Techno India

About: Techno India is a based out in . It is known for research contribution in the topics: Computer science & Cloud computing. The organization has 4724 authors who have published 4005 publications receiving 34112 citations.

Testing and Evaluation of Performance and Emissions Characteristics of Water- Biodiesel Aspirated Research Engine

Abstract: This work aims to investigate the effect of the addition of water to corn oil biodiesel (BD) (COBD) in a diesel engine to view its effect on performance and emission characteristics. Two fuel samples were prepared using a novel surfactant Span 80 (MilliporeSigma, Burlington, MA), with different concentrations (94 % corn oil + 4 % water + 2 % Span 80 (COBDE1) and 90 % corn oil + 8 % water + 2 % Span 80 (COBDE2)). Experimental results showed that the water samples of COBD had higher brake thermal efficiency and lower brake-specific fuel consumption than that of neat COBD at all engine brake power. In addition, smoke and nitrogen oxide emissions are reduced by 4–11 % and 6–10 % using corn oil–water samples compared to neat COBD. Furthermore, the introduction of COBD-water emulsions in a diesel engine reduces the unburned hydrocarbon and carbon monoxide emissions considerably.

Alpha-linolenic acid stabilizes HIF-1 α and downregulates FASN to promote mitochondrial apoptosis for mammary gland chemoprevention

Abstract: // Subhadeep Roy 1 , Atul Kumar Rawat 2 , Shreesh Raj Sammi 3 , Uma Devi 4 , Manjari Singh 1 , Swetlana Gautam 1 , Rajnish Kumar Yadav 1 , Jitendra Kumar Rawat 1 , Lakhveer Singh 1 , Mohd. Nazam Ansari 5 , Abdulaziz S. Saeedan 5 , Rakesh Pandey 3 , Dinesh Kumar 2 and Gaurav Kaithwas 1 1 Department of Pharmaceutical Sciences, Babasaheb Bhimrao Ambedkar University, Lucknow (UP), India 2 Central for Biomedical Research, Sanjay Gandhi Post Graduate Institute of Medical Sciences Campus, Lucknow (UP), India 3 Department of Microbial Technology and Nematology, CSIR-Central Institute of Medicinal and Aromatic Plants, Lucknow (UP), India 4 Department of Pharmaceutical Sciences, Faculty of Health and Medical Sciences, Sam Higginbottom Institute of Agricultural Sciences and Technology, Allahabad (UP), India 5 Department of Pharmacology, College of Pharmacy, Prince Sattam Bin Abdulaziz University, Al-Kharj, KSA Correspondence to: Gaurav Kaithwas, email: gauravpharm@hotmail.com Keywords: alpha linolenic acid, apoptosis, polyunsaturated fatty acid, hypoxia, fatty acid synthase Received: February 06, 2017 Accepted: June 12, 2017 Published: July 25, 2017 ABSTRACT Alpha linolenic acid is an essential polyunsaturated fatty acid and is reported to have the anti-cancer potential with no defined hypothesis or mechanism/s. Henceforth present study was in-quested to validate the effect of alpha linolenic acid on mitochondrial apoptosis, hypoxic microenvironment and de novo fatty acid synthesis using in-vitro and in-vivo studies. The IC 50 value of alpha linolenic acid was recorded to be 17.55μM against ER+MCF-7 cells. Treatment with alpha linolenic acid was evident for the presence of early and late apoptotic signals along with mitochondrial depolarization, when studied through acridine orange/ethidium bromide and JC-1 staining. Alpha linolenic acid arrested the cell cycle in G2/M phase. Subsequently, the in-vivo efficacy was examined against 7, 12-dimethylbenz anthracene induced carcinogenesis. Treatment with alpha linolenic acid demarcated significant effect upon the cellular proliferation as evidenced through decreased in alveolar bud count, restoration of the histopathological architecture and loss of tumor micro vessels. Alpha linolenic acid restored the metabolic changes to normal when scrutinized through 1 H NMR studies. The immunoblotting and qRT-PCR studies revealed participation of mitochondrial mediated death apoptosis pathway and curtailment of hypoxic microenvironment after treatment with alpha linolenic acid. With all above, it was concluded that alpha linolenic acid mediates mitochondrial apoptosis, curtails hypoxic microenvironment along with inhibition of de novo fatty acid synthesis to impart anticancer effects.