抗原变异可使得多种致病微生物易于逃避宿主免疫应答。表达在感染红细胞表面的恶性疟原虫红细胞表面蛋白1（PfPMP1）与感染红细胞、内皮细胞、树突状细胞以及胎盘的单个或多个受体作用，在黏附及免疫逃避中起关键的作用。每个单倍体基因组var基因家族编码约60种成员，通过启动转录不同的var基因变异体为抗原变异提供了分子基础。

疟原虫var基因转换速率变化导致抗原变异[英]／Paul H, Robert P, Christodoulou Z, et al//Proc Natl Acad Sci U S A

A fast-Fourier-transform method of topography and interferometry is proposed. By computer processing of a noncontour type of fringe pattern, automatic discrimination is achieved between elevation and depression of the object or wave-front form, which has not been possible by the fringe-contour-generation techniques. The method has advantages over moire topography and conventional fringe-contour interferometry in both accuracy and sensitivity. Unlike fringe-scanning techniques, the method is easy to apply because it uses no moving components.

Fourier-transform method of fringe-pattern analysis for computer-based topography and interferometry

Stochastic thermodynamics as reviewed here systematically provides a framework for extending the notions of classical thermodynamics such as work, heat and entropy production to the level of individual trajectories of well-defined non-equilibrium ensembles. It applies whenever a non-equilibrium process is still coupled to one (or several) heat bath(s) of constant temperature. Paradigmatic systems are single colloidal particles in time-dependent laser traps, polymers in external flow, enzymes and molecular motors in single molecule assays, small biochemical networks and thermoelectric devices involving single electron transport. For such systems, a first-law like energy balance can be identified along fluctuating trajectories. For a basic Markovian dynamics implemented either on the continuum level with Langevin equations or on a discrete set of states as a master equation, thermodynamic consistency imposes a local-detailed balance constraint on noise and rates, respectively. Various integral and detailed fluctuation theorems, which are derived here in a unifying approach from one master theorem, constrain the probability distributions for work, heat and entropy production depending on the nature of the system and the choice of non-equilibrium conditions. For non-equilibrium steady states, particularly strong results hold like a generalized fluctuation–dissipation theorem involving entropy production. Ramifications and applications of these concepts include optimal driving between specified states in finite time, the role of measurement-based feedback processes and the relation between dissipation and irreversibility. Efficiency and, in particular, efficiency at maximum power can be discussed systematically beyond the linear response regime for two classes of molecular machines, isothermal ones such as molecular motors, and heat engines such as thermoelectric devices, using a common framework based on a cycle decomposition of entropy production. (Some figures may appear in colour only in the online journal) This article was invited by Erwin Frey.

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Stochastic thermodynamics, fluctuation theorems and molecular machines

Thank you for reading principles of optics electromagnetic theory of propagation interference and diffraction of light. As you may know, people have search hundreds times for their favorite novels like this principles of optics electromagnetic theory of propagation interference and diffraction of light, but end up in harmful downloads. Rather than enjoying a good book with a cup of coffee in the afternoon, instead they are facing with some infectious bugs inside their computer.

Principles Of Optics Electromagnetic Theory Of Propagation Interference And Diffraction Of Light

: The unpolarized absorption and circular dichroism spectra of the fundamental vibrational transitions of the chiral molecule, 4-methyl-2-oxetanone, are calculated ab initio. Harmonic force fields are obtained using Density Functional Theory (DFT), MP2, and SCF methodologies and a 5S4P2D/3S2P (TZ2P) basis set. DFT calculations use the Local Spin Density Approximation (LSDA), BLYP, and Becke3LYP (B3LYP) density functionals. Mid-IR spectra predicted using LSDA, BLYP, and B3LYP force fields are of significantly different quality, the B3LYP force field yielding spectra in clearly superior, and overall excellent, agreement with experiment. The MP2 force field yields spectra in slightly worse agreement with experiment than the B3LYP force field. The SCF force field yields spectra in poor agreement with experiment.The basis set dependence of B3LYP force fields is also explored: the 6-31G* and TZ2P basis sets give very similar results while the 3-21G basis set yields spectra in substantially worse agreements with experiment. jg

Ab initio calculation of vibrational absorption and circular dichroism spectra using density functional force fields

Accurate and rapid evaluation of dynamic immune status is critical to determine therapeutic modalities for sepsis patients, which is impeded by the limitations of conventional diagnostic tools. Here, we employ refractive index tomography to quantitatively assess the immune status of human monocytes in a label-free manner. Measurement of refractive index tomograms enabled quantifications of three-dimensional morphological parameters, which revealed a clear increment in lipid droplets content and intracellular inhomogeneities as the septic stage progresses. We leveraged these observations to engineer a deep-learning-based algorithm that predicts the immune status of monocytes, showing over 99 % blind test accuracy. 

Machine-learning-based analysis of refractive index tomograms to predict the immune status of individual human monocytes (Conference Presentation)

The refractive index (RI) of cells and tissues is crucial in pathophysiology as a noninvasive and quantitative imaging contrast. Although its measurements have been demonstrated using three-dimensional quantitative phase imaging methods, these methods often require bulky interferometric setups or multiple measurements, which limits the measurement sensitivity and speed. Here, we present a single-shot RI imaging method that visualizes the RI of the in-focus region of a sample. By exploiting spectral multiplexing and optical transfer function engineering, three color-coded intensity images of a sample with three optimized illuminations were simultaneously obtained in a single-shot measurement. The measured intensity images were then deconvoluted to obtain the RI image of the in-focus slice of the sample. As a proof of concept, a setup was built using Fresnel lenses and a liquid-crystal display. For validation purposes, we measured microspheres of known RI and cross-validated the results with simulated results. Various static and highly dynamic biological cells were imaged to demonstrate that the proposed method can conduct single-shot RI slice imaging of biological samples with subcellular resolution.

Single-shot refractive index slice imaging using spectrally multiplexed optical transfer function reshaping.

Optical diffraction tomography (ODT) enables the three-dimensional (3D) refractive index (RI) reconstruction. However, when the RI difference between a sample and a medium increases, the effects of light scattering become significant, preventing the acquisition of high-quality and accurate RI reconstructions. Herein, we present a method for high-fidelity ODT by introducing non-toxic RI matching media. Optimally reducing the RI contrast enhances the fidelity and accuracy of 3D RI reconstruction, enabling visualization of the morphology and intra-organization of live biological samples without producing toxic effects. We validate our method using various biological organisms, including C. albicans and C. elegans.

High-fidelity optical diffraction tomography of live organisms using iodixanol refractive index matching.

Herein, we propose an experimental scheme to determine the strength of the Heisenberg exchange interaction in ultrathin magnetic films. In this scheme, the chirality transition between the Bloch- and Néel-type domain walls is analyzed under an in-plane magnetic field. Subsequently, the exchange stiffness constant is estimated based on the strength of the magnetic field for chirality transitions, as proposed by an analytical theory pertaining to chirality transitions, and confirmed based on micromagnetic prediction. Using the magneto-optical Kerr effect with high sensitivity on surface atomic layers, the present scheme is applicable to ultrathin magnetic films down to a few atomic layers, whose sensitivity is well below the lowest sensitivity limit of conventional measurement schemes. Hence, the present scheme is useful for extending the experimental range to investigate the exchange stiffness of few-atomic-layer-thick magnetic films.

Exchange-stiffness measurement scheme based on domain-wall chirality transition

The dielectric tensor is a physical quantity which characterizes birefringent materials with principal refractive indices and orientations of optic axes. Recently, three-dimensional dielectric tensor distribution was directly measured using dielectric tensor tomography (DTT). However, since the original DTT uses two cameras to acquire polarization-sensitive fields, position disagreement between the two fields deteriorates reconstruction quality. Here, we present multiplexed DTT using only one camera. To avoid the position disagreement, we exploit holographic multiplexing, interfering two orthogonally polarized reference beams with a sample beam on the camera. We validate the present method via measurement of anisotropic structures in liquid crystal particles. 

YongKeun Park

Papers

Machine-learning-based analysis of refractive index tomograms to predict the immune status of individual human monocytes (Conference Presentation)

Single-shot refractive index slice imaging using spectrally multiplexed optical transfer function reshaping.

High-fidelity optical diffraction tomography of live organisms using iodixanol refractive index matching.

Exchange-stiffness measurement scheme based on domain-wall chirality transition

Multiplexed dielectric tensor tomography for cost-effective and robust dielectric tensor reconstruction (Conference Presentation)