Methanol Metabolism in Pseudomonad C
B. Stieglitz,R. I. Mateles +1 more
TLDR
Cell suspensions of pseudomonad C, a bacterium capable of growth on meethanol as sole carbon source, were able to oxidize methanol, formaldehyde, and formate, although the rates of oxidation for the latter two compounds were much slower.Abstract:
Cell suspensions of pseudomonad C, a bacterium capable of growth on methanol as sole carbon source, were able to oxidize methanol, formaldehyde, and formate, although the rates of oxidation for the latter two compounds were much slower. The latter compounds also could not serve as sole carbon sources. Through the use of labeled compounds, it was shown that in the presence of methanol, formaldehyde, formate, and bicarbonate were incorporated into trichloroacetic acid-precipitable material. Hexose phosphate synthetase activity was found, indicating the assimilation of methanol via an allulose pathway. No hydroxypyruvate reductase activity was found, nor was any complex membrane structure observed. Such a combination of characteristics has been observed in an obligate methylotroph (Pseudomonas W1), but pseudomonad C can utilize a variety of non-methyl substrates.read more
Citations
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Journal ArticleDOI
Isolation and Characterization of Bacteria That Grow on Methane and Organic Compounds as Sole Sources of Carbon and Energy
TL;DR: The location of methane oxidizers in a dimictic lake indicates that these organisms prefer less than saturating levels of dissolved oxygen, and laboratory experiments confirmed the preference of these organisms for atmospheres containing less oxygen than air.
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The carbon assimilation pathways of Methylococcus capsulatus, Pseudomonas methanica and Methylosinus trichosporium (OB3B) during growth on methane.
TL;DR: A survey was made of the enzymes involved in the regeneration of pentose phosphate during C(1) assimilation via a modified pentose phosphorus cycle, finding two alternative routes for cleavage of fructose 6-phosphate are suggested, one route involves fructose diphosphate aldolase and the other 6- phospho-2-keto-3-deoxygluconate a Aldolase.
Journal ArticleDOI
Bacterial yields on methanol, methylamine, formaldehyde, and formate
TL;DR: Several bacteria utilizing C1‐compounds as sole carbon sources were grown on these substrates in continuous culture and the molar yield values of different bacteria which use the serine pathway decreased as the oxidation levels of the C 1‐growth substrates increased.
Journal ArticleDOI
Continuous Culture Used for Media Optimization
R. I. Mateles,E. Battat +1 more
TL;DR: A technique is described in which continuous culture is used for the optimization of media in terms of growth-supporting ability by observing the reaction of the continuous system to injection of suspected growth-limiting nutrients into the growth vessel.
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