Journal ArticleDOI
A quantitative cytochemical study of the DNA content of neurons of rat cerebellar cortex.
R. D. Lentz,L. W. Lapham +1 more
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Measurements of nuclear DNA content with quantitative cytochemical methods for determining amounts in single nuclei reveal tetraploid quantities of DNA in cerebellar Purkinje neurons of the rat, or twice the amount of rat somatic cells in general.Abstract:
— Measurements of nuclear DNA content with quantitative cytochemical methods for determining amounts in single nuclei reveal tetraploid quantities of DNA in cerebellar Purkinje neurons of the rat, or twice the amount of nuclear DNA of rat somatic cells in general. The findings suggest that tetraploidy is probably a universal phenomenon among rat Purkinje cells. Granule and basket cells have a diploid DNA content.read more
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Neurogenesis in the 3-month-old rat visual cortex.
TL;DR: It has now been confirmed that relatively small labeled neurons and their synapses are found in at least 3 brain regions (olfactory bulb, dentate gyrus, and visual cortex) in a normal adult rodent.
Journal ArticleDOI
Cell cycle activation and aneuploid neurons in Alzheimer's disease.
TL;DR: This finding adds aneuploidy to the list of critical molecular events that are shared between neurodegeneration and oncogenesis and directs attention to a failure of neuronal differentiation control as a critical pathogenetic event and potential therapeutic target in AD.
Journal ArticleDOI
Formation and Turnover of Neurons in Young and Senescent Animals: An Electronmicroscopic and Morphometric Analysis
TL;DR: To determine accurately the types of cells that are formed, the advantages gained by use of r3Hthymidine autoradiography with light microscopy need to be combined with the resolution of electron microscopy.
Journal ArticleDOI
Neuronal polyploidy and nuclear volumes in the cat central nervous system.
TL;DR: Nuclear DNA content has been measured cytophotometrically in neurons and glia of motor cortex, cerebellum, and spinal cord ventral horn of the cat, finding that tetraploid neurons are the efferent neurons of their respective areas, and their nuclei are significantly larger than diploid nuclei.
Journal ArticleDOI
Dna content of purified preparations of mouse purkinje neurons isolated by a velocity sedimentation technique
TL;DR: No evidence could be found for the existence of a tetraploid DNA complement in mouse Purkinje neurons despite indications to the contrary from a parallel cytophotometric study.
References
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Journal ArticleDOI
Autoradiographic investigation of cell proliferation in the brains of rats and cats
TL;DR: The results indicate that glia cells can multiply in the brains of young adult rats and adult cats and they support the possibility that new neurons may be formed in forebrain structures, both in rodents and carnivores.
Journal ArticleDOI
The Desoxyribose Nucleic Acid Content of Animal Nuclei
TL;DR: Evidence has recently been presented that DNA is considerably less variable in amount, and it is suggested that the amount of DNA per nucleus is probably constant in all the nuclei of any one species.
Journal ArticleDOI
An integrating microdensitometer for biological cells
TL;DR: In this article, a photoelectric method for the rapid determination of the total absorption of biological cells is described, where the image of the cell, formed by a microscope, is scanned by a mechanically-driven aperture behind which is mounted a photomultiplier.
Journal ArticleDOI
Quantitative cytochemical determination of desoxyribonucleic acid with the feulgen nucleal reaction
Hans Ris,Alfred E. Mirsky +1 more
TL;DR: It appears possible to determine cytochemically relative amounts of DNA in cellular structures by measuring their absorption after treatment with the Feulgen nucleal reaction.
Journal ArticleDOI
Tetraploid DNA content of Purkinje neurons of human cerebellar cortex.
TL;DR: Microspectrophotometric analysis of single cells in Feulgen preparations revealed tetraploid amounts of DNA in Purkinje neurons of human cerebellar cortex.