Figure 4. Effects of DMAMQ on NSC differentiation. (a) Immunofluorescence staining demonstrates that NSCs treated with DMAMQ throughout 7 days differentiation retain their capacity to differentiate and are multipotent. Green, NF-L (neuronal marker); Red, GFAP (astrocytic marker); Blue, DAPI (nuclear stain); Scale bar = 20 μm. (b) Example western blot images of cells either differentiated after 24 hours priming with DMAMQ or differentiated in the presence of DMAMQ. Densitometric quantification of western blot intensity for (c) nestin, (d) NF-L and (e) GFAP relative to the total-protein (coomassie) loading control. No differences are observed in lineage preference as a result of DMAMQ exposure either before or during differentiation; one-way ANOVA n = 5.
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