Lyophilization of cell-free Marek's disease herpesvirus and a herpesvirus from turkeys.
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Cell extracts of the JM and GA strains of Marek's disease herpesv virus and the FC 126 strain of turkey herpesvirus were lyophilized with various stabilizers and virus titers obtained with stabilizer than without stabilizer.Abstract:
Cell extracts of the JM and GA strains of Marek's disease herpesvirus and the FC 126 strain of turkey herpesvirus were lyophilized with various stabilizers. Much higher virus titers were obtained with stabilizer than without stabilizer. Titers increased even further in the case of the Marek's disease virus strains by the addition of a chelating agent, disodium ethylenediaminetetraacetate.read more
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Marek's Disease
TL;DR: Clinical signs observed are paralysis of the legs and wings, with enlargement of peripheral nerves, but nerve involvement is sometimes not seen, especially in adult birds.
Journal ArticleDOI
Control of Marek's disease in the Netherlands. I. Isolation of an avirulent Marek's disease virus (strain CVI 988) and its use in laboratory vaccination trials.
TL;DR: A Marek's disease virus of low pathogenicity was isolated from a flock of chickens and was designated strain CVI 988 and gave excellent protection on subsequent challenge with a virulent virus, even if this challenge was performed shortly after vaccination.
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Characteristics of Marek's disease viruses isolated from vaccinated commercial chicken flocks: association of viral pathotype with lymphoma frequency.
TL;DR: The vvMDVs were obtained from both broiler and layer flocks in five widely separated states but may only recently have become prevalent, since none were represented among 10 MDV isolates obtained before 1975, suggesting that npMDVs may augment turkey-herpesvirus-induced vaccinal immunity.
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Differentiation between strains of Marek's disease virus and turkey herpesvirus by immunofluorescence assays.
V. v. Bülow,P.M. Biggs +1 more
TL;DR: Using the direct immunofluorescence technique Marek's disease virus and turkey herpesvirus infections could be distinguished and there were never any significant differences in the appearance and distribution of antigen in infected cells treated with homologous or heterologous antisera at dilutions of comparable activity.
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Protection of Chickens From Newcastle and Marek's Diseases With a Recombinant Herpesvirus of Turkeys Vaccine Expressing the Newcastle Disease Virus Fusion Protein
Robin W. Morgan,Jack Gelb,Christa Sibilla Schreurs,Dieter Lutticken,John K. Rosenberger,Paul J. A. Sondermeijer +5 more
TL;DR: Recombinant HVT vaccines and the parent HVT strain provided similar levels of protection to chickens challenged with the very virulent RB1B strain of Marek's disease virus, indicating that insertion of foreign sequences into the HVT genome did not compromise the ability of HVT to protect against Marek’s disease.
References
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Isolation from turkeys of a cell-associated herpesvirus antigenically related to Marek's disease virus.
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The influence of certain salts, amino acids, sugars, and proteins on the stability of rickettsiae.
TL;DR: A study of the stability of rickettsiae in solutions of varying composition was undertaken and has led to the development of media that, under certain conditions, appear to be at least as effective as milk in preserving the viability of ricksettsiae.
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Feather follicle epithelium: a source of enveloped and infectious cell-free herpesvirus from Marek's disease.
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Studies on acute Marek's disease. I. Characteristics of isolate GA in chickens.
Eidson Cs,Schmittle Sc +1 more
TL;DR: The present study was done to report on an MD isolate found in Georgia and discuss the pathogenic aspects of this agent, ascertain the optimum age for obtaining plasma from birds experimentally infected with isolate GA, and compare MD in birds injected with plasma or tumor suspension for a series of 9 passages at bi-weekly intervals.
Journal Article
A new member of the herpesvirus group isolated from south american marmosets.
TL;DR: A herpesvirus was isolated from two sick marmoset monkeys, that were believed to be infected with a helminth, found to be different from classical herpes simplex virus and from B virus of old world monkeys, with no detectable cross reactivity.