Showing papers by "Tata Memorial Hospital published in 1987"
TL;DR: Bilateral full thickness naso-labial flaps have been used successfully in three patients to give long-term relief of the severe trismus caused by oral submucous fibrosis and the postoperative rehabilitation compared favourably with other methods.
60 citations
TL;DR: Residual cancer was found in 58% of the cases, the morbidity was low, and there was no operative mortality of the operation.
Abstract: We have reviewed the charts of 149 patients who underwent completion thyroidectomies for cancer of the thyroid. The purpose of the study was to evaluate extent of residual disease in the thyroid, and morbidity and mortality of the operation. Residual cancer was found in 58% of the cases, the morbidity was low, and there was no operative mortality.
25 citations
TL;DR: A retrospective study of 20 cases of biopsy-proven solitary rectal ulcer syndrome seen at a large oncology hospital which highlight the clinicopathologic features is presented.
Abstract: A retrospective study of 20 cases of biopsy-proven solitary rectal ulcer syndrome seen at a large oncology hospital which highlight the clinicopathologic features is presented. Thirty five percent of patients were in their sixth decade and there was a male preponderance of 2.3∶1. All patients presented with anorectal symptoms. Eighty five percent had normal abdominal findings and seventy five percent had positive findings on rectal examinations. Fifty four percent had a diagnosis of carcinoma of the rectum on barium enema studies. On sigmoidoscopy, a solitary ulcer was found in 80 percent; 50 percent had lesions between 6 and 10 cm from the anal verge, and 40 percent had the lesion on the anterior rectal wall. All patients were treated conservatively and 35 percent were disease free at the end of five years, while 30 percent had persisting symptoms.
17 citations
TL;DR: The reaction for metastatic lesions from patients with non-prostatic carcinomas was completely negative indicating the specificity of the test for prostatic origin of metastasis, indicating the potential application of ILM in confirming or excluding the prostaticorigin of invasive tumour in metastatic patients.
15 citations
TL;DR: It is suggested that this differential interaction between Fl-Con A and normal and leukemic granulocytes is a convenient system to study the initial steps in receptor mediated endocytosis of Concanavalin A.
Abstract: Isolated granulocytes from normal individuals and patients suffering from chronic myeloid leukemia (CML) displayed different fluorescent patterns on treatment with fluorescein isothiocyanate concanavalin A (Fl-Con A). The ligand was internalized by 86% of the normal granulocytes, while 80% of the leukemic granulocytes exhibited Fl-Con A localized on the cell periphery. In further experiments, pretreatment of the normal granulocytes with cytochalasin B, iodoacetamide, 2-deoxyglucose and sodium fluoride (but not with sodium azide or dinitrophenol) was found to drastically inhibit internalization of the ligand. However, pretreatment of granulocytes from CML patients with cytochalasin B and 2-deoxyglucose, caused only a little alteration in the pattern of Fl-Con A labelling relative to untreated cells. These results indicate that CML granulocytes are defective in their ability to endocytose Fl-Con A. We suggest that this differential interaction between Fl-Con A and normal and leukemic granulocytes is a convenient system to study the initial steps in receptor mediated endocytosis of Concanavalin A.
14 citations
TL;DR: The study shows promise of an aesthetic flap for covering facial defects and more clinical work and long term follow-up are in progress.
9 citations
TL;DR: The mechanism of restoration of drug sensitivity by nitroxazepine hydrochloride in adriamycin-resistant P388 cells could be due to an enhanced intracellular accumulation of adri amycin.
Abstract: Attempts were made to reverse the acquired resistance of P388 murine leukemia utilizing non-toxic concentration of nitroxazepine hydrochloride, an antidepressant. The effect of nitroxazepine hydrochloride on the intracelluar accumulation of adriamycin and the inhibition of DNA synthesis were studied in these cells. The survival of mice bearing adriamycin-resistant P388 murine leukemia cells treated in vitro with nitroxazepine hydrochloride, adriamycin and a combination of the two drugs was also investigated. The results show that treatment of these cells with nitroxazepine hydrochloride significantly enhanced (1) the intracellular accumulation of adriamycin, (2) inhibition of DNA biosynthesis, and (3) the survival of mice transplanted with adriamycin-resistant P388 murine leukemia cells treated in vitro with a combination of nitroxazepine hydrochloride and adriamycin. The mechanism of restoration of drug sensitivity by nitroxazepine hydrochloride in adriamycin-resistant P388 cells could be due to an enhan...
6 citations
01 Jan 1987
TL;DR: The blastogenic response to PHA and IL-2 production by lymphocytes of HD patients was significantly lower than that of normal lymphocytes and the Tac antigen expression was found to be lower in PHA- Stimulated but not alloantigen-stimulated lymphocytes from the HD patients.
Abstract: Phytohemagglutinin (PHA)- induced Interleukin-2 (IL-2) production by peripheral blood mononuclear cells was studied in 28 untreated patients with Hodgkin Disease (HD). A group of 28 patients were also investigated for the expression of Tac antigen in the resting stage of lymphocytes and after activation with PHA and mixed leukocyte culture (using anti-Tac monoclonal antibody). The blastogenic response to PHA and IL-2 production by lymphocytes of HD patients was significantly lower than that of normal lymphocytes. Production of IL-2 appeared to be severely affected in 14 of 28 HD patients who also showed PHA response less than the normal range. The Tac antigen expression was found to be lower in PHA-stimulated but not alloantigen-stimulated lymphocytes from the HD patients. No correlation was observed between the levels of IL-2 production, Tac antigen expression, and blastogenic response to PHA or allogeneic cells and the stage of disease when tested in the same patients.
6 citations
TL;DR: Granulocytes from normal human donors and patients suffering from chronic myeloid leukemia (CML) were externally labeled with125Iodine, using the Iodogen method, and spot 4 is expressed more intensely in the normal granulocyte while spots 3 a and 3 b are mainly expressed on the leukemic granulocytes.
Abstract: In this work granulocytes from normal human donors and patients suffering from chronic myeloid leukemia (CML) were externally labeled with125Iodine, using the Iodogen method.125Iodine labeled Concanavalin A binding proteins (CBP) and detergent-resistant proteins (DRP) were isolated from the cell lysates and characterized by one- and two-dimensional sodium dodecyl sulfate polyacrylamide gel electrophoresis (1D- and 2D-SDS-PAGE). Autoradiographs of the 2D-gels of DRP show seven proteins with Mr 118,000 (spot 1 a), Mr 112,000 (spot 1 b), Mr 78,000–85,000 (spot 2), Mr 85,000 (spot 4), Mr 52,000 (spot 3, 3 a and 3 b). Of this set, spot 1 b, 2 and 4 are also present in the autoradiographs of 2D-gels of CBP and, hence, may be considered to be transmembrane components. Spot 4 is expressed more intensely in the normal granulocytes while spots 3 a and 3 b are mainly expressed on the leukemic granulocytes.
5 citations
TL;DR: In this article, the authors have shown that the outcome in chronic myeloid leukemia so far has been poor and there is urgent need for expansion of bone marrow transplantation (BMT) facilities in India.
Abstract: Beginning in early 1983, bone marrow transplantation (BMT) facilities in India have undergone slow but steady growth. With about 50% disease-free survival in acute myeloid leukaemia and aplastic anaemia and 90% survival in thalassemia major, the results are encouraging. However, the outcome in chronic myeloid leukemia so far has been poor. There is urgent need for expansion of BMT facilities in India.
5 citations
TL;DR: The high specificity (100%) and sensitivity (92%) of MoAb GM 58/8 for myeloblastic leukemia is unmatched by almost all previously described myeloid MoAb and proves its usefulness as a single diagnostic reagent for AML and myelobastic crisis in CGL.
Abstract: Monoclonal antibody (MoAb) GM 58/8 was earlier reported to be directed against an antigen expressed by myeloid progenitors (CFU-GM), myeloid precursors, granulocytes, and monocytes. Immunophenotyping of 216 cases of acute leukemia [acute myeloblastic leukemia (AML) = 147 and acute lymphoblastic leukemia (ALL) = 69] and 18 cases of chronic granulocytic leukemia in blast crisis (CGLBC) with this antibody showed that GM 58/8 reacted with 92% of AML cases (M1-M5) and 100% of myeloblastic crisis in CGL cases. All cases of ALL, lymphoblastic crisis in CGL, erythroleukemia, and erythroblastic crisis in CGL were unreactive with GM 58/8. The antibody revealed the myeloid phenotype in an additional 15 cases of otherwise unclassifiable acute leukemia and six cases of CGLBC. Eleven cases of acute "mixed lineage" leukemia were also diagnosed with the help of GM 58/8. The high specificity (100%) and sensitivity (92%) of MoAb GM 58/8 for myeloblastic leukemia is unmatched by almost all previously described myeloid MoAb and proves its usefulness as a single diagnostic reagent for AML and myeloblastic crisis in CGL.
01 Jan 1987
TL;DR: In this article, the authors present an overview of the mechanistic aspects of the aqueous two-phase polymer system, describing the excellence of this system in the simplest possible way to make it accessible to most laboratories interested in cell separation.
Abstract: The main objective of this article is to present an overview of the mechanistic aspects of the aqueous two-phase polymer system, describing the excellence of this system in the simplest possible way to make it accessible to most laboratories interested in cell separation. The construction, operation, and application of a manually operable countercurrent distribution (CCD) apparatus are discussed.
TL;DR: The dose-dependent increase in the inhibition of these tumor cells by vitamin A is characterized by undifferentiated morphology showing uniformity in the given tumor cell population associated with a total lack of differentiation.
Abstract: The effect of vitamin A compounds on the DNA biosynthesis inhibition of murine ascites tumor models such as P388 lymphocytic leukemia, L1210 lymphoid leukemia, sarcoma 180 and Ehrlich ascites carcinoma was found to be concentration- and time-dependent. The results of the experiments carried out in vitro for 4 h measured by the extent of 3H-thymidine incorporation into the DNA of these tumor cells permit us to conclude that (a) at the given concentration and time, the metabolic role of vitamin A compounds influences the regulation of processes leading to DNA biosynthesis inhibition; (b) the effect of vitamin A is uniform irrespective of tumor cells heterogeneity; (c) vitamin A does not affect transport of thymidine, and (d) the dose-dependent increase in the inhibition of these tumor cells by vitamin A is characterized by undifferentiated morphology showing uniformity in the given tumor cell population associated with a total lack of differentiation.