Yu-Hsuan Wu

TL;DR: Evidence is reported for nsEP‐induced permeabilization of mitochondrial membranes in living cells using three different methods with fluorescence indicators and it is shown that multiple nsEPs cause an increase of the inner mitochondrial membrane permeability and an associated loss of mitochondrial membrane potential.

TL;DR: It is shown here, with results from both molecular dynamics simulations and experiments with living cells, that the oxidation of membrane components enhances the susceptibility of the membrane to electropermeabilization.

TL;DR: In this paper, a single-stage resonant network is proposed to produce high voltage pulses with half-maximum widths as short as 2.5 ns and amplitudes as high as 5 kV.

TL;DR: Plasma membrane electropermeabilization is characterized by exposing Jurkat T lymphoblasts to pulsed electric fields less than 10ns long (including single pulse exposures), and by monitoring the resulting osmotically driven cell swelling as a function of pulse number and pulse repetition rate.

TL;DR: This live-cell method for measuring a nanosecond pulsed electric field distribution provides an operationally meaningful calibration of electrode designs for biological applications and permits visualization of the relative sensitivities of different cell types to nanoelectropulse stimulation.