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Journal ArticleDOI

Clinical Chemistry: Principles and Technics

Rex B. Conn
- 28 Sep 1964 - 
- Vol. 189, Iss: 13, pp 1035-1035
TLDR
Dr. Henry, as director of a large laboratory specializing in clinical chemistry, is in a unique position for acquiring a personal familiarity with a wide variety of chemical methods, and his book covers basic principles of analytical methods such as colorimetry, spectrophotometry, and flame photometry.
Abstract
Dr. Henry, as director of a large laboratory specializing in clinical chemistry, is in a unique position for acquiring a personal familiarity with a wide variety of chemical methods. Surveying the literature of clinical chemistry, evaluating hundreds of published methods, and selecting that small fraction of material which can be included in a book certainly constitute an impressive task. Not since publication of Peters and Van Slyke's classic Quantitative Clinical Chemistry has anyone been courageous enough to undertake this project, and Dr. Henry has succeeded remarkably well. The first chapters of the book cover basic principles of analytical methods such as colorimetry, spectrophotometry, and flame photometry. The remaining chapters deal with analytical methods for specific substances, and for each substance one or two methods are given in sufficient detail to permit use of the book as a laboratory manual. No attempt is made to include methods such as gas chromatography

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Citations
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Journal ArticleDOI

Enzymatic determination of total serum cholesterol.

TL;DR: An enzymatic method is described for determination of total serum cholesterol by use of a single aqueous reagent and has excellent precision.
Journal ArticleDOI

Quantitative Determination of Serum Triglycerides by the Use of Enzymes

TL;DR: A novel method for determining serum triglycerides, in which an enzymatic hydrolysis replaces the more commonly used saponification procedure, which is simple, rapid, and requires only 50 µl or less of sample.
Journal ArticleDOI

Determination of blood glucose using an oxidase-peroxidase system with a non-carcinogenic chromogen

TL;DR: Manual and automated methods for the determination of blood glucose have been devised using an oxidase/peroxidase system, with dl adrenaline, a non-carcinogen, as oxygen acceptor.
Journal ArticleDOI

Measurement of Plasma Glucose, Free Fatty Acid, Lactate, and Insulin for 24 h in Patients With NIDDM

TL;DR: There are differences in substrate level between individuals with normal glucose tolerance and patients with NIDDM and differing degrees of glucose intolerance, unrelated to ambient insulin level, and these changes persist over 24 h.
Journal ArticleDOI

A candidate reference method for determination of total protein in serum. I. Development and validation.

TL;DR: A candidate Reference Method for measuring total serum protein by use of the biuret reaction is developed, and the presence of dextran in serum causes easily detected turbidity, but this interference can be eliminated by centrifuging the reaction mixture.
References
More filters
Journal ArticleDOI

Enzymatic determination of total serum cholesterol.

TL;DR: An enzymatic method is described for determination of total serum cholesterol by use of a single aqueous reagent and has excellent precision.
Journal ArticleDOI

Quantitative Determination of Serum Triglycerides by the Use of Enzymes

TL;DR: A novel method for determining serum triglycerides, in which an enzymatic hydrolysis replaces the more commonly used saponification procedure, which is simple, rapid, and requires only 50 µl or less of sample.
Journal ArticleDOI

Determination of blood glucose using an oxidase-peroxidase system with a non-carcinogenic chromogen

TL;DR: Manual and automated methods for the determination of blood glucose have been devised using an oxidase/peroxidase system, with dl adrenaline, a non-carcinogen, as oxygen acceptor.
Journal ArticleDOI

Measurement of Plasma Glucose, Free Fatty Acid, Lactate, and Insulin for 24 h in Patients With NIDDM

TL;DR: There are differences in substrate level between individuals with normal glucose tolerance and patients with NIDDM and differing degrees of glucose intolerance, unrelated to ambient insulin level, and these changes persist over 24 h.
Journal ArticleDOI

A candidate reference method for determination of total protein in serum. I. Development and validation.

TL;DR: A candidate Reference Method for measuring total serum protein by use of the biuret reaction is developed, and the presence of dextran in serum causes easily detected turbidity, but this interference can be eliminated by centrifuging the reaction mixture.