Antibodies to Human Seminal Plasma Inhibin Adversely Affect Sperm Function Parameters
M K Mehta,A R Sheth +1 more
TLDR
Sperm plasma membrane was damaged in antibodies treated spermatozoa as evidenced by hypoosmotic swelling test and sperm lipid peroxidation reaction.Abstract:
Polyclonal antibodies to intact inhibin (94 amino acids, R-94, 10.5 kDa) and its sequence specific synthetic fragments (R-9, R-17) were evaluated for their effect on various physical and biochemical parameters of sperm function. Intact inhibit had maximum deleterious effect on quantitative motility and mean forward progression of spermatozoa. Antibodies had no effect on sperm fructolysis and sperm nuclear chromatin decondensation reaction. Sperm plasma membrane was damaged in antibodies treated spermatozoa as evidenced by hypoosmotic swelling test and sperm lipid peroxidation reaction.read more
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Journal ArticleDOI
Development of an assay to assess the functional integrity of the human sperm membrane and its relationship to other semen characteristics
TL;DR: The hypoosmotic swelling technique to evaluate the functional integrity of the sperm membrane appears to give high repeatability and accuracy and is closely correlated to the in-vitro fertilizing ability of spermatozoa.
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The biochemistry of semen and of the male reproductive tract.
TL;DR: Bromural, Its Distribution and Decomposition In the Animal Organism, and its Differential Diagnosis from Neurological Disorders, Acta Neurol.
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Sperm-specific isoantibodies and autoantibodies inhibit the binding of human sperm to the human zona pellucida
TL;DR: In this article, an inverse relationship was found between the number of sperm adherent to the zona and the proportion of sperm exhibiting binding of IgA or IgG immunoglobulins over the head, indicating that the presence of these antibodies in the acrosomal region of the sperm head inhibited their ability to attach to the human zona pellucida.
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Ability of antibody-bound human sperm to penetrate zona-free hamster ova in vitro * †
TL;DR: Results indicate that the sperm-specific antibodies studied in these experiments, although bound to the sperm head, do not cause infertility by interfering with the acrosome reaction or gamete membrane fusion.
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Lipid Peroxidation in Spermatozoa
TL;DR: Cold shock and homogenization release from spermatozoa a substance, presumably a lipid, which provides the substrate for peroxidation, which is probably linked with the decline in motility and respiratory activity, and the structural damage to sperm membranes, which characterize ageing or degenerating spermutozoa.