Journal ArticleDOI
In vitro growth of embryos and callus of coconut palm.
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TLDR
A medium for optimal growth of embryos of Jamaican Tall and Green Malayan Dwarf varieties of coconut palm was developed and aneuploid callus was developed with the chromosome number=8 (normal 2n=32).Abstract:
A medium for optimal growth of embryos of Jamaican Tall and Green Malayan Dwarf varieties of coconut palm was developed. The Liquid basal Murashige and Skoog medium was supplemented with coconut milk, IAA and 2IP. Activated charcoal improved embryo growth on agar medium. A single callus line was initiated from solid endosperm and subcultured on basal Schenk and Hildebrandt medium supplemented with 2 mg per 1 NAA. Attempts at inducing organogenesis in the callus were unsuccessful. No vascular tissue was present. The callus was aneuploid with the chromosome number = 8 (normal 2n = 32).read more
Citations
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In vitro fertilization and embryo rescue
TL;DR: The potential of in vitro fertilization and ovule culture is promising and in some situations may be a viable alternative to parasexual hybridization and somatic embryogenesis.
Journal ArticleDOI
Somatic embryogenesis and plants from zygotic embryos of coconut (Cocos nucifera L.) in vitro.
TL;DR: Explants from stem, leaf and rachilla of mature coconut trees turned green and swelled on Y3 semi-solid basal media supplemented with 2,4-D, K, NAA, BA and activated charcoal.
Journal ArticleDOI
Actin monoubiquitylation is induced in plants in response to pathogens and symbionts.
TL;DR: It is shown for the first time that actins found in root nodules of Phaseolus vulgaris are modified transiently during nodule development by monoubiquitylation, and this modification seems to provide increased stability of the microfilaments to proteolytic degradation and seems to be found in fractions in which the actin cytoskeleton is associated with membranes.
References
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Journal ArticleDOI
A revised medium for rapid growth and bio assays with tobacco tissue cultures
Toshio Murashige,Folke Skoog +1 more
TL;DR: In vivo redox biosensing resolves the spatiotemporal dynamics of compartmental responses to local ROS generation and provide a basis for understanding how compartment-specific redox dynamics may operate in retrograde signaling and stress 67 acclimation in plants.
Journal ArticleDOI
Medium and techniques for induction and growth of monocotyledonous and dicotyledonous plant cell cultures
Roy U. Schenk,A. C. Hildebrandt +1 more
TL;DR: The composition of a medium is described that proved useful to culture callus of a variety of monocotyledonous and dicotylingonous plants and facilitated work with single cells and the enzymatic removal of cell walls in related studies.
Journal ArticleDOI
The Major Groups of Palms and Their Distribution
John Dransfield,Harold E. Moore +1 more
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