Showing papers in "Journal of Shenyang Pharmaceutical University in 2008"

Chemical constituents of the aerial parts of Polygonum capitatum

Abstract: Objective To study the chemical constituents of the 70% ethanol extract of the aerial parts of Polygonum capitatum Buch-Ham ex D.Don.Methods The constituents were isolated by several chromatographic methods and identified by physico-chemical characters and spectroscopic analysis.Results Eleven compounds were isolated and identified as:quercetin(1),kaempferol(2),quercitrin(3),protocatechuic acid(4),gallic acid(5),protocatechuic acid ethyl ester(6),ethyl gallate(7),vanillic acid(8),1,5,7-trihydroxyl-3methylanthraquinone(9),succinic acid(10),5-hydroxymethylfuraldehyde (11).Conclusions Compounds 6,7,9 and 11 are isolated from this genus for the first time.

Scopolin isolated from Erycibe obtusifolia Benth stems suppresses adjuvant-induced rat arthritis by inhibiting inflammation and angiogenesis

Abstract: Objective To study the effects and mechanisms of scopolin isolated from the stems of Erycibe obtusifolia Benth in arthritis-associated inflammation and angiogenesis.Methods Adjuvant-induced arthritic rat,an animal model for human RA was used in this study for examining the potential remedial effect of scopolin.The swelling in both inoculated and non-inoculated paws,body weights and articular index(AI)scores were detected to evaluate the severity of the arthritis.Histologic assessment of tissue sections from rat ankles was also performed.Furthermore,the blood vessel density in the synovial tissues was quantitatively evaluated.In addition,expressions of VEGF,FGF-2,TNF-α,IL-1β and IL-6 in rat synovial tissues were determined by immunohistochemistry assay in an attempt to explain the mechanisms of scopolin for suppressing arthritis.Results Scopolin dose-dependently inhibited both inoculated and non-inoculated paw swelling in rat AIA.The mean AI scores of scopolin treated groups were also dose-dependently lower than that of model group.In addition,compared with the weights of model group,the mean body weights of rats treated with scopolin(50,100 mg·kg-1)were higher from day 13 to 22,perhaps indicative of healthier animals.The histologic architecture of the joint was highly abnormal in the model group rats,while high dose of scopolin treated rats preserved a nearly normal histologic architecture of the joint.Moreover,the new blood vessels were reduced dose-dependently in the synovial tissue of rat AIA treated with scopolin.Further,scopolin reduced the overexpression of IL-6,VEGF and FGF-2 in rat synovial tissues.Conclusions Scopolin is capable of reducing clinical symptoms of rat AIA by inhibiting inflammation and angiogenesis,and this compound may be a potent therapeutic agent for angiogenesis related diseases and can serve as structural base for screening for more potent synthetic analogs.

Chemical constituents of Artemisia annua L.

Abstract: Objective To study chemical constituents from the the leaves of Artemisia annua.Methods Compounds were isolated and purified by column chromatography over Sephadax LH-20 and silica gel column.Their chemical structures were elucidated on the basis of physicochemical properties and spectral data.Results Thirteen compounds were isolated,purified,and identified as:artemisinin(1),arteannuin B(2),3α-hydroxy-1-deoxyartemisinin(3),artemisinic acid(4),artemetin(5),chrysosplenetin(6),quercetagetin-3,7,3′,4′-tetramethyl ether(7),chrysosplenol D(5,3′,4′-trihydroxy-3,6,7-trimethoxyflavone,8),salicylic acid(9),domesticoside(2-O-βD-glucopyranosyl-4-O-methyl phloracetophenone,10),scopolin(11),β-sitosterol(12),daucostrol(13).Conclusions Compounds 14 belong to biosynthetic related sesquiterpenoid,and compound 10 is isolated from the Compositae family for the first time.

Phenylpropanoids compounds isolated from Drynaria fortunei and their proliferation effects on UMR 106 cell

Abstract: Objective To study the phenylpropanoid constituents from Drynaria fortunei and the effects of isolated compounds on the proliferation of the rat osteoblastic UMR 106 cells.Methods The constituents in the 60% ethanol extract of Drynaria fortunei were isolated by a combination of silica gel,Sephadex LH-20 and ODS chromatographies and their structures were identified by spectral methods.Their effects on the proliferation activities of UMR 106 cell were measured.Results Seven known phenylpropanoids and two benzoic acid derivatives were isolated from Drynaria fortunei.Their structures were identified by means of spectroscopic analysis as:(E)-caffeic acid 4-O-β-D-glucopyranoside(1),trans-p-(sinapoyl)-β-D-glucopyranoside(2),ferulic acid β-glucoside(3),trans-caffeic acid(4),p-coumaric acid 4-O-β-D-glucopyranoside(5),dihydroisoferulic acid(6),dihydrocaffeic acid(7),vanillic acid 4-O-β-D-glucoside(8),protocatechuic acid(9).Their effects on the proliferation activities of UMR 106 cell were measured.Conclusions Compounds 2-8 are obtained from this genus for the first time,while compound 1 is firstly isolated from this species.The effects of compounds 1-9 are tested on UMR 106 cells,among them,1-4 increase the proliferation activities of UMR 106 cells.

Chemical constituents of the extraction of bamboo leaves from Phyllostachys nigra(Lodd.ex Lindl.) Munro var.henonis(Mitf.) Stepf.ex Rendle

Abstract: Objective To study the chemical constituents of the extraction of bamboo leaves of Phyllostachys nigra(Lodd.ex Lindl.) Munro var.henonis(Mitf.) Stepf.ex Rendle.Methods The compounds were isolated by chromatography on silica gel column,Sephadex LH-20,open ODS column and identified on the basis of physico-chemical constants and spectral analysis.Results Twelve compounds were isolated from EtOAc soluble fraction and n-BuOH soluble fraction of this plant and identified as β-sitosterol(1),tricin(2),daucosterol(3),butanedioic acid(4),5-methyluracil(5),tricin-7-O-β-D-glucopyranoside(6),vitexin(7),tricin-7-O-neohesperidoside(8),orientin(9),isoorientin(10),uracil(11),thymidine(12).Conclusions Compounds 4,5,6,8,11,12 are obtained from this genus for the first time.

Isolation and identification of chemical constituents of Polygonum perfoliatum L.

Abstract: Objective To study the chemical constituents of Polygonum perfoliatum L.Methods The constituents of the ethyl acetate-soluble and n-BuOH-soluble portions of the 70% ethanol extract of Polygonum perfoliatum L.were isolated and purified by various chromatographic methods.The structures were confirmed on the basis of physico-chemical properrties and spectroscopic data.Results Seven compounds were isolated and identified as kaempferol(1),quercetin(2),avicularin(3),quercetin-3-O-β-D-glucuronide 6″-methyl ester(4),quercetin-3-O-β-D-glucuronide 6″-butyl ester(5),kaempferol-3-O-rutinoside(6) and rutin(7).Conclusions Compound 5 is obtained from this genus for the first time;Compounds 3,6 and 7 are isolated from this plant for the first time.

Study on inhibitory effect by total alkaloids in Capparis spinosa on SGC-7901 in vitro

Abstract: Objective To investigate the apoptosis effect of Total alkaloids on human gastric cancer cells SGC-7901 from Capparis spinosa(C.S)and possible mechanism of it.Methods SGC-7901 cells were treated with different concentrations of the Total alkaloids in CS.MTT assay and SRB assay were used to observe the inhibitory rate of the Total alkaloids,and fluorescence microscope,flow cytometry and used to observe the influence of the Total alkaloids on cell apoptosis and cell cycle changes of SGC-7901.Results The results showed total alkaloids can inhibit the growth of human gastric adenoma cells SGC-7901.Measurements using mononuclear cell direct cytotoxicity assay(the MTT method)shows that its cytotoxic effect on SGC-7901 is strong,with IC50 being 142.895 μg·mL-1,respectively.Results from SRB assay show that the anticancer effect of Total alkaloids is cytostatic at low concentration,with LC50 for this cells being 41.271 μg·mL-1,respectively,but it becomes mainly cytotoxic at high concentration,with GI50 for SGC-7901 being 244·932 μg·mL-1,respectively.Total alkaloids can induce apoptosis in tumor cells.Forty-eight hours after they are treated with total alkaloids of different concentrations,SGC-7901 cells are stained with Hoechst33258 fluorochromes.Observation using a fluorescence microscope reveals that total alkaloids can cause the chromatin in tumor cell nuclei to condense and fragment.The nuclei condense into a uniform,dense mass and then break up.Sprouts keep on forming on the cell membrane and then dropping off,so that the cell breaks up into several apoptotic bodies of different sizes.As total alkaloids concentration is increased,these morphological changes under the microscope become more and more clear,indicating that the proportion of cells undergoing apoptosis is gradually increasing.After treating with 75,150 and 300 μg·mL-1 of the total alkaloids in C.S for 72 h,the apoptotic rates of SGC-7901 cells were 8.7%,14.309%,0.819%.Conclusions Inducing apoptosis is one of the anti-cancer mechanism of total alkaloids of C.S.

Antibacterial constituents from the flowers of Trollius chinensis Bunge

Abstract: Objective To study the antibacterial constituents from the water extract of the flowers of Trollius chinensis Bunge.Methods The constituents were isolated by repeated column chromatography on silica gel and Sephadex LH-20 and identified by physico-chemical characteristics and spectroscopic analysis.Results Seven compounds were isolated from the CHCl3 and ethyl acetate extracts,which were identified as:veratic acid(1),benzoic acid(2),(R)-10,16-dihydroxyhexadecanoic acid(3),apigenin(4),luteolin(5),salvigenin(6),and quercetin(7).Conclusions Compounds 24 are isolated from this genus for the first time.

Chemical constituents of thiophenes from Echinops latifolius Tausch

Abstract: Objective To study the chemical constituents of thiophenes from the roots of Echinops latifolius Tausch.Methods The compounds were isolated by chromatography on silica gel column and identified on the basis of physico-chemical properties and spectroscopic analysis.Results Six thiophenes were isolated and their structures were identified as 5-(but-3-en-1-ynyl)-2,2′-bithiophene(1),α-terthienyl(2),cardopatine(3),5-acetyl-2,2′-bithiophene(4),5-(3-acetoxy-4-isovaleroyloxybut-1-ynyl)-2,2′-bithiophene(5) and 5-(4-hydroxybut-1-ynyl)-2,2′-bithiophene(6),respectively.Conclusions Compounds 4-6 are obtained from this plant for the first time.

Chemical constituents from the bulb of Corydalis yanhusuo W.T.Wang

Abstract: Objective To isolate the chemical constituents and elucidate their structures from the bulb of Corydalis yanhusuo W.T.Wang.Methods The compounds were isolated by chromatography on silica gel,Al2O3 column and recrystallization method.NMR,TLC and ESI-MS were employed for the structure identification.Results Thirteen compounds were obtained and elucidated as:tetrahydrocorysamine(1),tetrahydrocoptisine(2),corydaline(3),tetrahydropalmatine(4),dehydrocorydali-ne(5),oxoglaucine(6),noroxyhydrastinine(7),tetrahydroberberine(8),behenic acid(9),β-sitosterol(10),daucosterol(11),vanillic acid(12),p-hydroxybenzoic acid(13),respectively.Conclusions Among these compounds,9,12,and 13 are obtained from the Corydalis genus for the first time;6,7,10 and 11 are obtained from this plant for the first time.

Isolation and identification of chemical constituents from the rhizome of Belamcanda chinensis(L.) DC.

Abstract: Objective To isolate the chemical constituents and elucidate their structures from the rhizome of Belamcanda chinensis(L.) DC.Methods The compounds from the ethanol extraction were isolated by silica gel,polyamide,Sephadex LH-20 and recrystallization,and their structures were identified by the spectral analysis and chemical evidence.Results Nine compounds were obtained and elucidated as:Irisfloretin(1),β-daucosterol(2),dichotomitin(3),irigenin(4),tectorigenin(5),β-sitosterol(6),apocynin(7),uridine(8),and cycloartanol(9).Conclusions Compounds 8 and 9 are isolated from the Belamcanda for the first time.

Determination of sarsasapogenin in Rhizoma Anemarrhenae with precolumn derivatization by HPLC

Abstract: Objective To establish an HPLC-UV method for determination of sarsasapogenin in Rhizoma Anemarrhenae with benzoyl chloride derivate.Methods Chromatography was carried out on Agilent C18 column(150 mm×4.6 mm,5 μm),using methanol-water(V∶V=99∶1) as the mobile phase at a flow rate of 1.1 mL·min-1 and the detection wavelength was set at 230 nm.The column temperature was 33 ℃.Results The linear range of sarsasapogenin was 0.20-4.00 g·L-1(r=0.999 6).The average recoveries of sarsasapogenin was 98.3%(n=9).Conclusions This method can be used for determination of sarsasapogenin in Rhizoma Anemarrhenae.

Chemical constituents from the stems and leaves of marine mangrove plant Pluchea indica(L.)Less.

Abstract: Objective To investigate the chemical constituents of the stems and leaves of Pluchea indica(L.)Less..Methods The compounds were isolated by silica gel chromatography column in associating with Sephadex LH-20 and HPLC chromatography.Their structures were elucidated by spectroscopic analysis.Results Six compounds,including three sesquiterpenes and three thiopene derivatives,were obtained.Their structures were identified as 2-(pant1,3-diynyl)-5-(4-acetoxy-3-hydroxybuta-1-ynyl)-thiophene(1),2-(pant-1,3-diynyl)-5-(4-acetoxy-3-chlorobuta-1-ynyl)-thiophene(2),2-(pant-1,3-diynyl)-5-(3,4-dihydroxybuta-1-ynyl)-thiophene(3),valenc-1(10)-ene-8,11-diol(4),valenc-1(10)-ene-2,8,11-triol(5),1,2,4,8-tetramethyl-(4R,7S,8R,11R)-3-cycloundecenone(6).Conclusions Compounds 26 are isolated from Pluchea indica(L.)Less.for the first time.

Chemical constituents of the whole plant of Scutellaria barbata D.Don

Abstract: Objective To isolate and identify the chemical constituents of Scutellaria barbata D.Don.Methods The compounds were isolated by chromatography on silica gel,prepared thin-layer,Sephadex LH-20 column and recrystallizing method.Physicochemical characters and spectroscopic evidence were employed for the structural identification.Results Seven compounds were isolated and identified as physcion(1),(+)-syringaresinol(2),5,4′-dihydroxy-6,7,3′,5′-tetramethoxyflavone(3),4′-hydroxywogonin(4),6-methoxynaringenin(5),apigenin(6),naringenin(7).Conclusions Compounds 1 and 2 are isolated from Scutellaria for the first time.

Pharmacokinetics and absolute bioavailability of gallic acid in rat plasma

Abstract: Objective To develop a method for the quantitative determination of gallic acid in rat plasma by high performance liquid chromatography(HPLC),and study the pharmacokinetics and absolute bioavailability in ratsMethods Acetaminophen was selected as internal standard,and the plasma samples were pretreated by protein precipitation with methanolPhenomenex C18 was used with the mobile phase of methanol and water contained 05% glacial acetic acid(V∶V=7∶93) at a flow rate of 10 mL·min-1,and detection wavelength was set at UV 272 nmResults After ig of gallic acid,the values of t1/2α,t1/2β,tmax,ρmax and AUC0-t were 4657 min,5654 min,6600 min,396 mg·L-1 and 3965 mg·min·L-1,respectively;As for iv,t1/2α,t1/2β and AUC0-t,the values were 990 min,7888 min and 4619 mg·min·L-1,respectivelyConclusions The concentration-time curves of gallic acid in rats after ig and iv administration shows that they both fit two-compartment modelAbsolute bioavailability of gallic acid is 429%

Chemical constituents of water-soluble part of Mentha spicata L.

Abstract: Objective To study the chemical constituents of water-soluble part of Mentha spicata L.Methods The water-soluble part was treated by methylation.The chemical constituents were isolated by silica gel column chromatography and identified by physicochemical properties and spectroscopic analysis.Results Compounds 15 were obtained and their structures were elucidated as trimethyl citrate(1),methyl protocatechuate(2),methyl vanillate(3),inosine(4),uridine(5).Then the five compounds were demethylated to give the products of citric acid(1′),protocatechuic acid(2′),vanillic acid(3′),inosine(4),uridine(5).Conclusions Compounds 4 and 5 are firstly isolated from the genus Mentha.Compound 1′is isolated from Mentha spicata L.for the first time.

Chemical constituents from the seeds of Castanea mollissima Blume(III)

Abstract: Objective To study the chemical constituents from the seeds of Castanea mollissima Blume and elucidate their structures.Methods The compounds were isolated by column chromatography on silica gel,Al2O3 columns,together with recrystallization methods.NMR,ESI-MS spectral analysis and TLC were employed for the structural identification.Results Six compounds were obtained and elucidated as:p-hydroxybenzoic acid(1),protocatechuic acid(2),gallic acid(3),daucosterol-6′-linoleate(4),ergost-6,22-3β-5α-8α-triol(5),3′,3″-dimethoxylarreatricin(6).Conclusions Among these compounds,compounds 46 were obtained from the Castanea genus for the first time.

Chemical constituents of Castanea mollissima

Abstract: Objective To isolate and identify the compounds from the EtOAc fraction of 90% EtOH extract of seeds of Castanea mollissima Blume.Methods Compounds were isolated by the silica gel and Sephadex LH-20 columns and their structures were identified by the physicochemical characters and spectral analysis.Results Seven compounds were obtained and identified as uracil(1),n-butyl-β-fructopyranoside(2),azelaic acid(3),isoenanthic acid(4),sucrose(5),β-sitosterol(6),daucosterol(7).Conclusions Compounds 1-4 are obtained from Cstanea genus for the first time.

Isolation and identification of chemical constituents from Artemisia capillaries Thunb.

Abstract: Objective To study the chemical constituents of Artemisia capillaries Thunb.Methods Compounds were isolated by the silica gel,Sephadex LH20 columns and the preparative HPLC,and their structure were identified by the physicochemical characters and the spectral analysis.Results Eight compounds were obtained and identified as chlorogenic acid(1),caffeic acid(2),isoquercitrin(3),isorhamnetin-3-O-glucoside(4),6,7-dihydroxyl-coumarin(5),ursolic acid(6),oleanolic acid(7) and β-sitosterol(8).Conclusions Compounds 5,6 and 7 are obtained from Artemisia capillaries Thunb.for the first time.

Chemical constituents of the root of Ampelopsis japonica(Thunb.)Makino

Abstract: Objective To isolate and identify the compounds of the root of Ampelopsis japonica(Thunb.)Makino.Methods The compounds were separated and purified by column chromatography and their structures were established by spectroscopic methods.Results Eight compounds were obtained from the ethyl acetate extracts of the root of A.japonica(Thunb.)Makino.The structures were determined as gallic acid(1),protocatechuic acid(2),gentistic acid(3),reseveratrol(4),emodin(5),stigmasterol(6),β-sitosterol(7),daucosterol(8).Conclusions Compounds 2 and 3 are isolated from the A.japonica(Thunb.)Makino for the first time.

Pharmacokinetics of oleic acid in compound brucea javanica oil submicron emulsions for injection in rats

Abstract: Objective To study the pharmacokinetics of compound brucea javanica oil submicron emulsions,brucea javanica oil emulsions and coix seed oil emulsions in rats.Methods The emulsions were administered through tail vein.A pre-column derivation HPLC-UV method for determination of oleic acid in rat plasma was developed.Results The standard curve was linear over the concentration range of 52.08-868.0mg·L-1(r=0.9992).The method had a high recovery(95%) with intraday and interday precision less than 15%.The pharmacokinetic parameters of oleic acid were evaluated by noncompartmental method.t1/2 were(23.43±6.13)min,(25.56±4.10)min and(21.30±7.34)min,AUC were(61.2±15.2)min·g·L-1,(55.2 ± 12.7)min·g·L-1 and(41.1±15.1)min·g·L-1,respectively.Conclusions The pre-column derivation HPLC-UV method has characteristics of high sensitivity,accuracy and reliability.There are no significant differences amoung the pharmacokinetic parameters of three emulsions.

Membrane separation technique and its application in the field of traditional Chinese medicine

Abstract: Objective To introduce the application and expectation of membrane separation technique in the field of traditional Chinese medicine.Methods The principle,classification,pretreatment,application in the field of traditional Chinese medicine of membrane separation technique were reviewed by consulting the related references.Results The membrane separation technique(microfiltration,ultrafiltration,nanofiltration,reverse osmosis membrane technique) played a extraordinary important role in the field of traditional Chinese medicine.It could be applied in the purification of the extraction of traditional Chinese medicine,the preparation of extracted preparation,the production of oral preparation,the preparation of injection and the removal of heat source and so on.Conclusions The membrane separation technique will play a significant role in the modernization of traditional Chinese medicine and contribute in advancing the normalization standardization of the production of traditional Chinese medicine.

New research progress in pharmacological activities of baicalin

Abstract: Objective To review the new research progress in some pharmacological activities of baicalinMethods Reviews were done based on relative references about baicalinResults Except for traditional effects of baicalin on sedation,hypotension,clearance of free radical and antiarrhythmia,baicalin was proved to possess the therapeutic effects on arteriosclerosis caused by chlamydia pneumoniae,protective effects on ischemia reperfusion injury of brain,heart and hepatic injury induced by different causes,human periodontal ligament fibroblasts;baicalin could significantly inhibit trichomonas vaginalis in vitroConclusions This review provides a reference for the research and development of baicalin

Cathelicidin stimulates colonic mucus synthesis by up-regulating MUC1 and MUC2 expression through a mitogen-activated protein kinase pathway

Abstract: Objective Mucus forms the physical barrier along the gastrointestinal(GI)tract.It plays an important role to prevent mucosal damage and inflammation.Our previous finding showed that antibacterial peptide 'cathelicidin' increased mucus thickness and prevented inflammation in the colon.In the current study,we examined the protective mechanisms by which the peptide increased mucus synthesis in vitro.Methods Human colonic cell line(HT-29)was used to assess the stimulatory action of cathelicidin on mucus synthesis which was measured by the D-[6-3H] glucosamine incorporation assay.Results Human cathelicidin(LL-37)dose-dependently(10-40 μg·mL-1)and significantly stimulated mucus synthesis.Real-time PCR data showed that addition of LL-37 induced more than 50% increase in MUC1 and MUC2 mRNA levels.Treatment with MUC1 and MUC2 siRNAs normalized the stimulatory action of LL-37 on mucus synthesis.LL-37 also activated the phosphorylation of mitogen-activated protein(MAP)kinase in the cells.A specific inhibitor of the MAP kinase pathway,U0126,completely blocked the increase of MUC1 and MUC2 expression as well as mucus synthesis by LL-37.Conclusions Taken together LL-37 stimulates mucus synthesis through the activation of MUC1 and MUC2 expression and the MAP kinase pathway in human colonic cells.

Preparation and characteristics of docetaxel nanos-tructure lipid carriers

Abstract: Objective To prepare docetaxel nanostructured lipid carriers(docetaxel-NLC) and investigate their pharmaceutical properties and drug releasing behavior in vitro.Methods Docetaxel-NLC was formulated by film-dispersion and ultrasonication method.Transmission electron microscopy(TEM) was employed to study the shape.Photon correlation spectroscopy(PCS) was used to study the diameter.The entrapment efficiency was determined by mini-column centrifugation.The in vitro drug releasing behavior was studied by dialysis.Results The mean diameter of NLC was(124±16) nm with Zeta potential of-19.57 mV,and the mean entrapment efficiency was 95.8%.The in vitro drug release was fitted well with Weibull equation.Conclusions Docetaxel-NLC with small particle sizes can improve drug loading capacity and its controlled releasing properties.

Advances in studies on biotransformation of ginsenosides

Abstract: Objective To summarizes the general situation of nature ginsenoside biotransformation in vivo and in vitroMethods Domestic and international references were comprehensively reviewed;a detailed introduction about nature ginsenosides metabolites and pathways were expatiated when in vivo or biotransformed by enzymatic hydrolysis or microorganismResults In human body,nature ginsenosides were transformed into rare ginsenosides which are the really pharmacological componentsMany significant achievements had been reported on ginsenosides biotransformation in vitroConclusions This article will provide references for the metabolism study of ginsenosides and the biological preparation of rare ginsenosides

Determination of the content of the compound clopidogrel bisulfate tablets by HPLC

Abstract: Objective To establish a method for the determination of the content of compound clopidogrel bisulfate tablets.Methods The determination was performed with CN-3 as the chromatographic column.The mobile phase consisted of methanol-water-triethylamine(V∶V∶V=500∶500∶2,adjusted pH to 3.8 by phosphoric acid)with a flow rate of 1 mL·min-1;the detection wave length was 235 nm;the column temperature was 35 ℃,and the sample size was 20 μL.Results Clopidogrel bisulfate could be well separated from aspirin under the conditions mentioned above.The linear relationship between the concentration and area of chromatographic peak were established in the rang of 12-100 mg·L-1 for clopidogrel bisulfate and in the range of 38.4-320.0 mg·L-1 for aspirin.The average recoveries of clopidogrel bisulfate and aspirin were(99.70±1.80)% and(100.30±0.53)%(n=9),respectively.Conclusions The method is easy,exclusive,accurate and suitable for the determination of the content of compound clopidogrel bisulfate tablets.

Isolation and identification of chemical constituents from Cirsium setosum(Willd.) MB.

Abstract: Objective To isolate and identify the chemical constituents from Cirsium setosum(Willd.)MB.Methods The compounds were isolated by chromatography on silica gel column and identified on the basis of physico-chemical constants and spectral analysis.Results Nine compounds were isolated and their structures were identified as tricin(1),linarin(2),caffeic acid(3),courmaric acid(4),succinic acid(5),apigenin(6),tricin-7-O-β-D-glucopyranoside(7),β-sitosterol(8)and β-daucosterol(9),respectively.Conclusions Compounds 5 and 7 are reported from this genus for the first time,compounds 1 and 4 are isolated from this plant firstly.

Megastigmane glucosides from the stems and leaves of the Physalis alkekengi L.var.francheti

Abstract: Objective To study the chemical constituents from stems and leave of Physalis alkekengi L.var.francheti(Mast.)Makino.Methods The leaves and stems of Physalis alkekengi L.var.francheti(Mast.)Makino were extracted with EtOH-H2O(V∶V=60∶40).The extract was partitioned with n-hexane,EtOAc and n-BuOH,respectively.The nBuOH layer was subjected to column chromatography over polyamide,Sephadex LH-20,ODS and RP-PHPLC stepwisely to give four megastigmane glucosides.The spectral analyses and physicochemical characters were used to identify the structures of the compounds.Results All of the four compounds were identified as megastigmane glucosides.They are(6S,9R)-roseoside(1),(6S,9S)-roseoside(2),(6R,9S)-3-oxo-α-ionol-β-D-glucopyranoside(3) and citroside A(4).Conclusions Compounds 14 are isolated from Physalis genus(Solanaceae)for the first time.

Guaiane sesquiterpenes of Curcuma wenyujin

Abstract: Objective To study the chemical constituents of the rhizome of Curcuma wenyujin YHChen et CLingMethods The chemical constituents were isolated by a combination of silica gel,sephadex LH-20,ODS chromatography and HPLCThe structures of these compounds were identified by the physico-chemical evidences and NMR spectroscopic dataResults Seven guaiane sesquiterpenes were isolated from the rhizome of Cwenyujin and identified as zedoarondiol(1),isozedoarondiol(2),aerugidiol(3),zedoalactone A(4),zedoalactone B(5),zedoalactone C(6) and zedoarolide B(7),respectivelyConclusions Compounds 1-7 are obtained from this plant for the first time