Showing papers in "International Archives of Allergy and Immunology in 1973"
324 citations
294 citations
TL;DR: Bilateral symmetrical electrolytic lesions were produced in the following areas of the rat brain: hypothalamus, reticular formation, thalamus, superior colliculus, caudate nucleus and amygdaloid nucleus.
Abstract: Bilateral symmetrical electrolytic lesions were produced in the following areas of the rat brain: hypothalamus, reticular formation, thalamus, superior colliculus, caudate nucleus and amygdaloid compl
172 citations
TL;DR: The high incidence of lymphocytes showing antibody opsonic adherence in patients with chronic lymphatic leukaemia, together with the finding that most lymphocytes binding sensitized ox red cells did not bind unsensitized sheep red cells (a possible T-cell marker) suggests that lymphocytes representing a subpopulation of peripheral blood lymphocytes might be an analogue of the B-cell population in mice.
Abstract: Ox red blood cells sensitized with rabbit anti-ox red cell antibody adhered opsonically to freshly prepared human peripheral blood lymphocytes. When tested by a centrifugation technique 24–59% of live lymphocytes in normal individuals formed rosettes with the indicator red cells while 41–87% of the lymphocytes in chronic lymphatic leukaemia reacted. After storage at + 4 °C the lymphocytes gradually lost the operative receptor; killing the cells by freezing and thawing, by sodium azide or by fixation in formaldehyde also resulted in loss of capacity to bind the indicator cells. Pretreatment of the lymphocytes with high doses of anti-human IgM in diluted serum resulted in a definite, although incomplete, inhibition. Also, soluble antigen-antibody complexes were inhibitory, although strong inhibition was difficult to show. Undiluted, heated normal human or rabbit serum was highly inhibitory. The high incidence of lymphocytes showing antibody opsonic adherence in patients with chronic lymphatic leukaemia, together with the finding that most lymphocytes binding sensitized ox red cells did not bind unsensitized sheep red cells (a possible T-cell marker) suggests that lymphocytes showing antibody opsonic adherence represent a subpopulation of peripheral blood lymphocytes. This subpopulation might be an analogue of the B-cell population in mice.
171 citations
TL;DR: Vitamin E, supplemented to semisynthetic or natural commercial diets in amounts of 60–180 mg/kg increased the humoral immune response of mice against sheep red blood cell or tetanus toxoid antigens by 40%.
Abstract: Vitamin E, supplemented to semisynthetic or natural commercial diets in amounts of 60–180 mg/kg increased the humoral immune response of mice against sheep red blood cell or tetanus toxoid antigens by
104 citations
TL;DR: A proportion of guinea pig lymphoid cells have an affinity for rabbit erythrocytes which is demonstrable by a rosette-forming technique, and in normal adult guinea pigs, this reaction has been shown to be normal.
Abstract: A proportion of guinea pig lymphoid cells have an affinity for rabbit erythrocytes which is demonstrable by a rosette-forming technique. In normal adult guinea pigs, this reaction has been shown to oc
73 citations
TL;DR: The in vivo behaviour of chromium-labelled platelets was studied in normal, C6 deficient and C3-9 depleted rabbits following the injection of a potentially lethal dose of an endotox.
Abstract: The in vivo behaviour of chromium-labelled platelets was studied in normal, C6 deficient and C3-9 depleted rabbits following the injection of a potentially lethal dose of an endotox
65 citations
TL;DR: In two patients with chronic glomerulonephritis who received renal allografts, transplant failure was associated with binding of transplantation antibodies to the graft and with glomerular and vascula failure.
Abstract: In two patients with chronic glomerulonephritis who received renal allografts, transplant failure was associated with binding of transplantation antibodies to the graft and with glomerular and vascula
64 citations
63 citations
TL;DR: Using such a system a preliminary double-blind study of 54 selected sera from patients with various collagen diseases was undertaken, it was found that 11 sera of patients with a diagnosis of systemic lupus erythematosus (SLE) all had ANA titers of 200 or more and all fixed the C4 component of complement.
Abstract: Antinuclear antibody (ANA) titers in complement immunofluorescence (CIF) and conventional indirect immunofluorescence (IIF) staining were established and CIF/IIF ratios of ANA titers calculated. The following properties of conjugates seem to be indicated for determinations of CIF/IIF ratios: (1) anti-C4 conjugate dilutions used for CIF staining of ANA should be adjusted to contain ½ unit of antibody/ml; (2) anti-IgG conjugates used for this purpose should be adjusted to ¼ unit/ml; (3) for determinations of CIF/IIF ratios of ANA titers, the two conjugates used should have comparable F/P ratios or optical sensitivity ratios. Using such a system a preliminary double-blind study of 54 selected sera from patients with various collagen diseases was undertaken. After completion of titrations and decoding, it was found that 11 sera of patients with a diagnosis of systemic lupus erythematosus (SLE) all had ANA titers of 200 or more and all fixed the C4 component of complement. The CIF/IIF ratio of these 11 sera fell in the range of 1:6 to 1:1. Of the remaining 43 sera 4 fixed complement. Of these, 2 had antinucleolar antibodies rather than true ANA. Of the other 2 sera which fixed C4, one was from a case of discoid lupus erythematosus and another from a patient with rheumatoid arthritis. Their CIF/IIF ratios were 1:8 and 1:21, respectively. Tests for ANA patterns of 25 coded sera in another laboratory yielded peripheral staining with 3 of 4 SLE sera and with none of the other sera included.
57 citations
TL;DR: The ontogeny of the lymphoid cells in conventional and germ-free mice has been studied and development is similar in both groups up to the age of 4 weeks.
Abstract: The ontogeny of the lymphoid cells in conventional and germ-free mice has been studied. Development is similar in both groups up to the age of 4 weeks. During the first 2 weeks of neonatal life, few c
TL;DR: The results clearly show that under the test conditions employed surface immunoglobulins are markers of a bursa cell population or of bursA-derived cells, whereas thymus cells react only minimally with anti-immunoglobulin conjugates in young chickens.
Abstract: The percentage of lymphoid cells carrying immunoglobulin surface determinants was examined for the yolk sac, bursa of Fabricius, thymus, bone marrow, blood lymphocytes, spleen, cecal tonsils and Harder’s glands in normal White Leghorn chickens from the 11th day of incubation until the 26th week after hatching, using direct immunofluorescent staining with a fluoresceinated rabbit anti-chicken immunoglobulin serum. The results clearly show that under the test conditions employed surface immunoglobulins are markers of a bursa cell population or of bursa-derived cells, whereas thymus cells react only minimally with anti-immunoglobulin conjugates in young chickens. The migration pattern and maturation of bursa cells were studied chronologically as reflected in appearance, quantity and morphology of immunoglobulin surface determinant bearing cells in different organs.
TL;DR: Allergens from dog serum and extracts of dog hair, dandruff and skin scrapings were studied with respect to some physico-chemical and immunological properties.
Abstract: Allergens from dog serum and extracts of dog hair, dandruff and skin scrapings were studied with respect to some physico-chemical and immunological properties. Serum albumin was found to be an importa
TL;DR: The influence of two different commerical diets on immune parameters of 8–11-month-old New Zealand Black (NZB) female mice has been presented.
Abstract: The influence of two different commerical diets on immune parameters of 8–11-month-old New Zealand Black (NZB) female mice has been presented. A diet relatively high in fat and low in protein favored
TL;DR: From the sera of 23 thermally injured patients studied, 8 patients showed with the indirect IFT-test on guinea pig epithelium the appearance of circulating pemphigus-like antibodies belonging to diffe.
Abstract: From the sera of 23 thermally injured patients studied, 8 patients showed with the indirect IFT-test on guinea pig epithelium the appearance of circulating pemphigus-like antibodies belonging to diffe
TL;DR: Since immunization of guinea pigs with homologous retina can elicit experimental allergic uveitis concomitant with development of tissue specific antibodies, an attempt was made to localize a uveitoge.
Abstract: Since immunization of guinea pigs with homologous retina can elicit experimental allergic uveitis concomitant with development of tissue specific antibodies, an attempt was made to localize a uveitoge
TL;DR: The K antibody response was studied in immunized rabbits and in children with urinary tract infection, finding a relatively marked and regular antibody response with both β-mercaptoethanol sensitive and resistant antibodies.
Abstract: The K antibody response was studied in immunized rabbits and in children with urinary tract infection. Hyperimmunization of rabbits with K antigen-containing bacteria gave a relatively marked and regular antibody response with both β-mercaptoethanol sensitive and resistant antibodies. Purified K antigen only in a few animals gave very low K antibody titres. Tolerance induction in rabbits was possible using purified K antigen. In patients, K antibodies were very seldom demonstrated. Five occasions of pyelonephritis out of 9 in 8 patients with acute pyelonephritis caused by E. coli O6:K2a,2c or O6:K13 and only 2 patients out of 17 having K1-containing strains gave an antibody response to the K antigen although they all formed antibodies to the·antigen of the infecting bacteria. No K antibodies were found in patients with cystitis.
TL;DR: Articular cartilage from the 3rd and 4th metacarpals of young pigs was maintained for 10 or 14 days in organ culture, in either control media or medium containing rabbit antiserum to pig red cells.
Abstract: 1. Articular cartilage from the 3rd and 4th metacarpals of young pigs was maintained for 10 or 14 days in organ culture, in either control media or medium containing rabbit antiserum to pig red cells
TL;DR: Results show that a third component derived from bone marrow is required for worm expulsion and is additional to the humoral (worm damage by antibody, and cell-mediated components previously defined by the studies of Jones and Ogilvie [1971], Kelly and Dineen [1972a], Keller and Keist [1972] and Dinesen et al.
Abstract: Reconstitution of heavily irradiated rats (750 rad whole-body irradiation) with mesenteric lymph node cells from immune donors and bone marrow cells from either immune or normal (non-immune) donors caused the expulsion of transplanted damaged worms of Nippostrongylus brasiliensis. These results show that a third component derived from bone marrow is required for worm expulsion and is additional to the humoral (worm damage by antibody) and cell-mediated components previously defined by the studies of Jones and Ogilvie [1971], Kelly and Dineen [1972a], Keller and Keist [1972] and Dineen et al. [1973b].
TL;DR: The microtubular disruptive drugs colchicine and vinblastine were found to have a strong enhancing effect on the in vitro migration of normal guinea pig peritoneal exudate macrophag.
Abstract: The microtubular disruptive drugs colchicine and vinblastine were found to have a strong enhancing effect on the in vitro migration of normal guinea pig peritoneal exudate macrophag
TL;DR: Serum albumin was found to be an important allergen in horse epithelia extracts and its concentration varied considerably, depending on the raw material used for the preparation of the allergan.
Abstract: Serum albumin was found to be an important allergen in horse epithelia extracts. The albumin concentration varied considerably, depending on the raw material used for the preparation of the allergen e
TL;DR: The pathogenic retina antigen, previously localized by immunofluorescence to the photoreceptor cell layers, was identified as a soluble protein having an estimated molecular weight of 55,000 and could not be separated into two components by electrophoresis or chromatography.
Abstract: The pathogenic retina antigen, previously localized by immunofluorescence to the photoreceptor cell layers, was identified as a soluble protein having an estimated molecular weight of 55,000. The antigen was separated from guinea pig retina homogenate by successive fractionation techniques using ultra-centrifugation, ammonium sulfate precipitation, and chromatography. Electrophoretic and immunoelectrophoretic analyses indicated that the antigen was obtained in a highly purified form, and as little as 5 μg mixed with adjuvant produced 100% incidence of uveitis in guinea pigs. Formation of a double precipitin band was observed by immunodiffusion and immunoelectrophoresis on testing both crude and purified preparations against homologous guinea pig antiserum. Although this finding suggested antigenic heterogeneity among molecular components of the antigen, the antigen could not be separated into two components by electrophoresis or chromatography.
TL;DR: The occurrence of DNA synthesis in mouse peritoneal macrophages was estimated autoradiographically in cells cultured on glass in the presence of tritiated thymidine and the ability to respond in this way was passively transferable by means of spleen cells, but not serum, from immunized mice.
Abstract: The occurrence of DNA synthesis in mouse peritoneal macrophages was estimated autoradiographically in cells cultured on glass in the presence of tritiated thymidine Subcutaneous injection of a soluble antigen, human serum albumin (HSA) into mice previously immunized with HSA in Freund’s complete adjuvant was followed, after a lag of at least 16 h, by the onset of DNA synthesis in peritoneal macrophages This effect was immunologically specific and was accompanied by morphological changes indicating activation The ability to respond in this way was passively transferable by means of spleen cells, but not serum, from immunized mice DNA synthesis in macrophages was induced in vivo by Concanavalin A, phytohaemagglutinin-P, anti-lymphocyte globulin, bacterial endotoxin and normal mouse serum The number of macrophages synthesizing DNA was also increased in mice with graft-versus-host reactions and in mice bearing a syngeneic methylcholanthrene-induced tumour
TL;DR: Leucocyte migration inhibition tests were carried out in patients with thyroid disease and healthy controls using purified human thyroid microsomes and thyroglobulin as the antigens.
Abstract: Leucocyte migration inhibition tests (LMT) were carried out in 49 patients with thyroid disease and 47 healthy controls using purified human thyroid microsomes and thyroglobulin as the antigens. Patie
Journal Article•
TL;DR: Results in the rat show that the retinal circulation is the source of inflammatory infiltrates which moved progressively from the inner vascular to the outer avascular layers of the retina and destroyed the photoreceptor cells, the retina structure with which the pathogenic antigen has been identified by fluorescent antibody staining.
Abstract: Although uveitis has been widely identified as the main feature of the autoimmune disease produced in several vertebrate species immunized with retina, the present study shows that retinitis is the main feature of the disease in rats. These species differences can be correlated with species differences in intraocular vascularization, and the hematogenous pathways by which the inflammatory cells reach the ocular target. Results in the rat show that the retinal circulation is the source of inflammatory infiltrates which moved progressively from the inner vascular to the outer avascular layers of the retina and destroyed the photoreceptor cells, the retina structure with which the pathogenic antigen has been identified by fluorescent antibody staining.