Y
Yang Gyun Kim
Researcher at Johns Hopkins University
Publications - 8
Citations - 2471
Yang Gyun Kim is an academic researcher from Johns Hopkins University. The author has contributed to research in topics: Medicine & Restriction enzyme. The author has an hindex of 4, co-authored 4 publications receiving 2212 citations.
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Hybrid restriction enzymes: zinc finger fusions to Fok I cleavage domain
TL;DR: The deliberate creation of novel site-specific endonucleases by linking two different zinc finger proteins to the cleavage domain of Fok I endonuclease and the zinc finger motifs opens the way to generate many new enzymes with tailor-made sequence specificities desirable for various applications.
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Chimeric restriction endonuclease.
TL;DR: The construction of a chimeric restriction endonuclease is reported by linking the Drosophila Ultrabithorax homeodomain to the cleavage domain (FN) of Fok I restriction end onuclease, which shows the same DNA sequence-binding preference as that of Ubx.
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Site-specific cleavage of dna-rna hybrids by zinc finger/foki cleavage domain fusions
TL;DR: Zinc-finger proteins of the Cys2His2 type bind DNA-RNA hybrids with affinities comparable to those for DNA duplexes and exhibit site-specific cleavage of the DNA strand within DNA- RNA hybrids albeit at a lower efficiency.
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Overproduction, purification and characterization of M·HinfI methyltransferase and its deletion mutant
TL;DR: DNA methylation experiments with purified enzymes indicate that while wt enzyme methylates both forms of DNA substrates, the mutant enzyme appears to preferentially methylate ss DNA substrate.
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Korean Society of Nephrology 2022 Recommendations on controversial issues in diagnosis and management of hyponatremia
Yeonhee Lee,Kyung Don Yoo,Seon Ha Baek,Yang Gyun Kim,Hyojin Kim,Jiwon Ryu,Jin Hyuk Paek,Sang Heon Suh,Se Won Oh,Jeong-Yeol Lee,Jong Hyun Jhee,Jin-Soon Suh,Eun Mi Yang,Young Ho Park,Yae Lim Kim,Mi-Rai Choi,Kook Hwan Oh,Sejoong Kim +17 more
TL;DR: The committee issued conditional recommendations in favor of rapid intermittent bolus administration of hypertonic saline in severe hyponatremia, the use of vasopressin receptor antagonists in heart failure with hypervolemic hyponatsia, and syndrome of inappropriate antidiuresis.