Journal ArticleDOI
Determination of substances at low concentrations in complex mixtures by isotachophoresis with column coupling
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TLDR
In this article, a system that makes use of two PTFE narrow-bore tubes with different internal diameters is discussed, where the pre-separation narrowbore tube, which has the larger internal diameter, allows work at a higher current density during final separation and detection by means of conductivity and UV absorption detectors.About:
This article is published in Journal of Chromatography A.The article was published on 1979-02-01. It has received 167 citations till now. The article focuses on the topics: Thermal conductivity detector & Tube (fluid conveyance).read more
Citations
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Journal ArticleDOI
Optimization in sample stacking for high-performance capillary electrophoresis
Dean S. Burgi,Ring Ling. Chien +1 more
Journal ArticleDOI
Sample stacking of an extremely large injection volume in high-performance capillary electrophoresis
Ring Ling. Chien,Dean S. Burgi +1 more
TL;DR: Sample stacking is a sample on-column concentration technique in high performance capillary electrophoresis (HPCE) as mentioned in this paper, but the amount of sample which can be loaded into the column in conventional sample stacking is rather limited because of disturbance caused by the low-concentration sample buffer.
Journal ArticleDOI
Field amplified sample injection in high-performance capillary electrophoresis
Ring-Ling Chien,Dean S. Burgi +1 more
TL;DR: In this article, a simple on-column concentration technique in high-performance capillary electrophoresis (HPCE) is reported, where a short plug of water before sample introduction is injected to provide a high electric field strength from the beginning of the injection.
Journal ArticleDOI
On-line preconcentration methods for capillary electrophoresis.
TL;DR: A number of different techniques are discussed, including field‐amplified sample stacked, large‐volume sample stacking, pH‐mediated sample stacking), on‐column isotachophoresis, chromatographic preconcentration, sample stacking for micellar electrokinetic chromatography, and sweeping.
Journal ArticleDOI
Sample Stacking in capillary zone electrophoresis : Principles, advantages and limitations
JL Jozef Beckers,Petr Boček +1 more
TL;DR: The principles of stacking procedures are described and their properties are discussed, including the fundamentals of the behavior of zone boundaries and the consequences of the self‐correcting properties of boundaries in moving boundary electrophoresis, isotachophoreis, and zone electrophoreis.
References
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Journal ArticleDOI
Isotachophoresis: the concepts of resolution, load capacity and separation efficiency. I.Theory
TL;DR: In this paper, the authors have discussed the fundamental definitions of resolution and separability in isotachophoresis and extensively discussed the effect of operational parameters, i.e., pH, electrical driving current, sample load and counter constituent on the separation process in terms of resolution time, detection time and load capacity.
Journal ArticleDOI
Conditions for rapid separations of nucleobases and nucleosides by high-pressure anion-exchange chromatography
TL;DR: In this article, the retention behavior of nucleobases (purine and pyrimidine bases) and their nucleosides in systems consisting of water-ethanol mixtures with added electrolytes as mobile phase and a resin-type anion exchanger (Aminex A-28) as stationary phase was systematically investigated.
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Preparative capillary isotachophoresis
TL;DR: Capillary isotachophoresis has been developed and modified for use as a preparative method as discussed by the authors, where the sample zones are pushed out of the capillary under voltage by a counter-flow of leading electrolyte through a small T-tube connection immediately after the UV detector.
Journal ArticleDOI
Capillary isotachophoresis with ultraviolet detection some quantitative aspects
Michal Svoboda,Jiří Vacík +1 more
TL;DR: In this article, the basic theory of UV detector response is presented, and the use of UAV response as a quantitative measure for small amounts of UV absorbing compounds is demonstrated, using this technique complete analysis of mixtures containing components differing in relative amounts by up to four orders of magnitude.
Journal ArticleDOI
Isotachophoresis in narrow-bore tubes. Influence of the diameter of the separation compartment
TL;DR: The importance of the inside diameter of the separation compartment of electrophoretic equipment in which tubes are used in achieving optimal stabilization against conventive disturbances and for limiting the temperature increase is demonstrated.
Related Papers (5)
On-line coupling of capillary isotachophoresis with capillary zone electrophoresis
D. Kaniansky,J. Marák +1 more
Optimization in sample stacking for high-performance capillary electrophoresis
Dean S. Burgi,Ring Ling. Chien +1 more