Medical Journal of Chinese People's Liberation Army 

About: Medical Journal of Chinese People's Liberation Army is an academic journal. The journal publishes majorly in the area(s): Apoptosis & Hepatitis B virus. It has an ISSN identifier of 0577-7402. Over the lifetime, 1239 publications have been published receiving 2330 citations.

Growth characteristics and functional changes in rat chondrocytes cultured in porous tantalum in vitro

Abstract: Objective To evaluate the growth characteristics and functional changes in rat chondrocytes cultured in porous tantalum in vitro. Methods The chondrocytes isolated from cartilage of 3-week old SD rats were cultured in vitro, then the 2nd passage cells were identified and implanted in porous tantalum scaffolds with a density of 1×106 cells/ml. The morphological characteristics of the chondrocytes cultured in porous tantalum were observed under inverted microscope, scanning electron microscope (SEM) and transmission electron microscope (TEM), and the content of glycosaminoglycan (GAG) in the chondrocytes was measured by chromatometry. Results The harvested cells were identified as chondrocytes by type Ⅱ collagen immunocytochemical staining, toluidine blue staining and safranin-O staining. Many chondrocytes adhering to the edge of porous tantalum were found by inverted microscope. Observation under SEM showed that chondrocytes spread well on the surface and distributed in the holes of porous tantalum, and they proliferated and secreted some extracellular matrixes. TEM observation showed that the ultrastructure of chondrocytes cultured in porous tantalum was similar to that of normal chondrocytes. Chromatometry determination showed that the chondrocytes in porous tantalum could secrete GAG continuously. Conclusion Porous tantalum is shown to have a satisfactory biocompatibility with chondrocytes in vitro, and may be used as a scaffold for cartilage tissue engineering. DOI: 10.11855/j.issn.0577-7402.2014.06.08

Re-analysis of predictors of in-hospital mortality in Chinese STEMI patients

Abstract: Clinical predictors of ST-segment elevation myocardial infarction (STEMI) patients may guide clinicians to select the type of treatment. Four articles published in present issue made an in-depth analysis of the data collected recent years from Cardiovascular Intervention Procedures Database of Chinese military hospitals, explored the effects of gender, preoperative severity of target-vessel stenosis, establishment of chest pain center and intra-aortic balloon pump on in-hospital mortality of STEMI patients, which may be helpful for clinicians to individualize treatment, further optimize reperfusion strategy, and improve the clinical efficacy and the prognosis of STEMI patients. DOI: 10.11855/j.issn.0577-7402.2016.06.01

Protective effect of ulinastatin on inhalation lung injury caused by black gunpowder smoke in rats and its inhibitory effect on the mRNA expression of IL-1β and TNF-α

Abstract: Objectives To explore the protective effect of ulinastatin (UTI) on inhalation lung injury caused by black gunpowder smoke in rats and its inhibitory effect on the mRNA expression of interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α). Methods Thirty healthy male Wistar rats were randomly divided into 5 groups (n=6): normal control group (C), inhalation lung injury group (I), high-dose UTI group (UH), medium-dose UTI group (UM) and low-dose UTI group (UL). The rat model of inhalation lung injury was reproduced by a self-made smoke generator. Rats in UH, UM and UL group were given 100 000, 50 000 and 20 000U/(kg.d), respectively, of UTI by intraperitoneal injection. The animals were sacrificed 40h later, then the arterial blood gas values, lung wet-to-dry weight ratio (W/D), white blood cell count and protein concentration in bronchoalveolar lavage fluid (BALF), myeloperoxidase (MPO) activity in serum and lung tissue, and malondialdehyde (MDA) content in lung tissue were determined, and the macroscopic and histological changes in lung tissue were evaluated. The mRNA expression of toll-like receptor 4 (TLR-4), IL-1β and TNF-α were determined by quantitative real-time reverse transcription-PCR (qRT-PCR). Results The values of PaO2 in UH, UM and UL group were higher than that in group I (P 0.05). MPO activity in serum and lung tissue and MDA content in UH group were both lower than those in group I (P 0.05). The mRNA expression of TNF-α in UH group was lower than that in group I, the mRNA expression of IL-1β in UH, UM and UL group was lower than that in group I (P 0.05). The mRNA expression level of TLR-4 in each group was similar (P>0.05). Histopathological observation showed less inflammatory exudates and infiltrations in 3 UTI treated groups than in the I group. Conclusion UTI shows a protective effect on inhalation lung injury caused by black gunpowder smoke in rats through the mechanism of anti-inflammation and anti-oxidation, and high-dose UTI can effectively inhibit the mRNA expression of IL-1β and TNF-α. DOI: 10.11855/j.issn.0577-7402.2014.03.14

Mechanism of the preventive effect of breviscapus on pathogenesis of acute kidney injury in rats with sepsis

Abstract: Objective To investigate the inhibitory effect and its related mechanism of Erigeron breviscapus on pathogenesis of acute kidney injury (AKI) in rats with sepsis. Methods Thirty-six male Wistar rats were randomly assigned into control group, sepsis model group (CLP group), and breviscapine treatment group (treatment group, n=12 each). The sepsis model was reproduced by cecal ligation and puncture (CLP). The rats were sacrificed 24 h after operation. The levels of blood urea nitrogen (BUN) and serum creatinine (Scr) were assayed with enzymatic assays, endothelin 1 (ET-1) and inducible nitric oxide synthase (iNOS) with ELISA, renal malondialdehyde (MDA) and superoxide dismutase (SOD) activities with xanthine oxide method, and the degree of renal tissue injury by micros copic examination after HE staining. Results Compared with the control group, the levels of Scr, BUN, ET-1, iNOS, MDA and iNOS in CLP groups significantly increased, while the SOD activities significantly decreased (P<0.05). Compared with the CLP group, the levels of Scr, BUN, ET-1, iNOS, MDA and iNOS were lower markedly in treatment group, while the SOD activity elevated markedly (P<0.05). Conclusions Breviscapine may reduce AKI in rats with sepsis, and the effect may be related to the improvement of renal microcirculation, reduction of antioxidant enzyme activity, and decrease in oxidation products. DOI: 10.11855/j.issn.0577-7402.2014.11.01

Effect of urinary NGAL on the timing of renal replacement therapy in patients with acute renal injury associated with sepsis

Abstract: Objective To investigate the effect of urinary neutrophil gelatinase-associated lipocalin (uNGAL) on the timing of continuous renal replacement therapy (CRRT) in patients with acute renal injury (AKI) associated with sepsis. Methods One hundred patients with sepsis admitted to Shanxi Grand Hospital from January 2013 to June 2017 were selected as research objects. Sixty patients with uNGAL greater than 1310 ng/ml were defined as high uNGAL group, and 40 patients with uNGAL smaller than 1310 ng/ml were defined as low uNGAL group. The high uNGAL group was randomly assigned to the early CRRT group or standard treatment group. The clinical outcomes were compared between the high uNGAL group and the low uNGAL group. Changes in the levels of uNGAL, TNF-α and IL-6 inflammatory factors at different time points before and after treatment in the early CRRT group and the standard treatment group. Results The 28 d mortality and dialysis dependence rate in the high uNGAL group were significantly higher than those in the low uNGAL group (P<0.05). The patients in the high uNGAL group had less time without mechanical ventilation and less time out of ICU than those in the low uNGAL group (P<0.05). There was no statistically significant difference in mortality between the early CRRT group and the standard treatment group at 28 d. Days without mechanical ventilation and days without ICU stay in the early treatment group were significantly longer than those in the standard treatment group (P<0.05). The ratio of dialysis dependence was lower in the early CRRT group than in the standard treatment group, and the recovery rate of renal function was higher (P<0.05). In the early CRRT group, uNGAL showed a decreasing trend after treatment, which significantly decreased at 48 h (P<0.05). There was no significant difference between the uNGAL levels before and after treatment in the standard treatment group. The levels of IL-6 and TNF-α in the early CRRT group decreased 24 h after treatment, and further decreased 48 h (P<0.05). The levels of IL-6 and TNF-α showed no significant changes after the treatment in standard treatment group, but there were statistically significant changes in the uNGAL and IL-6 levels 48 h after the treatment in the two groups. Conclusion It is feasible to use uNGAL to classify patients with sepsis AKI and uNGALcan be a reliable indicator to guide the initiation of CRRT. DOI: 10.11855/j.issn.0577-7402.2019.07.12