Journal ArticleDOI
Hydroxysteroid dehydrogenase activtty in human skin
TLDR
The effects of various factors on the histochemical demonstration of 3β‐hydroxysteroid dehydrogenase activity in human skin were examined by a semiquantitative technique of granule counting.Abstract:
SUMMARY.— The effects of various factors on the histochemical demonstration of 3β-hydroxysteroid dehydrogenase activity in human skin were examined by a semiquantitative technique of granule counting. The optimum concentration of DHA was found to be 0·1 mg./ml. and of NAD 0·3 mg./ml. Vitamin K and nicotinamide enhanced the histochemical demonstration of the enzyme whereas cyproterone and cyproterone acetate inhibited the reaction.read more
Citations
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Journal ArticleDOI
Recent Advances in Androgen Metabolism and Their Relation to the Skin
John S. Strauss,Peter E. Pochi +1 more
TL;DR: The purpose of this review is to discuss some of the new information that has come from these studies and, in particular, those bearing actual or potential relevance to the skin.
Journal ArticleDOI
The metabolism of anbhogens by sebaceous glands
TL;DR: It is shown that sebaeeous glands of normal subjects from areas of tlie body prone to develop acne vulgaris exhibited a characteristic jmttern of NAD-dependent hydroxysteroid dehydrogenase (HSD) activity whieh is summarized in Table I.
Journal ArticleDOI
Survey of the distribution of steroid dehydrogenases in sebaceous glands of human skin.
TL;DR: The results of a survey carried out using human skin to investigate the distribution of 3β‐hydroxysteroid dehydrogenase, 16β‐Hydroxysteroids dehydrogenasing and 17β‐HSD over the skin surface found sebaceous glands were found in greater numbers in the face, scalp and thoracic region.
Journal ArticleDOI
Androgens and acne: a new method for the screening of antiandrogens in human skin
TL;DR: A histochemical method for the screening of antiandrogens using human skin is described and the results with a few compounds reported and compared with the results of bioassay.
Journal ArticleDOI
The in vitro metabolism of 7-alpha-3H dehydroepiandrosterone by human skin.
TL;DR: Slices of skin from human subjects have been shown to transform 3H‐dehydroepiandrosterone predominantly to 4‐androstene‐3,17‐dione and 5‐ androstenes‐3β,17β‐diol, and skin from the shoulder appeared significantly more active than that from the thigh or axilla in metabolizing the dehydro‐epiandresterone.
References
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Journal ArticleDOI
Limitations in the histochemical demonstration of pyridine nucleotide-linked dehydrogenases (nothing dehydrogenase).
H. Zimmermann,A. G. E. Pearse +1 more
TL;DR: The development of histochemical methods for specific pyridine nucleotide-linked dehydrogenases is hindered by the activity described, to which Racker's convenient term "nothing dehydrogenase" has been applied.
Journal ArticleDOI
Histochemical distribution of hydroxysteroid dehydrogenases in human skin.
TL;DR: Twelve pieces of skin were obtained from luiman subjects ; six were from the upper part of the back and six from the forearm or lower limb to demonstrate NAD and NADP dependent hydroxysteroid dehydrogenase activity using techniques described previously.
Journal ArticleDOI
The effect of menadione and phenazine methosulfate on the tetrazolium reduction system under histochemical conditions
TL;DR: Phenazine methosulfate had no suppressive influence and was useful in increasing tetrazolium reduction, but its usefulness is, however, limited unless a soluble factor, possibly DT-(DPNH-TPNH)-diaphorase, can be prevented from diffusing into the medium, and "nothing dehydrogenase" is selectively inactivated.
Related Papers (5)
Histochemical distribution of hydroxysteroid dehydrogenases in human skin.
The distribution of hydroxysteroid dehydrogenase in human sebaceous glands.
Histochemical Demonstration of 17-β-Hydroxysteroid Dehydrogenase by Use of Tetrazolium Salt.∗
Bjarne Pearson,Fred Grose +1 more