Showing papers by "Nuffield Orthopaedic Centre published in 1972"

The pathological basis for skeletal scintigraphy.

Abstract: Tumour-cell suspensions of the VX2 carcinoma were injected into the medullary cavity or on to the periosteal surface of the ilia or tibiae of New Zealand white rabbits. A combination of bone destruction and new bone formation, similar to the autopsy material, was seen. There were at least two mechanisms for the new bone production. Initially, intramembranous ossification was seen in the fibrous stroma surrounding the tumour. Once the cortex was involved and cortical hone destruction had occurred, large amounts of woven bone resembling fracture callus were laid down. The new bone had a markedly increased avidity for boneseeking isotopes, indicating why skeletal scintigraphy was useful. A further twenty rabbits, in whose ilia the VX2 carcinoma was growing, were treated by local irradiation. When treatment was successful the tumour was destroyed, the production of new bone ceased, and the lesion lost its increased avidity for bone-seeking isotopes, indicating that skeletal scintigraphy could be used to assess the response of skeletal metastases to therapy. During the past few years skeletal scintigraphy has become established as a useful method of examining the skeleton, particularly for the early detection of skeletal metastases (Sklaroff and Charkes 1968 ; Galasko 1972) and for the assessment of response of these lesions to treatment (Galasko and Doyle 1972). The underlying pathological changes which allow the use of isotopes for the investigation of skeletal metastases are not well understood. In this study the reaction of bone to metastatic cancer was investigated, both in man and in the experimental animal, in an attempt to understand the pathological basis of skeletal scintigraphy.

Intracellular collagen in experimental arthritis in rats

Abstract: 1. Experimental arthritis was induced in rats by the intradermal injection of modified Freund's adjuvant. 2. The granulation tissue occurring in and around the joints was examined with the electron microscope. 3. Intracellular collagen was demonstrated in many of the cells. 4. Collagen formation by these cells was studied by autoradiographic techniques using tritiated proline as a label. 5. The proline turnover was rapid, as most of the labelled proline had become extracellular one hour after its injection. 6. It was concluded that the collagen was present within the cells as a result of phagocytosis despite the fact that the cells had the electron microscopic features of fibroblasts.