Patent
Enzyme detection method and reagent
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TLDR
In this article, a method for determining nitroreductase activity is proposed, which combines combining a substrate comprising a compound of formula (I): wherein groups Ra and Rb are attached to atoms of the Z ring system, Z represents the a chain of linked atoms necessary to complete an aromatic or hetero-aromatic ring system having five or six atoms selected from carbon atoms and optionally no more than two atoms from oxygen, nitrogen and sulphur.Abstract:
The invention provides a method for determining nitroreductase activity, the method comprising combining a substrate comprising a compound of formula (I): wherein groups Ra and Rb are attached to atoms of the Z ring system; Z represents the a chain of linked atoms necessary to complete an aromatic or heteroaromatic ring system having five or six atoms selected from carbon atoms and optionally no more than two atoms selected from oxygen, nitrogen and sulphur; FLUOR is a fluorescent dye selected from xanthene, coumarin and oxazine dyes; L1 is a bond or is an atom or a chain of 2 to 10 covalently linked atoms selected from the group consisting of carbon, nitrogen, oxygen and sulphur atoms; each Ra is hydrogen or may be selected from electron donating and withdrawing groups, and p is 0 or an integer from 1 to 3; Rb is hydrogen or is the group -L2-W, where L2 is a linker chain containing from 1-20 linked atoms and W is a fluorescent dye moiety or a quenching group; m is 0 or 1; and r is 1 or 2; with a sample containing or suspected to contain nitroreductase under conditions to cause cleavage of FLUOR from the substrate. Nitroreductase activity is determined by detecting a change in an optical property of the sample.read more
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Patent
Method for the Fluorescent Detection of Nitroreductase Activity Using Nitro-Substituted Aromatic Compounds
TL;DR: In this article, the non-fluorescent probes are reduced in the presence of nitroreductase to form fluorescent derivatives that may be detected using fluorescence spectroscopy, which can be used to detect and/or identify a plurality of NERs in a single test environment.
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TL;DR: In this paper, a compound comprising the structure: (SIG)-(SI-MOD)m.m is presented, where SIG is a signaling molecule, SI is a self-immolative structure bound to SIG such that SIG has a reduced signal relative to the signal of SIG without SI, and MOD is a moiety bound to SI that is subject to modification by an activator, and in is an integer from 1 to about 10.
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Nitroreductase-specific fluorescent probe and preparation thereof and application in tumor-targeted fluorescence imaging and monitoring of tumor hypoxia
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Patent
Fluorescence probe for detecting nitroreductase
TL;DR: In this article, an aggregation-induced emission (AIE) type fluorescence probe for detecting nitroreductase has been presented, which is named as [2-(4-nitrophenyl)-1,1,2-triphenyl]ethylene, TPE-NO2 for short.
References
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Patent
Acridone derivatives as labels for fluorescence detection of target materials
TL;DR: In this article, a set of different fluorescent acridone dye derivatives, each dye having a different fluorescence lifetime, were described, and the set of dyes being particularly useful for multiparameter analysis.
Patent
Nitrocoumarins for detecting all micro-organisms
Arthur James,Daniel Monget +1 more
TL;DR: In this article, the use of a compound for detecting the presence or absence of at least a micro-organism has been discussed, where the compound consists of a nitrocoumarin molecule or one of its derivatives.
Patent
Fluorescent detection method and reagent
TL;DR: In this article, a method for increasing the fluorescence of a cyanine dye molecule comprising at least one NO2 group characterised by the reduction of the at least 1 NO 2 group to NHOH or NH2 by the action of a nitroreductase was presented.
Patent
Method and reagent for measuring nitroreductase enzyme activity
TL;DR: In this paper, the authors presented a method for detecting NER activity and gene expression in cellular assays using squaraine reporter dyes and methods for screening for a test agent whose effect upon NER enzyme activity and NER gene expression was determined.
Patent
Quinacridone derivatives as labelling reagents for fluorescence detection of biological materials
TL;DR: The quinacridone derivatives have the following structure: in which Z1 and Z2 independently represent the atoms necessary to complete one ring, two fused ring, or three fused ring aromatic or hetero-aromatic systems, each ring having five or six atoms selected from carbon atoms and optionally no more than two atoms from oxygen, nitrogen and sulphur; R3, R4, R5, R6, R7 and R8 are independently selected from hydrogen, halogen, amide, hydroxyl, cyano, nitro, mono- or di