抗原变异可使得多种致病微生物易于逃避宿主免疫应答。表达在感染红细胞表面的恶性疟原虫红细胞表面蛋白1（PfPMP1）与感染红细胞、内皮细胞、树突状细胞以及胎盘的单个或多个受体作用，在黏附及免疫逃避中起关键的作用。每个单倍体基因组var基因家族编码约60种成员，通过启动转录不同的var基因变异体为抗原变异提供了分子基础。

疟原虫var基因转换速率变化导致抗原变异[英]／Paul H, Robert P, Christodoulou Z, et al//Proc Natl Acad Sci U S A

A fast-Fourier-transform method of topography and interferometry is proposed. By computer processing of a noncontour type of fringe pattern, automatic discrimination is achieved between elevation and depression of the object or wave-front form, which has not been possible by the fringe-contour-generation techniques. The method has advantages over moire topography and conventional fringe-contour interferometry in both accuracy and sensitivity. Unlike fringe-scanning techniques, the method is easy to apply because it uses no moving components.

Fourier-transform method of fringe-pattern analysis for computer-based topography and interferometry

Stochastic thermodynamics as reviewed here systematically provides a framework for extending the notions of classical thermodynamics such as work, heat and entropy production to the level of individual trajectories of well-defined non-equilibrium ensembles. It applies whenever a non-equilibrium process is still coupled to one (or several) heat bath(s) of constant temperature. Paradigmatic systems are single colloidal particles in time-dependent laser traps, polymers in external flow, enzymes and molecular motors in single molecule assays, small biochemical networks and thermoelectric devices involving single electron transport. For such systems, a first-law like energy balance can be identified along fluctuating trajectories. For a basic Markovian dynamics implemented either on the continuum level with Langevin equations or on a discrete set of states as a master equation, thermodynamic consistency imposes a local-detailed balance constraint on noise and rates, respectively. Various integral and detailed fluctuation theorems, which are derived here in a unifying approach from one master theorem, constrain the probability distributions for work, heat and entropy production depending on the nature of the system and the choice of non-equilibrium conditions. For non-equilibrium steady states, particularly strong results hold like a generalized fluctuation–dissipation theorem involving entropy production. Ramifications and applications of these concepts include optimal driving between specified states in finite time, the role of measurement-based feedback processes and the relation between dissipation and irreversibility. Efficiency and, in particular, efficiency at maximum power can be discussed systematically beyond the linear response regime for two classes of molecular machines, isothermal ones such as molecular motors, and heat engines such as thermoelectric devices, using a common framework based on a cycle decomposition of entropy production. (Some figures may appear in colour only in the online journal) This article was invited by Erwin Frey.

/pdf/stochastic-thermodynamics-fluctuation-theorems-and-molecular-2epwyvmpf0.pdf

Stochastic thermodynamics, fluctuation theorems and molecular machines

Thank you for reading principles of optics electromagnetic theory of propagation interference and diffraction of light. As you may know, people have search hundreds times for their favorite novels like this principles of optics electromagnetic theory of propagation interference and diffraction of light, but end up in harmful downloads. Rather than enjoying a good book with a cup of coffee in the afternoon, instead they are facing with some infectious bugs inside their computer.

Principles Of Optics Electromagnetic Theory Of Propagation Interference And Diffraction Of Light

: The unpolarized absorption and circular dichroism spectra of the fundamental vibrational transitions of the chiral molecule, 4-methyl-2-oxetanone, are calculated ab initio. Harmonic force fields are obtained using Density Functional Theory (DFT), MP2, and SCF methodologies and a 5S4P2D/3S2P (TZ2P) basis set. DFT calculations use the Local Spin Density Approximation (LSDA), BLYP, and Becke3LYP (B3LYP) density functionals. Mid-IR spectra predicted using LSDA, BLYP, and B3LYP force fields are of significantly different quality, the B3LYP force field yielding spectra in clearly superior, and overall excellent, agreement with experiment. The MP2 force field yields spectra in slightly worse agreement with experiment than the B3LYP force field. The SCF force field yields spectra in poor agreement with experiment.The basis set dependence of B3LYP force fields is also explored: the 6-31G* and TZ2P basis sets give very similar results while the 3-21G basis set yields spectra in substantially worse agreements with experiment. jg

Ab initio calculation of vibrational absorption and circular dichroism spectra using density functional force fields

Microfluidic mixing is one of the important topics with a various application for example biomedicine, chemical engineering, etc. We present a method for imaging and quantifying microfluidic mixing using quantitative phase imaging method.

Quantitative Phase Imaging of Fluid Mixing in Microfluid Chips

Disclosed herein are a multifunctional optical element and method using multiple light scattering. An optical control method using multiple light scattering may include the steps of splitting coherent light into a signal beam and a reference beam, controlling the wavefront of the signal beam, forming an interference pattern by making the signal beam having the controlled wavefront and the reference beam incident on photorefractive materials, recording the interference pattern on the photorefractive materials, reconstructing the signal beam having the controlled wavefront by the interference pattern by radiating the reference beam to the photorefractive materials on which the interference pattern has been recorded again, and controlling the properties of light passing through complex media based on multiple light scattering generated by the complex media as the reconstructed signal beam is incident on the complex media.

Multifunctional optical element and method using multiple light scattering

eISSN 2093-6338 there is no de nite marker to distinguish MDS from other hematologic diseases based on the patient’s peripheral blood. Findings of dysplastic features from peripheral blood are limited. Large or abnormally granulated platelets, pseudo–Pelger-Huët anomaly and hypogranular cytoplasm in neutrophils, basophilic stippling, and poikilocytosis in red blood cells (RBCs) are features that can be observed in a peripheral blood smear using light microscopy [1]. However, these features are not unique to MDS and are not observed frequently. Bone marrow examination must therefore be performed to diagnose MDS. In this study, we employed optical diffraction tomography (ODT) to perform three-dimensional (3-D) imaging of RBCs from a patient with MDS. Based on laser interferometry, ODT reconstructs the 3-D refractive index (RI) distributions of samples without a labeling procedure [2]. The RI tomograms of individual RBCs provide information about the morphological, biochemical, and mechanical parameters at the individual cell level [3]. Conventionally, 3-D images of RBCs have been obtained using scanning electron microscopy (SEM) [4]. However, SEM has limitations in its timeINTRODUCTION

/pdf/unique-red-blood-cell-morphology-detected-in-a-patient-with-1mk9b0iqph.pdf

Unique Red Blood Cell Morphology Detected in a Patient with Myelodysplastic Syndrome by Three-dimensional Refractive Index Tomography

Antimicrobial susceptibility test (AST) is widely used to provide the minimum inhibitory concentration of bacteria, and crucial to provide appropriate uses of antibiotics and to address the issue of drug-resistance bacteria. However, ASTs require the time-consuming incubation about 16-20 h for the visual determination of the growth of bacterial colonies, which has been a major obstacle to on-site applications of ASTs. In this study, we propose a rapid and non-invasive method based on laser speckles to evaluate the bacterial growth movements in real time, thus reducing the time for the agar dilution method. With a simple configuration compatible with conventional agar plates, the analysis of laser speckle from samples enables the early detection of the presence of growth as well as its detailed history of the colony-forming movement on agar plates. Using the samples prepared through the same procedure as the agar dilution method, we obtained the AST results at least 4-8 hours earlier than the conventional method without compromising the accuracy. This technique does not require for the use of exogenous agents, but works for most bacteria regardless of their species. Furthermore, the distinctive responses of several species to microbial agents were revealed through the present technique supporting a comprehensive analysis of the effect of the antibiotics. The findings suggest that this new method could be a useful tool for rapid, simple, and low-cost ASTs in addition to providing the historical information of the bacterial growth on agar plates.

https://www.biorxiv.org/content/biorxiv/early/2018/11/29/481184.full.pdf

Real-time monitoring of bacterial growth and fast antimicrobial susceptibility tests exploiting multiple light scattering

Optical diffraction tomography (ODT) produces three dimensional distribution of refractive index (RI) by measuring scattering fields at various angles. Although the distribution of RI index is highly informative, due to the missing cone problem stemming from the limited-angle acquisition of holograms, reconstructions have very poor resolution along axial direction compared to the horizontal imaging plane. To solve this issue, here we present a novel unsupervised deep learning framework, which learns the probability distribution of missing projection views through optimal transport driven cycleGAN. Experimental results show that missing cone artifact in ODT can be significantly resolved by the proposed method.

YongKeun Park

Papers

Quantitative Phase Imaging of Fluid Mixing in Microfluid Chips

Multifunctional optical element and method using multiple light scattering

Unique Red Blood Cell Morphology Detected in a Patient with Myelodysplastic Syndrome by Three-dimensional Refractive Index Tomography

Real-time monitoring of bacterial growth and fast antimicrobial susceptibility tests exploiting multiple light scattering

Unsupervised Missing Cone Deep Learning in Optical Diffraction Tomography.