by nonaqueous enzymology, the issue of often drastically diminished enzymatic activity in organic solvents compared with that in water must be addressed and resolved as mentioned in this paper.
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Inhibitor-induced enzyme activation in organic solvents.
TL;DR: The enzymatic activity of the protease subtilisin in anhydrous organic solvents can be dramatically increased by pretreating the enzyme before it is placed in the nonaqueous medium.
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Enzymatic oil‐degumming by a novel microbial phospholipase
TL;DR: In laboratory tests the new phospholipase Lecitase® Novo has proven to be superior to porcine pancreatic LecItase® 10L and other phosphol Pipase A1 with respect to oil-degumming performance, and it has proved to be suited for degumming of different oil qualities ranging from water- Degummed to crude oil.
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Kinetics of reactions catalyzed by enzymes in solutions of surfactants
TL;DR: Differences and similarities between processes taking place in aqueous and organic solvents are highlighted, and the main models currently employed to interpret the results are briefly discussed.