Yves Lachance

TL;DR: A second type of cDNA clone (arbitrarily designated type II) encoding 3βHSD is reported after screening of a human adrenal λgt22A library, which predicts a protein of 371 amino acids with a calculated molecular mass of 41,921 daltons.

TL;DR: Polyclonal antibodies raised against 3β-hydroxysteroid dehydrogenase isolated from human placenta were used to screen a λgt11 expression cDNA library from the same tissue, deducing the protein deduced from cDNA sequences contains 372 amino acids.

TL;DR: Since E2DH is the enzyme required for the formation of 17 beta-estradiol, the availability of the cDNA encoding the enzyme should permit a detailed investigation of the factors regulating the expression and activity of this crucial enzyme, in both normal and malignant tissues, especially breast cancer.

TL;DR: The complete exon and intron sequences of the two genes as well as their 5′ and 3′-flanking regions are determined and provide the basis for a better understanding of the molecular mechanisms involved in 17β-HSD deficiency and peripheral sex steroid metabolism.

TL;DR: In this article, the authors described the isolation and complete sequence of the corresponding type II 3 beta-HSD gene, which is the form most likely responsible for human 3 Beta-HHSD deficiency.