Showing papers in "Journal of Steroid Biochemistry in 1970"

TL;DR: It is concluded that the human foetus at midgestation converts cortisol extensively to cortisone, but it is not able to convert the biologically inactive cortis one to the biologically active cortisol.

TL;DR: The enzyme activities of the submicrosomal fraction which were diminished by the gradient centrifugation were largely restored by addition of the heated 105,000 × g supernatant fluid.

TL;DR: A quantitative gas Chromatographic method for the simultaneous determination of 15α-hydroxy-estriol and estriol in pregnancy urine is described and preliminary results obtained with 85 normal urine samples at various stages of pregnancy are presented.

TL;DR: This paper constitutes the first report of the obtainment of an active, cell-free, crude enzyme system from Aspergillus ochraceus, and their radiochemical purity verified by dilution with authentic carrier compounds and crystallization to constant specific activity.

TL;DR: The results demonstrate an extensive parallelism between the plasma concentration and the urinary excretion of oestriol in plasma of pregnant women.

TL;DR: Since under physiological conditions a distinct relationship has been established between sulfoconjugated DHA and human G-6-PDH activity, the endogenous sulfconjugate of DHA may be represented by the lipophile DHA sulfatide rather than by the anionic D HA sulfate.

TL;DR: A mixture of cortisol-3H and cortisone-14C was administered to guinea pig foetusesin situ at mid-term and full-term gestation and to adult guinea pigs and in the liver of the adult animals conversion occurred in both directions.

TL;DR: Various 3β-substituted Δ5-(Δ4) C19- or C21-steroids were tested as inhibitors of human red blood cell glucose-6-phosphate dehydrogenase and it was concluded that the presence of several structural features is required for optimal activity: an oxo group at C-17 or C-20 and a 3-hydroxy group.

TL;DR: The metabolism of six neutral steroids and three steroid conjugates by human prostatic tissue has been investigated and a technique is described for the quantitative determination of radiometabolites from any number of substrates.

TL;DR: Preliminary evidence indicates that in an intact cell system, androstenediol was transformed to compounds with Chromatographie mobilities similar to those of testosterone and dihydro-testosterone, and more firmly bound by nuclei than by cytoplasm.

TL;DR: Gas-liquid chromatography and gas chromatography-mass spectrometry have been used in the identification of six 7-hydroxylated C 19 steroids from human urine, and the Δ 5 -unsaturated steroids were the most abundant of these compounds.

TL;DR: Preliminary evidence is presented for the observation that in urine an equilibrium exists between oestriol-3-glucopyranosiduronic acid and its 3,6 lactone.

TL;DR: β.18-lactone has been isolated from the adrenal venous blood of baboon (Papio hamadryas) and secreted in amounts of about 3 μgl 100 ml adrenal Venous blood.

TL;DR: In this article, the activity of purified glucose-6-phosphate dehydrogenase from human erythrocytes was assayed in the presence of free dehydroepiandrosterone or its different conjugates.

TL;DR: Comparison of the standard curves obtained shows that the slope is optimal with plasma from women pregnant with twins or triplets, and in the last case the curve was almost linear over the range of 0 to 4 ng of testosterone.

TL;DR: The anabolic steroid Oral-Turinabol (4-chloro-17α-methyl-17β-hydroxy-1,4-androstadien-3-one) was hydrogenated with Clostridium paraputrificum and Rhodotorula glutinis as discussed by the authors.

TL;DR: A method for the determination of oestriol in plasma during the second half of pregnancy is described and data on specificity, accuracy, precision, sensitivity, and reproducibility are given.

TL;DR: The neutral steroid disulphate fraction was found to contain 5-androstene-3β,16β,17α-triol, 5-pregnane-3α,20α-diol, 3α,21-dihydroxy-5α-pregnan-20-one, 3β, 21- dihydroxylated steroids, and the main compounds had a 3β-hydroxy-Δ5 structure.

TL;DR: In this article, a relatively simple synthesis of 18-hydroxydeoxycorticosterone via 18hydroxyprogesterone was described, which was used to synthesize 18-HOG.

TL;DR: Both plasma and adrenal tissue from two children with virilizing adrenal carcinoma were analyzed in order to determine their content of unconjugated steroids and of steroid sulfates, finding the individual steroid patterns in the two cases were quite different.

TL;DR: Values suggest that this plasma may be a good source of testosterone binding globulin(s) and that it was higher in late twin pregnancies, with a testosterone binding capacity higher than that for simple pregnancies.

TL;DR: Forty-five male patients suffering from chronic fluorine poisoning and fifteen normal men were investigated in this article, and the urinary excretion of oestrone, oestradiol-17β and oestriol was studied.

TL;DR: In this paper, the effects of various compounds on the seminal vesicle, ventral prostate and levator ani weights in castrated rats were compared for effects upon the three parameters.

TL;DR: Progesterone was found to be transformed by F. oxysporum first to 1:2-dehydroprogestersterone, then to 1;2- dehydrotestosterone, further to 1,4-androstadiene-3,17-dione and finally to 1-deHydrotestololactone as revealed by a thin-layer Chromatographic method.

TL;DR: It appears that the double pH optimum obtained using Oestradiol-3- or 17-phosphates is not linked to a subcellular distribution of acid phosphatase variants with different pH optima.

TL;DR: Of five methods tested for the quantitative recovery of conjugated oestradiol-17β from pregnancy urine, extraction with ethyl acetate at pH 1 to 2 (after making the urine 20% with respect to NaCl) gives the best results.

TL;DR: Plasma levels and uptake by various tissues of 6-methyl-17α-acetoxy-4,6-pregnadiene-3,20-dione and metabolites released from polydimethylsiloxane implants were compared in female and male rats.

TL;DR: Equilibrium dialysis experiments with plasma at several dilutions, and with albumin as reference, demonstrated the presence in plasma of a binding component with a greater affinity for androstenediol than albumin when subjected to equilibrium dialysis.

TL;DR: The quantitative relationship of these 5 oestrogen conjugates changed in the second and third 24-hr urine specimens in which the proportion of the gluco-siduronates of oestrone and 17β-oestradiol fell progressively, while the percentage of the two oestriol glucosiduronates increased.

TL;DR: Cortisol has been isolated from the brain of the rhesus monkey and the amounts per gram of wet tissue averaged 0.55 ± 0.06, in contrast to levels as high as 1–9 μg/g previously reported in human brain.