Patent
Immobilized magnetic fine particle of physiologically active substance
TLDR
In order to collect the above-mentioned magnetic fine particles or separate the particles from magnetotactic bacteria gathered by culture fluid, the concentration by a micro-centrifuge is carried out and the bacterial walls are dissolved with a lysozyme solution as discussed by the authors.Abstract:
PURPOSE:Immobilized magnetic fine particles of a physiologically active substance, obtained by separating from magnetotactic bacteria, having a large surface area due to the small particle size and very large amount of the supported physiologically active substance. CONSTITUTION:The above-mentioned magnetic fine particles are separated from magnetotactic bacteria and can be used even in a state of either being covered with a coating film consisting mainly of a protein or free from the coating film. In general, the magnetic fine particles having the coating film has an advantage in that the harmony with the living body is good. In order to collect the above-mentioned magnetic fine particles or separate the particles from magnetotactic bacteria gathered by culture fluid, the concentration by a micro-centrifuge is carried out and the bacterial walls are dissolved with a lysozyme solution. The centrifugation may then be repeated. Examples of the kind of the physiologically active substance to be immobilized by the separated magnetic fine particles include enzymes, e.g. hydrolase or oxidoreductase, immunity-related substances, growth factors, nucleic acid-related substances, physiologically active substances derived from plants and other physiologically active substances.read more
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