Journal ArticleDOI
Effects of pressure on sedimentation velocity patterns. I. Alpha chymotrypsin at pH 6.2.
Gerson Kegeles,Michael Johnson +1 more
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These computations verify a general prediction, that convective disturbances are to be expected ahead of the moving boundaries when increasing pressure favors the formation of monomer, in this case the slow shoulder previously predicted for the pattern in 0.2 ionic strength phosphate buffer at pH 6.2 largely disappears.About:
This article is published in Archives of Biochemistry and Biophysics.The article was published on 1970-11-01. It has received 13 citations till now. The article focuses on the topics: Hydrostatic pressure.read more
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Book ChapterDOI
Pressure effects in ultracentrifugation of interacting systems.
TL;DR: The evidence is strong that the formation of such bonds in aqueous systems requires a decrease in ordered water structure about the groups involved in bonding leading to a positive volume change of about 10–20 cc/mole bond.
Journal ArticleDOI
Computer simulation of sedimentation in the ultracentrifuge. VI. Monomer-tetramer systems in rapid chemical equilibrium.
TL;DR: In this article, the velocity sedimentation of solutes consisting of a monomer in rapidly established equilibrium with a tetramer has been simulated using a realistic model and a digital computer, and the results are compared with the no-diffusion treatments of the same systems and with the simulated experiments done previously for trimerizing solutes.
Journal ArticleDOI
Molecular sieve studies of interacting protein systems. IX. Reaction boundary profiles for monomer-n-mer systems: comparison with sedimentation.
TL;DR: It was found that bimodal derivative patterns can arise from simultaneous dispersive transport and chemical reaction in the absence of the translational components.
Book ChapterDOI
Sedimentation boundary analysis of interacting systems: use of the apparent sedimentation coefficient distribution function
TL;DR: In this paper, the authors describe methods for the analysis of both self-associating and hetero-ASSociating systems using the apparent distribution function, where s* is the apparent sedimentation coefficient defined as s*=ln(r/rm)/ω 2t and g(s*) has units proportional to concentration per svedberg.
References
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Journal ArticleDOI
A demonstration of some new methods of determining molecular weights from the data of the ultracentrifuge.
Journal ArticleDOI
A boundary anomaly found in the ultracentrifugal sedimentation of mixtures
J. P. Johnston,A. G. Ogston +1 more
Related Papers (5)
Countercurrent distribution of chemically reacting systems. iii. analogs of moving boundary electrophoresis and sedimentation
J. L. Bethune,Gerson Kegeles +1 more