Hemoglobin 

About: Hemoglobin is an academic journal published by Marcel Dekker. The journal publishes majorly in the area(s): Thalassemia & Gene. It has an ISSN identifier of 0363-0269. Over the lifetime, 3002 publications have been published receiving 30506 citations. The journal is also known as: Hb & Hgb.

Construction of Human Gene Libraries from Small Amounts cf Peripheral Blood: Analysis of β-Like Globin Genes

Abstract: We describe a rapid procedure for constructing cloned human genomic libraries from small amounts of peripheral blood High molecular weight DNA is isolated from 5–20 ml peripheral blood, partially cleaved with Eco R1, and 8–22 kb fragments are cloned using bacteriophage Charon 4A and a suitable Ecoli host Using this approach we have isolated and characterized several non-a globin clones from a Kurdish Jew with homozygous β thalassemia The ability to isolate suitable amounts of high molecular weight DNA from peripheral blood provides a relatively simple means of constructing human gene libraries representing a variety of hemoglobin disorders

Hemoglobinopathies in Southeast Asia

Abstract: Cet article etudie successivement la frequence de la thalassemie α, de la thalassemie β, des variants genetiques de la chaine α et de la chaine β, de l'hemoglobine constant spring et de l'HbE

Separation of human hemoglobins by DEAE-cellulose chromatography using glycine-KCN-NaC1 developers.

Abstract: This chromatographic procedure uses DEAE-cellulose as ion exchanger and glycine-KCN-NaCl solutions as developers Blood samples from several adults and newborn infants with α, β, δ, or γ chain variants have been analysed The hemoglobins are eluted as compact and symmetrical zones, and the separation of many hemoglobin types is greatly improved The procedure is relatively fast, simple, and inexpensive

Hemoglobinopathies in Southeast Asia: Molecular Biology and Clinical Medicine

Abstract: Thalassemia (thal) and hemoglobinopathies are widespread, recessive inherited diseases. Approximately 250 million people (4.5% of the world population) carry abnormal hemoglobin (Hb) genes. Southeast Asia consists of 10 countries, i.e. Brunei, Cambodia, Indonesia, Laos, Malaysia, Myanmar, the Philippines, Singapore, Thailand, and Vietnam, with a total population of more than 400 million. In most of these countries the population is ethnically heterogeneous. Thalassemias are common in Southeast Asia. α-Thalassemias are very prevalent, attaining frequencies of 20–40%. β-Thal, Hb Constant Spring (CS) and Hb E are also common, the latter attaining a frequency as high as 50–60% among certain populations (1). Abnormal genes in different combinations lead to more than 60 different thalassemic syndromes. The thalassemia disorders were originally confined to the tropics and subtropics. However, Southeast Asians are now scattered around the world and have carried with them most of the complex thalassemic conditions...

The beta-thalassemia mutation spectrum in the Iranian population.

Abstract: β-Thalassemia is the most common hereditary disease in Iran. More than two million carriers of β-thalassemia live in Iran. Since the Iranian population is a mixture of different ethnic groups, it is necessary to determine the frequency and distribution of mutations in the different parts of the country. For this purpose, we divided Iran in to eight different regions according to the geographic and ethnic distribution of the population. Over a 10-year period 1,217 β-thalassemia chromosomes of 164 affected patients and 889 unrelated carriers were studied using the amplification refractory mutation system-polymerase chain reaction technique. We detected 81% β-thalassemia mutations in the studied chromosomes. IVS-II-I (G → A) was the predominant mutation found in our study (34%). Its relative frequency in the north was much higher than other regions, and it lessened toward the south, where the IVS-I-5(G → C) mutation was more common. IVS-I-5 (G → C) (7.55%), codons 8/9 (+G) (4.76%), and IVS-I-110 (G → A) (4.7...