Figure 3. DMAMQ reduces the non-proliferative population of NSCs and increases NSC growth. (a) Flow cytometry analysis of Ki67 proliferation marker expression in NSCs after 24 hours treatment with 2.5 µM DMAMQ. Both populations were gated identically using the unstained background control populations shown in grey behind the Ki67 negative (blue) and Ki67 positive (red) populations. (b) Quantification of the percentage increase in Ki67 expression from the profiles in a; Students t-test, p = 0.025, n = 4. (c) Quantification of βgalactosidase staining following three days incubation with 2.5 μM DMAMQ, change is expressed relative to control cells; Students t-test, n = 4. (d) Flow cytometry profiles of
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