Showing papers in "China Journal of Modern Medicine in 2015"

Effects of mitochondrial fission inhibitor on the mitochondrial morphology and function after acute spinal cord injury in rats

Abstract: 【Objective】To detect the effect of selective mitochondrial fission inhibitor-Mdivi-1 on mitochondrial morphology, mitochondrial membrane potential and the content of mitochondrial ATP after acute spinal cord injury in rats. 【Methods】72 adult female SD rats, weighing 250~300 g, were randomly divided into 3 groups(n =24):sham operation group(Sham group), acute spinal cord injury group(SCI group), Mdivi-1 pretreatment group(1.20 mg/kg, Mdivi-1 group) using a random number table. The Spinal cord injury model of rats in SCI group and Mdivi-1group were made by Allen's method. In sham group, the rats were exposed, but not hit. In Mdivi-1 group, the rats were given Mdivi-1 15 min before spinal cord injury through the tail vein, but SCI group received the same amount of dimethyl sulfoxide(DMSO). Each group was divided into two sub-groups. The rats were sacrificed at 3 h and 12 h respectively after exposing spinal cord or spinal cord injury, and then the spinal cord T9-11 was removed. Mitochondrial morphology was detected by transmission electron microscopy. Mitochondrial membrane potential was detected by fluorescence microscope and fluorescence spectrophotometer. The content of mitochondrial ATP was detected by fluorescence spectrophotometer.【Results】Compared with Sham group, the number of mitochondria in SCI 3 h group reduced significantly(P 0.01) and cross-sectional area increased significantly(P 0.01), but no significant changes were found in mitochondrial membrane potential and content of mitochondrial ATP; The number of mitochondria in the SCI group at 12 h increased significantly(P 0.01), but cross-sectional area, mitochondrial membrane potential and content of mitochondrial ATP reduced significantly(P 0.01). Compared with the SCI group, the number of mitochondria, cross-sectional area, mitochondrial membrane potential and content of mitochondrial ATP have no significant changes in the Mdivi-1 group at 3 h; However, the number of mitochondria reduced significantly(P 0.01), but cross-sectional, mitochondrial membrane potential and content of mitochondrial ATP increased significantly in the Mdivi-1 group at 12 h(P 0.01). 【Conclusions】Mdivi-1 can inhibit effectively mitochondrial fission and reduce mitochondrial membrane potential, and increase the ATP synthesis in mitochondria, so protect the mitochondrial function after acute spinal cord injury.

Effect of cisplatin implantation on tumor expression change of SYK, HER2, CD44V6 and PTEN in human gastric carcinoma xenograft of nude mice

Abstract: 【Objective】 To assess the antitumor effects of cisplatin implants vs common cisplatin injection in a xenograft nude mouse model of human gastric cancer and its effect on the tumor expression change of SYK, HER2, CD44V6 and PTEN against human gastric carcinoma xenograft in nude mice, and to discuss its advantage in the treatment of gastric cancer. 【Methods】 A xenograft nude mouse model of human gastric cancer was established by subcutaneously injecting SGC7901 cells in BALB / c nude mice. The model mice were randomly divided into three groups: A(intratumor injection of PBS), B(intratumor injection of cisplatin),and C(cisplatin implantation) with 6 in each group. After drug intervention, tumor weight and volume were calculated. Tumor tissues were observed by HE staining. The protein expressions of spleen tyrosine kinase(SYK), human epidermal growth factor receptor 2(HER 2), phosphate and tension homology deleted on chromosome ten(PTEN), the sixth variant form of adhesion molecule CD44(CD44V6) and cysteinyl aspartate specific protease 3(Caspase 3) / Survivin in tumor tissue were detected by immunohistochemistry. Cell apoptosis was detected by flow cytometry.【Results】 Sustained-release cisplatin significantly promoted apoptosis,suppressed tumor growth, enhanced the expression of SYK and PTEN(that in the group C was higher than those in other groups, P 0.05), reduced the expression of HER 2 and CD44V6(that in the group C was lower than those in other groups, P 0.05). 【Conclusion】 Cisplatin-implants can develop the anticancer function by apoptosis through enhancing the expression of SYK and PTEN, and reducing the expression of HER 2 and CD44V6. It can efficiently kill the tumor, furthermore give new ideas in clinical practice.

Clinical significance of imbalance of CD4+T cells subgroups in COPD

Abstract: 【Objective】 To research changes and significances of CD4 +T cells subgroup in different stages of COPD 【Methods】The number of T cells was counted, and Treg, Th17, Th1, Th2 proportion of CD4+T cells were detected with flow cytometry in different stages of COPD and control group 【Results】The number of T cells and the proportion of Th1 cells, Th2 cells, Th17 cells, Th17/Treg in acute phase of COPD group were higher than those in stable phase of COPD group(P 005); but Treg,Th1/Th2 were lower(P 005); The proportion of Th1 cells, Th17 cells, Treg cells and Th1/Th2, Th17/Treg in stable phase of COPD group were higher than those in control group(P 005), but Th2 cells were lower(P 005), the change of T cells were not statistically significant differences(P 005)【Conclusion】The imbalance of CD4+T cells subgroup plays an important role in the pathogenesis of COPD