Cation transport in Serratia marcescens and Serratia marinorubra.
Reads0
Chats0
TLDR
The sodium, potassium, and magnesium ion contents of Serratia marcescens and those of its salt-tolerant relative, S. marinoruba, were determined by atomic-absorption spectrometry and the intracellular K(+) and Mg(2+) contents of both microorganisms were found to be dependent on the ionic strength of the growth or suspending medium.Abstract:
The sodium, potassium, and magnesium ion contents of Serratia marcescens and those of its salt-tolerant relative, S. marinoruba, were determined by atomic-absorption spectrometry. The intracellular K(+) and Mg(2+) contents of both microorganisms were found to be dependent on the ionic strength of the growth or suspending medium. The Mg(2+) content of S. marinoruba was generally greater than that of S. marcescens. The Na(+) content of the cells was normally low and did not increase as the cells aged or when the cells were grown in media of high ionic strength. The transport of K(+) by resting cells suspended in hypertonic solution was studied by chemical and light-scattering techniques and was found to be more rapid in S. marcescens than in S. marinorubra. The slower rate of K(+) transport in S. marinorubra is probably related to the lower glycogen reserves found in resting cells of this microorganism. K(+) transport was found to have a pH optimum of 5.5 to 6.1 for S. marcescens, and the K(m) for K(+) was approximately 1.6 mm. Na(+) and Mg(2+) were not taken up by the cells, although the presence of Mg(2+) tended to decrease rates of K(+) uptake. Tris-(hydroxymethyl)aminomethane, routinely used for resuspending the cells, was apparently taken up by the cells at pH >7.read more
Citations
More filters
Journal ArticleDOI
The architecture of 5S rRNA and its relation to function
George E. Fox,Carl R. Woese +1 more
TL;DR: An extensive comparative analysis of the available primary sequence data on 5S rRNA has been made and a general principle emerges: the natural rotational motion inherent in coiling and uncoiling of nucleic acid helices can be converted quite simply to linear mechanical motion.
Journal ArticleDOI
Effects of growth conditions on the lipid composition of Bifidobacterium bifidum subsp. pennsylvanicum.
TL;DR: Differences in the decline of unsaturated fatty acids and in the degree of replacement of these acids by C12- and C14-fatty acids may be related to the variations in growth in cultures with various Tweens by way of changes in the physical state of the membrane lipids.
References
More filters
Journal ArticleDOI
Cation transport in Escherichia coli. I. Intracellular Na and K concentrations and net cation movement.
Stanley G. Schultz,A. K. Solomon +1 more
TL;DR: Methods have been developed to study the intracellular Na and K concentrations in E. coli, strain K-12, and have been shown to be functions of the extracellular cation concentrations and the age of the bacterial culture.
Journal ArticleDOI
Cation Transport in Escherichia coli: V. Regulation of cation content.
TL;DR: Measurement of cellular K and Na concentrations in growing Escherichia coli indicates that the osmololity of the medium is a major determinant of the cell K concentration, and a mechanism appears to involve an exchange of K for cellular H.
Journal ArticleDOI
On the physical state of the intracellularly accumulated substrates of β-galactoside-permease in Escherichia coli
TL;DR: It is concluded that the bulk of the intracellular galactoside accumulated by the action of galactOSide-permease is a free solute, and if any intrACEllular binding of Galactosides occurs, it is quantitatively insignificant.
Journal ArticleDOI
Aspects of bacterial response to the ionic environment.
TL;DR: The present study focuses on the role of Ions on Membrane Structure and Protein Conformation, and the effects of Osmotic Pressure and Water Activity in this context.
Related Papers (5)
The Interrelationship between Potassium, Magnesium and Phosphorus in Potassium-limited Chemostat Cultures of Aerobacter Aerogenes
Na+ and K+ Gradients and α-Aminoisobutyric Acid Transport in a Marine Pseudomonad
John Thompson,Robert A. MacLeod +1 more