Journal ArticleDOI
Enantiomeric separation of fluorescent, 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate, tagged amino acids.
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TLDR
A new derivatizing reagent, 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate (AQC), has been used successfully for chromatographic enantioseparation of 31 amino acids on cyclodextrin bonded stationary phases.About:
This article is published in Journal of Chromatography A.The article was published on 1993-07-09. It has received 103 citations till now. The article focuses on the topics: Enantiomer & Racemization.read more
Citations
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Journal ArticleDOI
Macrocyclic Antibiotics as a New Class of Chiral Selectors for Liquid Chromatography
TL;DR: In this article, three representative macrocyctic compounds were covalently linked to silica gel and evaluated by HPLC for their ability to resolve racemic mixtures as well as for their stability and loadability.
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Blood-pressure-lowering effect of a novel fermented milk containing gamma-aminobutyric acid (GABA) in mild hypertensives.
TL;DR: FMG may contribute to lowering BP in mildly hypertensive people, and there was a significant decrease of BP within 2 or 4 weeks, and it remained decreased throughout the 12-week intake period.
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A covalently bonded teicoplanin chiral stationary phase for HPLC enantioseparations
TL;DR: It appears that teicoplanin is a widely applicable, highly effective chiral selector for HPLC enantioseparations.
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Facile liquid chromatographic enantioresolution of native amino acids and peptides using a teicoplanin chiral stationary phase
TL;DR: The glycopeptide antibiotic teicoplanin is shown to be a highly effective stationary phase chiral selector for the resolution of underivatized amino-acid and imino-acid enantiomers and the enantioselective separation mechanism is examined using both molecular modeling and retention data.
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Evaluation of the macrocyclic antibiotic vancomycin as a chiral selector for capillary electrophoresis
TL;DR: The physiochemical properties of vancomycin, including its stability in solution, are discussed as they pertain to capillary electrophoresis and Optimization and the effect of different experimental parameters on the enantioselective separations are discussed.
References
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Amino acid analysis utilizing phenylisothiocyanate derivatives.
TL;DR: Using phenylisothiocyanate as the reagent, detection limits under 1 pmol can be routinely achieved, allowing the analysis of submicrogram protein samples, giving the analyst the first realistic alternative to ion-exchange analysis without compromising desirable features of the traditional methodology.
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Appraisal of four pre-column derivatization methods for the high-performance liquid chromatographic determination of free amino acids in biological materials.
TL;DR: Four pre-column derivatization methods, with o-phthaldialdehyde (OPA), 9-fluorenylmethyl chloroformate (FMOC-Cl), phenyl isothiocyanate (PITC) and 1-dimethylaminonaphthalene-5-sulphonyl chloride (dansyl-Cl) were assessed with respect to their applicability in biological research.
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A new approach for the direct resolution of racemic beta adrenergic blocking agents by HPLC
TL;DR: By using an unusual mobile phase consisting of a mixture of polar organic solvents in conjunction with the original native cyclodextrin bonded phase, the following compounds were resolved: propranolol, metoprolol, timolol, ...
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Efficient enantioselective separation and determination of trace impurities in secondary amino acids (i.e., imino acids).
TL;DR: An R-(-)-1-naphthyl)ethyl carbamoylated-beta-cyclodextrin bonded phase in conjunction with a nonaqueous polar mobile phase was used for the highly selective enantioseparation of a number of secondary amino acids after their pre-column derivatization with 9-fluorenylmethyl chloroformate (FMOC).
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High-performance liquid chromatographic enantioseparation of glycyl di- and tripeptides on native cyclodextrin bonded phases: Mechanistic considerations
TL;DR: It is shown that the choice of a suitable cyclodextrin bonded phase used with a nonaqueous polar mobile phase offers a wide range of possibilities to optimize enantioselectivity.