Patent
Integral element for analysis of liquids
TLDR
In this paper, a multilayer element for the analysis of liquids such as biochemical and biological liquids is proposed, which includes a reagent layer including a composition that is interactive in the presence of a predetermined substance to be analyzed (analyte) to provide a diffusible, detectable species, and a registration layer that is permeable to the detectable species and within which such species, e.g., a dye, can be detected.Abstract:
The present invention concerns a multilayer element for the analysis of liquids such as biochemical and biological liquids. Elements according to the invention include (1) a reagent layer including a composition that is interactive in the presence of a predetermined substance to be analyzed (analyte) to provide a diffusible, detectable species, and (2) a registration layer that is permeable to the detectable species and within which such species, e.g., a dye, can be detected. Also within the element, preferably between the reagent layer and the registration layer, there can be a radiation-blocking layer, such as an opaque reflecting layer, to enhance detection of the diffusible species within the registration layer. The element can also include a spreading layer, preferably separated from the registration layer by a reagent layer. In operation, a sample of liquid under analysis is applied to the reagent layer of the element or, if present, to a spreading layer. If the sample contains analyte that the element is intended to detect, chemical reaction or other interaction within the reagent layer provides a detectable species that diffuses, via any intervening layers such as a radiation-blocking layer, into the registration layer for detection there, such as by radiometric techniques like reflection spectrophotometry.read more
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Patent
Methods and apparatus for sampling and analyzing body fluid
TL;DR: A sampling device for sampling body fluid includes a lancet for making an incision, a capillary tube for drawing up body fluid from the incision and a test strip affixed to an upper end of the capillary tubes for receiving the fluid as mentioned in this paper.
Patent
Capillary immunoassay and device therefor comprising mobilizable particulate labelled reagents
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Methods and apparatus for expressing body fluid from an incision
TL;DR: In this article, a sample of a body fluid such as blood or interstitial fluid is obtained from a body by lancing a portion of a user's skin, preferably in an area other than a finger tip.
Patent
Immunoassays and devices therefor
Abstract: An analytical test device useful for example in pregnancy testing, comprises a hollow casing (500) constructed of moisture-impervious solid material, such as plastics materials, containing a dry porous carrier (510) which communicates indirectly with the exterior of the casing via a bibulous sample receiving member (506) which protrudes from the casing such that a liquid test sample can be applied to the receiving member and permeate therefrom to the porous carrier, the carrier containing in a first zone a labelled specific binding reagent is freely mobile within the porous carrier when in the moist state, and in a second zone spatially distinct from the first zone unlabelled specific binding reagent for the same analyte which unlabelled reagent is permanently immobilised on the carrier material and is therefore not mobile in the moist state, the two zones being arranged such that liquid sample applied to the porous carrier can permeate via the first zone into the second zone, and the device incorporating means, such as an aperture (508) in the casing, enabling the extent (if any) to which the labelled reagent becomes bound in the second zone to be observed. Preferably the device includes a removable cap for the protruding bibulous member.
Patent
Test device and method for colored particle immunoassay
David E Charlton,Neal W Miller +1 more
TL;DR: In this paper, a test cell and a method for detection of a preselected ligand in a liquid sample such as a body fluid are presented. But the method is limited to a single ligand.
References
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Patent
Automated chemical analyzer
TL;DR: In this paper, a disposable reaction container within which a plurality of prepacked reagents are stored and can be led to one or more reaction (admixing) compartments as required, a portion of each reaction compartment is optically transparent so that at can be utilized as a luvette for optical analysis.
Patent
Method and device for determining the concentration of a material in a liquid
TL;DR: A LAYERED TESTING DEVISE as discussed by the authors is used for quantitatively detecting the presence of a product in a test setup by measuring the effect of the test environment on the end product.
Patent
Rapid methods for assay of enzyme substrates and metabolites
TL;DR: In this article, a method and apparatus for quantitating the amounts of enzyme substrates and metabolites present in biological fluids using spot test techniques is presented. But the method is not suitable for the detection of certain pathological conditions which give rise to abnormal levels of metabolites or enzymes.
Patent
Test for a given constituent in a liquid
TL;DR: In this paper, a reagent-free particle of hydrophilic, absorbent, porous, and particulate material, adhered to a surface of a polyethylene film, is transmitted through a layer of a porous medium through which some of the said component is transmitted to the reagent.