Showing papers in "Journal of Pharmacological Methods in 1989"

TL;DR: In the present study, a simple linear equation was derived, which in anesthetized dogs corrects more adequately for changes in heart rate than the Bazett formula.

TL;DR: There is a direct linear relation between the concentration of a molecule in the medium surrounding the dialysis membrane and the concentration measured in the collected perfusate, and relative changes of molecular concentration in brain extracellular space may be calculated even when the molecular diffusion rate is unknown.

TL;DR: Tissue exudation and luminal transudation of two plasma markers, Evans blue (EB) dye and [125I]-human serum albumin (HSA), into the airways of the anesthetized guinea pig in response to platelet activating factor (PAF).

TL;DR: The kinetics of intracerebral microdialysis are studied mathematically and it is found that the factor limiting the transport is the diffusion through the brain and not through the membrane.

TL;DR: The present results demonstrate the hyperalgesic effect of naloxone after systemic administration in the tail immersion test and supports the concept that tail flick response is tonically inhibited by endogenous opioid systems.

TL;DR: The telemetry system for the recording of core temperature and activity provides a more accurate assessment of the temporal effects of various drugs and is more efficient and less labor intensive than the use of a rectal temperature probe.

TL;DR: An isocratic high-performance liquid chromatographic (HPLC) method was developed for the simultaneous determination of lactate, pyruvate, and ascorbate in tissue and blood, sampled in vivo using the microdialysis technique.

TL;DR: A novel, eight-chamber superfusion system that is suitable for a variety of applications involving the study of both contraction and relaxation of smooth muscle preparations, and the effect of agents that interfere with these actions is developed.

TL;DR: The data suggest that electromagnetic flowmeters may be used to measure portal venous and hepatic arterial flow simultaneously in anesthetized rats, and offers a clear advantage over other methods of hepatic flow measurement.

TL;DR: This preparation was found to be suitable for the study of NMDA and glycine receptor regulation of the associated ion channel, as these effectors increased the affinity with which [3H]TCP bound to the PCP receptor believed to be located within the ion channel.

TL;DR: The data suggests that CMG parameters and the effects of oxybutynin were not affected by Urethane anesthesia, making the in vivo urethane-anesthetized guinea pig preparation a valuable tool to evaluate both the filling and voiding phases of cystometry.

TL;DR: An epicardial surface ECG recording technique is developed to detect continuously sinus node and His-bundle activities in spontaneously beating Langendorff perfused heart preparations and provided a way to improve or replace more elaborate intracardiac techniques used in isolated hearts or in animal experiments.

TL;DR: The present study shows that specific classes of drug have defined effects on acute-phase protein concentration, and believes that the multiple analysis of APR levels during the course of inflammation may help to distinguish between and elucidate the mechanisms of action, of antiinflammatory and antirheumatic drugs.

TL;DR: Compared to the conventional Boyden chamber technique, this method offers significant advantages, including improvement of sensitivity to chemoattractant so as to detect 0.025% zymosan-activated serum, elimination of tedious filter staining and microscope counting, and permits the use of a microplate reader for instant measurement of PMN migration.

TL;DR: The ability to detect the antifibrillatory activity of quinidine and bretylium suggests that the anesthetized rabbit may provide a useful alternative or additional model for the study of arrhythmias induced by acute myocardial ischemia.

TL;DR: Data show that inotropic responses in NYHA II-III, HAT, WK, and SHR differ from the severely failing myocardium, and it is concluded that new positive inotropic agents should be screened in human myocardial tissue from patients with heart failure.

TL;DR: A rapid and simple method using a combination of high-performance liquid chromatography with electrochemical detection (ECD) and an immobilized enzyme column herein is described for simultaneous assay of acetylcholine (ACh) and choline (Ch) in discrete brain areas of the mouse.

TL;DR: Neither EB nor TTC significantly interfered with the measurement of MPO activity so that EB and/or TTC-stained tissue can be utilized to examine the role of PMN's in myocardial infarction.

TL;DR: Dose-effect relationships have been described for ADP, collagen, and PAF, and effects of drugs upon selected responses illustrate the potential of this test system for routine in vivo screening of agents that may inhibit aggregation of platelets.

TL;DR: The minimal tissue disruption associated with this procedure, as well as its speed, simplicity, and relatively low cost, suggest that the myocardial slice preparation provides an important methodology for the study of beta-adrenergic receptor binding in the semiintact myocardium.

TL;DR: Selective perforation of either one or two cusps of the aortic valve can be performed with ease using touch alone and provides a simple method for producing graded cardiac hypertrophies in rats due to pure volume overload.

TL;DR: A very rapid and simple chromatographic procedure using high-performance liquid chromatography with electrochemical detection in combination with precolumn o-phthalaldehyde derivatization to measure GABA levels in discrete brain regions of mice treated with aminooxyacetic acid and isoniazid is described.

TL;DR: It is concluded that arrhythmia is preceded by a state of deteriorated epicardial activation pattern (prearrhythmmia), which can be quantitatively evaluated and used for detection of preventive antiarrhythmic drug action.

TL;DR: The results suggest that the urethral contractile responses to hypogastric nerve stimulation as well as i.a. phenylephrine were mediated via α 1 -adrenoceptors, whereas the IBP increasing effect of hypogastsic nerve stimulation was not mediated via β-adrenOceptors.

TL;DR: It is concluded that in conscious dogs, reperfusion-induced arrhythmias do not occur after short-lasting myocardial ischemic episodes, and this new experimental approach promises to be of clinical relevance for investigations on the therapeutic efficacy of new antiarrhythmic drugs.

TL;DR: This model provides a well-defined in vivo assay with which to quantify the systemic effects of compounds capable of altering the activity of IL-1, and the data suggest that this mechanism may explain the unique efficacy of steroids as antiinflammatories.

TL;DR: A new and automated on-line analysis of the parameters related to the neuromuscular activity is shown and the procedure used to obtain in real time the statistical evaluation of the recorded electrical signals is presented and discussed.

TL;DR: The use of footprints to measure ataxia, with or without computer aid, is recommended as a routine test in laboratory evaluation of psychoactive drugs and other uses discussed include determining changes in different characteristics of gait in animal and human subjects affected not only by drugs, but also by movement disorders such as Parkinsonism.

TL;DR: It is suggested that the susceptibility of red cells to in vitro oxidative damage may provide a useful functional assessment of generalized alterations in antioxidant status produced by pharmacological interventions.

TL;DR: It could be demonstrated that the long-term assessment of reperfusion arrhythmias by ECG monitoring using the Holter technique is feasible in conscious dogs and represents a promising approach to clinically relevant experimental investigations on the therapeutic efficacy of a new antiarrhythmic drugs.