Patent
Method for the production of zymosterol and/or the biosynthetic intermediate and/or subsequent products thereof in transgenic organisms
Christine Lang,Markus Veen +1 more
TLDR
In this article, a method for the production of zymosterol, the biosynthetic intermediate or subsequent products thereof by cultivation of organisms, in particular yeasts, which have an increased lanosterol C14-demethylase activity and an increased HMG-CoA reductase activity, was described.Abstract:
The invention relates to a method for the production of zymosterol the biosynthetic intermediate or subsequent products thereof by cultivation of organisms, in particular yeasts, which have an increased lanosterol C14-demethylase activity and an increased HMG-CoA reductase activity, the nucleic acid constructs necessary for the production of the genetically-modified organisms and the genetically modified organisms, in particular the yeasts themselves.read more
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Patent
Production of sterols in oleaginous yeast and fungi
TL;DR: In this paper, the authors present methods for the production of one or more sterol compounds using engineered oleaginous yeast or fungi that are capable of producing sterol compound.
Patent
Linear dna molecule delivery using pegylated quantum dots for stable transformation in plants
TL;DR: In this paper, a linear nucleic acid molecule of interest is introduced into a cell comprising a cell wall, which is then transformed into a plant cell using polyethylene glycol.
Patent
Production of functionalized linear DNA cassette and quantum dot/nanoparticle mediated delivery in plants
TL;DR: In this paper, the authors introduce a functionalized linear nucleic acid cassette molecule of interest into a plant cell comprising a cell wall using nanoparticles, which can be used for treating or preventing disease in any plant, especially crop plants.
Patent
Genetically modified organisms for the production of lipids
Christine Lang,Andreas Raab +1 more
TL;DR: In this article, an isolated genetically modified non-mammalian organism is presented, where the activity of acyl CoA:sterol acyltransferase/sterol O-acyltransferase (EC 2.3.1.75) is reduced or abolished in comparison with a corresponding wild type organism, methods of use of such an organism, shuttle vehicles for making such an organisms, and methods for producing such an animal.
Patent
Production of non-yeast sterols by yeast
TL;DR: In this paper, the production of 7-dehydrocholesterol, 25-hydroxy ergosterol, and 25hydroxy sterol Δ24-reductases in yeast such as Saccharomyces cerevisiae is discussed.
References
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Journal ArticleDOI
Effects of overproduction of the catalytic domain of 3-hydroxy-3-methylglutaryl coenzyme A reductase on squalene synthesis in Saccharomyces cerevisiae.
TL;DR: The enzyme 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase (H MG-R) is the major rate-limiting enzyme of the mevalonate pathway in many organisms, including yeasts and the enhancement in squalene production coincided with a reduction in growth rate, suggesting that HMG-R has been deregulated.
Journal ArticleDOI
Enhanced sterol-acyl transferase activity promotes sterol accumulation in Saccharomyces cerevisiae.
TL;DR: The results show that sterols can indeed be enriched in yeast by enhancing the capacity of the cells to esterify sterols, and that Are2p constitutes a new member of the karmellae-inducing protein family.
Patent
Method for producing ergosterol and intermediate products thereof by means of recombinant yeasts
TL;DR: In this paper, a method for producing ergosterol and intermediate products thereof by means of recombinant yeasts and plasmids for transforming yeasts was described, which relates to the use of DNA for transforming yeast.
Journal ArticleDOI
Effects of elevated expression of the CYP51 (P45014DM) gene on the sterol contents of Saccharomyces cerevisiae
TL;DR: These results suggested that the levels of cellular sterols are controlled in at least tow stages, and that the concomitant elevation of P450 14DM and ergostadienols desaturase (P450 22DS ) is required to increase the cellular ergosterol content.