Journal ArticleDOI
Composition of J.S.B. stain and factors affecting its quality.
H. L. Bami,C. P. Nair +1 more
TLDR
Storage under severe tropical conditions and periodical checks by staining Plasmodium cynomolgi smears revealed that staining solutions oxidized 6-7 hr with a final pH of 7.8 gave optimum results, while cooler storage conditions were most favorable.Abstract:
Several samples of J.S.B. stain (Jaswant Singh and Bhattacharjee, 1944) solution 1 (polychrome methylene blue) were prepared with 3-8 hr for dichromate-acid oxidation and addition of varying quantities of Na2HPO4 buffer for pH adjustment. Storage under severe tropical conditions and periodical checks by staining Plasmodium cynomolgi smears revealed that staining solutions oxidized 6-7 hr with a final pH of 7.8 gave optimum results. Some precipitation of azures, due to heat after 5 mo, adversely affected the quality of staining solutions, while cooler storage conditions were most favorable. Spectrophotometric and chromatographic studies indicated that the J.S.B. solution 1 was composed of blue and purple components, corresponding to higher methylene azures with methylene blue and thionin with allied products respectively.read more
Citations
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Journal ArticleDOI
Romanowsky-type stains in haemotology
TL;DR: This paper presents a systematic literature review of Romanowsky-type stains and the Romanowsky effect and describes the compositions, properties, and methodology of these stains and their applications.
Journal ArticleDOI
Thin-layer chromatographic separation of methylene blue and related thiazine dyes
TL;DR: In this article, a triple development with 95% ethanol-chloroform-acetic acid (85:10:5) on commercial Silica Gel G thin-layers is described.
Journal ArticleDOI
The composition of stains produced by the oxidation of Methylene Blue
TL;DR: In this article, the composition of some stains produced by the oxidation of Methylene Blue has been studied by thin-layer chromatography and various named methods for the production of polychrome mixtures of varying proporitions of up to eleven dyes.
Journal ArticleDOI
Cien años del colorante de Giemsa
TL;DR: Research work associated with the development of Giemsa stain is revived, with emphasis on the methylene blue polychromes.
References
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Journal Article
Rapid Staining of Malarial Parasites by a Water Soluble Stain.
Jaswant Singh,L M Bhattacharji +1 more
TL;DR: During the past year, work has been in progress in the laboratories of the Malaria Institute of India with the object of producing a stain in which readily available ingredients can be substituted for the costlier Romanowsky stains.
Journal ArticleDOI
Histological, chromatographic and spectrophotometric studies of toluidine blue.
J. Ball,D. S. Jackson +1 more
TL;DR: Fractionation of different commercial batches of toluidine blue yielded identical, homogeneous metachromatic dyes, which had a peak absorption at 615 mμ in contrast to that of purified azure A whose peak absorption was at 622.5 mμ.
Journal ArticleDOI
The Oxidation Products of Methylene Blue
W. C. Holmes,B. W. French +1 more
TL;DR: The mechanism of the oxidation of methylene blue varies with the conditions The formation of trimethyl thionin (azure B) and of asymmetrical dimethyl triethyltionolin(azure A) is followed under alkaline conditions by that of dimethyl methyl violet (methylene violet) and under acid conditions by monomethyl thionins (named by authors azure C) as mentioned in this paper.
Journal ArticleDOI
Spectrophotometric Characteristics and Assay of Biological Stains
TL;DR: In this article, the chemical and spectrophotometric methods adopted in the Stain Commission laboratories for the certification of stains are described and the general approach used for re-study of the stains by these methods and the criteria necessary for adoption of the spectrophotonometric method of assay are described.
Journal ArticleDOI
A Giemsa Stain of Quite Constant Composition and Performance, made in the Laboratory from Eosin and Méthylène Blue.
TL;DR: In this paper, the eosinate formula for compounding Giemsa stain from dyes of American manufacture was worked out, and it was hoped that this would solve the problem of duplicating stains satisfactorily.