Cinnamaldehyde and its derivatives, a novel class of antifungal agents.

A review of cinnamaldehyde and its derivatives as antibacterial agents.

Certain types of nanoparticles, especially zinc oxide nanoparticles (ZnONPs), are widely reported to be capable of the inhibition of harmful bacteria, yeasts, and filamentous fungi. The unique physicochemical and biological properties of ZnONPs also make them attractive to the food industry for use as a promising antifungal agent. This Review thoroughly introduces the preparation methods and antifungal properties of ZnONPs and analyzes their possible antifungal mechanisms. The applicability of ZnONPs in food packaging and nutritional supplements and as an antimicrobial additive is also documented. Moreover, evaluations for biological safety of ZnONPs are objectively reviewed in this paper. The discussions addressed in this Review not only have theoretical significance but also are conducive to the development of food safety, nutrition, and human health. The summarized knowledge and future perspectives outlined here are expected to promote and guide new research toward developing and optimizing the application of ZnONPs as a novel class of antifungal agents to help improve food quality as well as food safety in the near future.

Zinc Oxide Nanoparticle as a Novel Class of Antifungal Agents: Current Advances and Future Perspectives.

The study of fungal species evolved radically with the development of molecular techniques and produced new evidence to understand specific fungal mechanisms such as the production of toxic secondary metabolites. Taking advantage of these technologies to improve food safety, the molecular study of toxinogenic species can help elucidate the mechanisms underlying toxin production and enable the development of new effective strategies to control fungal toxicity. Numerous studies have been made on genes involved in aflatoxin B1 (AFB1) production, one of the most hazardous carcinogenic toxins for humans and animals. The current review presents the roles of these different genes and their possible impact on AFB1 production. We focus on the toxinogenic strains Aspergillus flavus and A. parasiticus, primary contaminants and major producers of AFB1 in crops. However, genetic reports on A. nidulans are also included because of the capacity of this fungus to produce sterigmatocystin, the penultimate stable metabolite during AFB1 production. The aim of this review is to provide a general overview of the AFB1 enzymatic biosynthesis pathway and its link with the genes belonging to the AFB1 cluster. It also aims to illustrate the role of global environmental factors on aflatoxin production and the recent data that demonstrate an interconnection between genes regulated by these environmental signals and aflatoxin biosynthetic pathway.

Aflatoxin Biosynthesis and Genetic Regulation: A Review.

Poly(lactic) acid (PLA) and starch bilayer films, containing cinnamaldehyde, obtained by compression moulding

Cinnamaldehyde (CIN) is a promising natural preservative and generally recognized as safe for commodities as well as consumers. In this work, the antifungal effects of CIN on Aspergillus flavus were evaluated both in solid and in liquid culture conditions. Our results indicated that CIN effectively inhibited radial growth, spore production, mycelium formation, and aflatoxin B1 biosynthesis by A. flavus in a dose-dependent manner. At the concentration of 104 mg L(-1), CIN exposure was able to completely inhibit fungal growth as well as aflatoxin B1 production. Furthermore, the inhibitory activities of CIN were closely connected with the treatment period and the tested fungal species. Compared with the control strains, CIN dose dependently changed the morphology and ultrastructure of mycelium in different degree. Especially, the reduction of hydrogen peroxide was considered to follow the destruction of mitochondrial. Meanwhile, CIN significantly cut the levels of lipid peroxidation and reduced glutathione. The activity of total superoxide dismutase was significantly inhibited after CIN treatment at the end of incubation, whereas the activities of catalase and glutathione peroxidase were opposite. These results indicated that the inhibitory effect of CIN could attribute to oxidative stress alleviation possibly induced by modifications of cellular structure as well as redox status.

Yang Liu

Papers

Cinnamaldehyde inhibits fungal growth and aflatoxin B1 biosynthesis by modulating the oxidative stress response of Aspergillus flavus

In vitro anti-aflatoxigenic effect and mode of action of cinnamaldehyde against aflatoxin B1