Dichloran-rose bengal medium for enumeration and isolation of molds from foods.
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Dichloran restricted growth of Rhizopus stolonifer while allowing satisfactory growth of the other test molds, and DRBC restricted the colony size of spreading molds and recovered a wider range of species in higher numbers than other enumeration media.Abstract:
Overgrowth by spreading molds such as Rhizopus and Mucor species is a problem with fungal enumeration media used for foods. Thirty-one antifungal compounds were surveyed for their ability to selectively inhibit such fungi while allowing growth of mycotoxigenic molds and other species of significance in food spoilage. Dichloran (2,6 dichloro-4-nitroaniline) restricted growth of Rhizopus stolonifer while allowing satisfactory growth of the other test molds. Three Rhizopus and Mucor species were encountered that were not inhibited by dichloran; these were controlled by the addition of rose bengal. The optimal medium, designated DRBC, contained 2 micrograms of dichloran and 25 micrograms of rose bengal per ml. DRBC, in pure culture tests and with food samples, restricted the colony size of spreading molds and recovered a wider range of species in higher numbers than other enumeration media.read more
Citations
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Dichloran-glycerol medium for enumeration of xerophilic fungi from low-moisture foods.
Ailsa D. Hocking,John I. Pitt +1 more
TL;DR: A low water activity (alpha omega) medium containing 18% (wt/wt) glycerol and 2 micrograms of dichloran per ml was developed for enumerating the fungal flora of dried and semidried foods and was shown to be significantly better than Christensen malt salt agar when both media were tested with foodstuffs and with pure culture inocula.
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Hypersaline waters in salterns - natural ecological niches for halophilic black yeasts.
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References
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Use of acid, rose bengal and streptomycin in the plate method for estimating soil fungi.
TL;DR: Use of ACID, ROSE BENGAL, and STREPTOMYCin in the plate method for estimating soil productivity and its applications in agriculture and food safety are studied.
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The bacteriostatic action of rose bengal in media used for plate counts of soil fungi
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Stomaching: a new concept in bacteriological sample preparation.
A. N. Sharpe,A. K. Jackson +1 more
TL;DR: An entirely new mixing device, particularly suitable for preparing bacterial suspensions from foods, fabrics, swabs, and other fairly soft materials, has been developed, which is vigorously pounded on its outer surfaces by paddles when placed inside the machine.
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An appraisal of identification methods for Penicillium species: novel taxonomic criteria based on temperature and water relations.
TL;DR: Examination of media and methods currently used in Penicillium taxonomy led to the conclusion that by using only two media, Czapek yeast autolysate agar and malt extract agar, and by incubating cul...