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Journal ArticleDOI

Fine structural studies of apolar mitosis.

Jadwiga Molè-Bajer
- 01 Jan 1969 - 
- Vol. 26, Iss: 4, pp 427-448
TLDR
A fine structural analysis of apolar mitosis induced by chloral hydrate was made on Haemanthus katherinaeBak and the relation of ER to formation of MTs is evident and briefly discussed.
Abstract
A fine structural analysis of apolar mitosis induced by chloral hydrate was made on Haemanthus katherinae Bak. endosperm. Under the influence of chloral hydrate MTs disappear initially and then are formed de novo. Kinetochore fibers grow away from kinetochores and their formation is asynchronous for all chromosomes in the set and also for sister kinetochores. Bundles of MTs forming kinetochore fibers converge toward one of the poorly defined polar regions during formation of kinetochore fibers (metaphase) and in motionless kinetochores. Such MTs increasingly diverge when kinetochores move during anaphase. The relation of ER to formation of MTs is evident and briefly discussed. A continuous transition exists between NE and ER during formation and disintegration of the NE. Some theoretical aspects of these problems were also discussed.

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Kinetochores capture astral microtubules during chromosome attachment to the mitotic spindle: direct visualization in live newt lung cells.

TL;DR: Observations on living mitotic cells directly demonstrate, for the first time, that chromosome attachment results from an interaction between astral microtubules and the kinetochore.
Book ChapterDOI

The evolution of the mitotic spindle.

TL;DR: Signs that possible intermediates in the evolution of the mitotic apparatus still exist among living organisms, rather than having been casualties of the selective process, gives idea that some of the unusual patterns of nuclear division of other lower organisms such as euglenoids, trypanosomes, ciliates, fungi, and sporozoa are of evolutionary significance.
Journal ArticleDOI

Light and electron microscopy of rat kangaroo cells in mitosis. III. Patterns of chromosome behavior during prometaphase.

TL;DR: Chromosome orientation and behavior during prometaphase of mitosis in PtK1 rat kangaroo cells were investigated by cinémicrography and electron microscopy and different patterns of chromosome behavior observed can be explained as a result of synchronous or asynchronous formation of sister-kinetochore fibers.
References
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Journal ArticleDOI

THE USE OF LEAD CITRATE AT HIGH pH AS AN ELECTRON-OPAQUE STAIN IN ELECTRON MICROSCOPY

TL;DR: The stain reported here differs from previous alkaline lead stains in that the chelating agent, citrate, is in sufficient excess to sequester all lead present, and is less likely to contaminate sections.
Journal ArticleDOI

Cell motility by labile association of molecules. The nature of mitotic spindle fibers and their role in chromosome movement.

TL;DR: This article summarizes the current views on the dynamic structure of the mitotic spindle and its relation to mitotic chromosome movements based on measurements of birefringence of spindle fibers in living cells, normally developing or experimentally modified by various physical and chemical agents.
Journal ArticleDOI

Evidence For Four Classes of Microtubules in Individual Cells

TL;DR: It was concluded that micro Tubules are proteinaceous, at least in part, and that microtubules are different in composition from membranes.
Journal ArticleDOI

Organization of microtubules and endoplasmic reticulum during mitosis and cytokinesis in wheat meristems.

TL;DR: The fine-structural changes accompanying mitosis in meristematic cells of the roots and coleoptile tissue of wheat have been studied and microtubules are discussed in relation to the descriptions of mitosis and cytokinesis based on optical microscopy of living cells.
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