FIG. 5. Photograph of the trypan blue exclusion assay showing the live vs dead cells after 3 min of exposure to plasma treatment sampled at 12 h posttreatment. A ratio of 1:1 of the trypan blue dye (0.4% concentration) to 106 cells in media was used and the mixture was loaded on a hemocytometer. The cells that are dead absorb the trypan blue dye while live cells do not. The difference is clearly observed using a bright field microscope at a magnification of 25 .
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