[Extra-intestinal cycle of Trypanosoma cruzi in species of the genus Panstrongylus, Rhodnius and Triatoma].

Chapter 1 – Trypanosoma rangeli

On two occasions I have reviewed the different aspects of the life cycle of Trypanosoma cruzi in the insect vector. In the first article (Zeledon 1987) I presented a detailed historical appraisal of the subject since the pioneer work done by Carlos Chagas and some years later by Emmanuel Dias in Brazil, through the more recent contributions on the subject. I also discussed some aspects of the host-parasite relationships, including the different factors involved in the cycle. In the second review article (Zeledon 1997), I stressed the microecological factors implicated in the cycle, particularly the role played by cyclic adenosine monophosphate (c AMP), a molecule which seems to be a clue in the metacyclogenesis process of the parasite. I also emphasized the importance of the morphological studies made by us and by others with both transmission and scanning electron microscopy in order to shed light on some of the key aspects of the cycle. In referring to the so called extraintestinal or hemocele cycle of the parasite in the insect we suggested, for the first time in the later review, that some of the forms described by Lacombe (1980), as part of this cycle, could belong to the same microsporidian previously described by Kramer (1972) as Octosporea carloschagasi. The description of this new species by Kramer was based on some of the forms observed by Chagas in the gut of Panstrongylus megistus which were confused by him, and 70 years later by Lacombe, with morphological stages of the life cycle of T. cruzi in the insect. In some of Chagas’ figures, Kramer recognized schizonts, sporonts and spores of the new microsporidian (see Plate 12, figs 83-85 and figs 89-97 of Chagas original paper). I now believe that the intracellular forms and the spheres described in some detail by Lacombe (1980, 1981a,b) in the hemocytes and the cells of the Malphigian tubes, represent at least part of the schizogonic and sporogonic cycles of O. carloschagasi, which were also observed in Triatoma infestans and apparently in other species of triatomines (see for instance figs 21, 22 of Lacombe 1980 and fig. 6 of Lacombe & Santos 1984). In relation to other factors involved in the cycle, some information about the population density of the different morphological stages present in various parts of the intestine of the insect, under certain physiological conditions or time periods, have been published recently (Kollien & Schaub 1997, 1998a, b). Kollien et al. (1998), also confirmed that the flagellates have no tendency to attach themselves by the flagellum to the wall of the midgut as they do in the hindgut. This group of authors also claim that the rectal cuticle of the insect consists of a superficial wax layer (hydrophobic layer) where the flagella of the parasites can attach by electrostatic forces as occurs in vitro (Kleffmann et al. 1998, Schmidt et al. 1998). This would contradict the hypothesis of a more specific type of attachment by hemidesmosomes like structures or by polypeptides as discussed previously (Zeledon 1997).

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[Extra-intestinal cycle of Trypanosoma cruzi in species of the genus Panstrongylus, Rhodnius and Triatoma].

Citations

Chapter 1 – Trypanosoma rangeli

Some morphological and molecular aspects of the life cycle of Trypansoma cruzi in the insect vector