Showing papers in "Biochimica et Biophysica Acta in 1958"

TL;DR: The equations derived here have been applied to various permeability measurements found in the literature, such as the penetration of heavy water into animal cells, permeability of blood vessels, threshold concentration of plasmolysis and relaxation experiments with artificial membranes.

TL;DR: De considerer la lipase pancreatique comme une esterase particuliere destinee a agir sur des esters insolubles au niveau of l'interface separant ces esters de l'eau, un calcul fait intervenir the “concentration molaire interfaciale” du substrat.

TL;DR: Samples of human haemoglobin from normal adults and from sickle-cell anaemic patients have been heat denatured and then digested with trypsin and “fingerprints” show that the two proteins differ in only one peptide.

TL;DR: The most important physicochemical constants of human globin and its coupling capacity for hematin are reported.

TL;DR: The chitInase system of a strain of Streptomyces griseus was resolved into two chitinase with similar enzymic activities and a chitobiase, which is a heat-labile enzyme which can be irreversibly adsorbed on bauxite.

TL;DR: Lysis of E. coli B, Azotobacter vinelandii, Ps.

TL;DR: These fully succinylated protein derivatives, near neutral pH, exhibit large increases in intrinsic viscosity and decreases in sedimentation constant, which indicate an unfolding or expansion of the protein molecules.

TL;DR: N-acetylseryltyrosine was isolated from the chyromotryptic and peptic digests of TMV-protein and the structure of the acetylpeptide purified by ion-exchange chromatography, was studied by the FDNB method, hydrazinolysis and the use of carboxypeptidase.

TL;DR: The identity of lipoyl dehydrogenase and diaphorase has been demonstrated by the constant ratio of the two activities during purification, by inhibition studies and by the direct demonstration that reduced diaphOrase is rapidly reoxidized by lipoic acid and derivatives.

TL;DR: The heavy fraction, which resembles intact mitochondria much more closely than the light, catalyzes the oxidation of citric acid cycle substrates very efficiently with P/O ratios quite close to the assumed theoretical values.

TL;DR: The purification and properties of α-glucosidase from Saccharomyces italicus Y1225 have been studied and it is suggested that substrate hydrolysis involves an ionizable group of pKa 6.6–6.8.

TL;DR: DPNH can replace TPNH although the rate of fatty acid synthesis is much less and the TPNh-dependent α-β unsaturated acyl CoA reductase—a key enzyme in the elongation of the fatty acid chain by the reversal of β-oxidation—cannot be detected in the purified system.

TL;DR: The phase systems have been used for the isolation of the cell walls from Chlorella and Aerobacter and the behaviour of some model particles in these phase systems has been investigated.

TL;DR: The presence of two free hydroxyl groups in the phospholipid was demonstrated by its conversion to an isopropylidene derivative and by oxidation by lead tetraacetate.

TL;DR: It has been found that the pattern of substrate specificity of baker's yeast Hexokinase is broadly similar to that of brain hexokinase, although there are several significant differences.

TL;DR: The application of photometric techniques to a biological problem, the study of permeability, has been discussed and it is pointed out that a number of changes can occur in mitochondria producing changes in optical density which might be spuriously interpreted as volume changes.

TL;DR: The appearance of the highly specific chromatographic pattern of IPYA decomposition provided definite proof for the occurrence of IPyA in the media, and Indolelactic acid and tryptophol were identified as products of physiological origin.

TL;DR: It is concluded that the bulk of the intracellular galactoside accumulated by the action of galactOSide-permease is a free solute, and if any intrACEllular binding of Galactosides occurs, it is quantitatively insignificant.

TL;DR: The results indicate that the algae investigated may be divided into three groups on a basis of their cell wall structure, and Porphyra is quite unique and forms a third group in which mannose replaces as the basic structural unit of the microfibrils.

TL;DR: Fibers of polyinosinic acid produce an oriented X-ray diffraction pattern with a strong meridional spacing, and two layer lines at 9.8 and 5.2 A, which is compatible with a molecule consisting of three helically wound polynucleotide chains which are hydrogen bonded to each other through the purine bases.

TL;DR: The dissociated products from the Mg-deficient particles are nucleoproteins which have the same RNA/protein ratios as the original particle, indicating that Mg is critically involved in the linkage of the nucleoprotein units and is not involved in binding of RNA to protein.

TL;DR: Treatment of mitochondria with deoxycholate solutions of varying strengths resulted in a gradual and reproducible disintegration of the mitochondria and produced a characteristic release of Mg ++ and of material absorbing maximally at 260 mμ from the mitochondia.

TL;DR: When the heavy fraction of beef heart mitochondrial suspensions is exposed to sonic oscillation, a submitochondrial particle can be isolated which is indistinguishable from previously described ETP except that it is capable of coupling the oxidation of succinate and DPNH to phosphorylation.