Showing papers by "Yonsei University published in 1969"

Studies On Lactic Dehydrogenase Activities In Developmental Stages Of Fertilized Eggs Of Ascaris Lumbricoides

Abstract: The lactic dehydrogenase activities of Ascaris lumbricoides have been assayed according to the developmental course of the fertilized eggs compared with the unfertilized eggs. Experimental procedures were modified that of Wroblewski and La Due's method with use of diphosphopyridine nucleotide reduced form(DPNH). Sodium pyruvate and lactic dehydrogenase, the specific activities were expressed as DPNH unit per mg protein by method of Kornberg's. The experimental results are as follaws: 1. Lactic dehydrogenase activity in fertilized eggs of Ascaris are significantly increased from morula stage and its optimal pH is in 3.4 degrees. While any activities were not observed in unicell stage of fertilized eggs and unfertilized eggs. 2. Water soluble protein of fertilized eggs of Ascaris were varied in their amount during their developmental process. The amont of protein in tadpole stage eggs have increased to 1.3 times more than that of cleavage stage eggs. 3. However the amounts of water soluble protein in unfertilized eggs are revealed to one-third less than that of fertlized eggs. 4. The specific activity of lactic dehydrogenase in tadpole stage eggs are decreased to one third less than those of morula stage eggs during development, the stage is considered to be as a growth criteria in developmental process, as on this point the activity of aerobic cytochrome coxidase and cyclophorase begin to increase respectively.

Virulence Of Entamoeba Histolytica According To The Strains In Korea: III. Amebicial Response Of Antiamoebic Agents On Several Strains Of Entamoeba Histolytica In Vitro.

Abstract: The amebicidal activity of traditional anitiamoebic drug (emetine, carbasone, diodoquin, chloroquine, atabrine, chloramphenicol and tertracycline) and newly appeared chemicals(niridazole, metronidazole and No. 8603 substance) were assayed by in vitro experiment using five strains of human originated E, histolytica. The variety of amebicidal activity of drugs by the strains were discussed. 1)Ranges of amoebicidal activity of traditional antiamoebic drugs kept almost similar titers of previous reports at the concentration; 1:5,000 to 1:20,000 with emetine hydrochloride, 1:10.000 to 1:20,000 with carbarsone, 1:8,000 to 1:16,000 with diodoquin, 1:50,000 with chloroquine, 1:1,000 to 1: 4,000 with atabrine,1:1,000 to 1:2,000 with chloramphencol and 1:5,000 to 1:8,000 with tetracycline. The newly appeared chemicals showed higher amebicidal titres at the concentration; 1:500,000 to 1:5,000,000 with niridazole, 1:50,000 to 1:100,000 with metronidazol and 1:100,000 to 1:500,000 with No.8603 substance. 2)Emetine, chloramphenicol and No. 8603 substance showed amebicidal activities at lower concentration to intestine originated amebae (YS 14, YS 15 and NAMRU II strain) than to liver originated amebae (YS 24 and YS 25 strain), while carbarsone, chloroquine and metronidazole showed the activity at higher concentrations. Diodoquin showed lower amebicidal titres to trophozoite borne amebae (NAMRU II, YS 24 and YS 25 strain) than to cyst borne amebae(YS 14 and YS 15 strain), but niridazole showed converse results. The concentration of atabrine for amebicidal activity was not constant according to strains of the amoeba, but tetracycline showed almost settled titers.